A Raf-like MAPKKK gene, GhRaf19, negatively regulates tolerance to drought and salt and positively regulates resistance to cold stress by modulating reactive oxygen species in cotton

Jia, Hongyu; Hao, Lili; Guo, Xing; Liu, Shuchang; Yan, Yan; Guo, Xingqi

doi:10.1016/j.plantsci.2016.07.014

Cited by 54 publications

(45 citation statements)

References 65 publications

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“…higher plants have indicated the role of MAPKs in temperature stress, including low temperature and high temperature (Teige et al, 2004;Sinha et al, 2011;Wu et al, 2014;Jia et al, 2016). Furthermore, as observed with drought-related cisregulatory elements, some PyMAPKKK genes also contained elements related to temperature, such as the LTR motif, which further confirmed that PyMAPKKs respond to low and high temperature at transcript and regulatory levels, exhibiting diverse expression patterns.…”

Section: Expression Analysis Of Pymapkkk Genes When Exposed To Differmentioning

confidence: 53%

“…As indicated in studies in higher plants, drought can seriously limit the productivity and distribution of plants. As the core regulatory components of the MAPK cascade, MAPKKKs participate widely in the response of plants to abiotic stress (Shou et al, 2004;Shitamichi et al, 2013;Virk et al, 2015;Jia et al, 2016). To assess the expression profiles of PyMAPKKK genes under dehydration stress, the samples were treated with different degrees of dehydration and rehydration stress ( Figure 5 and Supplementary Table 2).…”

Section: Expression Analysis Of Pymapkkk Genes When Exposed To Differmentioning

confidence: 99%

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Genome-Wide Identification, Phylogeny, and Expressional Profiles of the Mitogen-Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Pyropia yezoensis

Kong

Cao

et al. 2020

Front. Mar. Sci.

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Mitogen-activated protein kinase kinase kinases (MAPKKKs) are pivotal components of MAPK cascades. Pyropia yezoensis, belonging to Rhodophyta, is an important macroalga growing in the intertidal zone of temperate Japan and China. At present, little is known about MAPKKKs and their potential regulatory mechanisms in response to environmental stressors in red algae, particularly with regard to how this gene family retained its ancient characteristics. In this study, we identified 33 MAPKKK genes in P. yezoensis by bioinformatics analysis. Phylogenetic analysis classified them into Raf, MEKK and ZIK groups, which contained conserved kinase motif with minor variations. The number of intron of all PyMAPKKK is few, up to 3. Regulatory elements prediction indicated that the cis-acting elements included many motifs involved in the stress response. Furthermore, expression analysis of PyMAPKKKs under different stress conditions revealed that numerous PyMAPKKK genes were involved in various signaling pathways with different expression patterns. Additionally, 16 PyMAPKKK genes were validated their expression patterns by Realtime-PCR analysis. An interactome network of PyMAPKKK was constructed to show that most of the interacting proteins were serine/threonine protein kinases, MAPKs, and ubiquitin-binding proteins. Our study provided helpful information for understanding the evolution and function of the MAPKKK family in P. yezoensis.

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Section: Expression Analysis Of Pymapkkk Genes When Exposed To Differmentioning

confidence: 53%

Section: Expression Analysis Of Pymapkkk Genes When Exposed To Differmentioning

confidence: 99%

Genome-Wide Identification, Phylogeny, and Expressional Profiles of the Mitogen-Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Pyropia yezoensis

Kong

Cao

et al. 2020

Front. Mar. Sci.

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“…MAPKKKs have been reported to play a vital role in various stresses tolerance in various species, including Arabidopsis, cotton, rice, and maize 35 – 38 . Amongst these stressors, drought is a major environmental factor limiting productivity and distribution of plants 39 .…”

Section: Resultsmentioning

confidence: 99%

“…To the best of our knowledge, large numbers of MAPKKKs have been implicated in drought stress in previous studies. DSM1 , a Group B Raf-like MAPKKK in rice, and GhRaf19 , a Group C Raf-like MAPKKK in cotton, positively and negatively regulate resistance to drought stress through reactive oxygen species scavenging, respectively 35 , 37 . Libraries made from rice roots under salinity and cold stress have shown maximum transcript abundance of OsMAPKKK64 , a member of the ZIK, which is elevated under drought stress 9 .…”

Section: Discussionmentioning

confidence: 99%

Global Identification, Classification, and Expression Analysis of MAPKKK genes: Functional Characterization of MdRaf5 Reveals Evolution and Drought-Responsive Profile in Apple

Sun

Huang

et al. 2017

Sci Rep

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Mitogen-activated protein kinase kinase kinases (MAPKKKs) are pivotal components of Mitogen-activated protein kinase (MAPK) cascades, which play a significant role in many biological processes. Although genome-wide analysis of MAPKKKs has been conducted in many species, extant results in apple are scarce. In this study, a total of 72 putative MdMAPKKKs in Raf-like group, 11 in ZIK-like group and 37 in MEEK were identified in apple firstly. Predicted MdMAPKKKs were located in 17 chromosomes with diverse densities, and there was a high-level of conservation in and among the evolutionary groups. Encouragingly, transcripts of 12 selected MdMAPKKKs were expressed in at least one of the tested tissues, indicating that MdMAPKKKs might participate in various physiological and developmental processes in apple. Moreover, they were found to respond to drought stress in roots and leaves, which suggested a possible conserved response to drought stress in different species. Overexpression of MdRaf5 resulted in a hyposensitivity to drought stress, which was at least partially due to the regulation of stomatal closure and transpiration rates. To the best of our knowledge, this is the first genome-wide functional analysis of the MdMAPKKK genes in apple, and it provides valuable information for understanding MdMAPKKKs signals and their putative functions.

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“…MAPK pathway is essential for the regulation of transcriptional response under various environmental stress including cold stress . Furthermore, MAPK cascade can be activated by receptors of cold stimulation such as two component signaling pathway .…”

Section: Discussionmentioning

confidence: 99%

De novo transcriptome sequencing of Flammulina velutipes uncover candidate genes associated with cold‐induced fruiting

Guo

et al. 2018

J Basic Microbiol

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To understand molecular mechanism of cold-induced fruiting in Flammulina velutipes, which is one of most popular edible fungi in east Asia, de novo assembly of the F. velutipes transcriptome was carried out. There were 26,888,494 and 26,275,146 clean reads obtained from mycelium and primordia of F. velutipes, respectively. A total of 20,157 unigenes were de novo assembled and 15,058 of them were annotated. Moreover, 7935 unigenes were differentially expressed between mycelium and primordia, 4025 of them were up-regulated and 3910 were down-regulated. GO and KEGG pathway analysis of the differentially expressed unigenes indicated that functional groups associated with two-component signaling pathway, calcium signaling, mitogen-actived protein kinase pathway, molecular chaperones, cell wall and membrane system, play an important role in cold-induced fruiting of F. velutipes. In this work 643 EST-SSRs were identified in 20,157 unigenes and 1560 EST-SSRs primers pairs were designed. Moreover, 5548 and 5955 SNPs were detected in mycelium and primordia, respectively. Consequently, results of this work can serve as a valuable resource for functional genomics study of cold-induced fruiting in F. velutipes.

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A Raf-like MAPKKK gene, GhRaf19, negatively regulates tolerance to drought and salt and positively regulates resistance to cold stress by modulating reactive oxygen species in cotton

Cited by 54 publications

References 65 publications

Genome-Wide Identification, Phylogeny, and Expressional Profiles of the Mitogen-Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Pyropia yezoensis

Genome-Wide Identification, Phylogeny, and Expressional Profiles of the Mitogen-Activated Protein Kinase Kinase Kinase (MAPKKK) Gene Family in Pyropia yezoensis

Global Identification, Classification, and Expression Analysis of MAPKKK genes: Functional Characterization of MdRaf5 Reveals Evolution and Drought-Responsive Profile in Apple

De novo transcriptome sequencing of Flammulina velutipes uncover candidate genes associated with cold‐induced fruiting

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