A Radish Basic Helix-Loop-Helix Transcription Factor, RsTT8 Acts a Positive Regulator for Anthocyanin Biosynthesis

Lim, Sung-Hun; Kim, Da‐Hye; Kim, Jae K.; Lee, Jong-Yeol; Ha, Sun-Hwa

doi:10.3389/fpls.2017.01917

Cited by 69 publications

(58 citation statements)

References 42 publications

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“…Accumulating evidence demonstrates that TT8 is a central component of the well‐conserved complex that controls flavonoid accumulation in various crops (Escaray et al , ; Li et al , ; Li et al , ; Lim et al , ; Nemesio‐Gorriz et al , ; Schaart et al , ). Taking into account the close phylogenetic relationship between Arabidopsis and Brassica , TT8 gene homologs could play comparable roles in Brassica species.…”

Section: Introductionmentioning

confidence: 99%

“…In A. thaliana, a ternary complex (known as the MBW complex) comprising three TF regulators, namely AtMYB123/TT2 (R2R3-MYB), TT8 (basic helix-loop-helix, bHLH) and TTG1 (WD40 protein), plays a key role in activating PA-specific genes in seed coat development (Xu et al, 2014(Xu et al, , 2015. Accumulating evidence demonstrates that TT8 is a central component of the well-conserved complex that controls flavonoid accumulation in various crops (Escaray et al, 2017;Li et al, 2016;Lim et al, 2017;Nemesio-Gorriz et al, 2017;Schaart et al, 2013). Taking into account the close phylogenetic relationship between Arabidopsis and Brassica, TT8 gene homologs could play comparable roles in Brassica species.…”

Section: Introductionmentioning

confidence: 99%

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Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

Zhai

Cai

et al. 2019

Plant Biotechnology Journal

146

131

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Yellow seed is a desirable trait with great potential for improving seed quality in Brassica crops. Unfortunately, no natural or induced yellow seed germplasms have been found in Brassica napus, an important oil crop, which likely reflects its genome complexity and the difficulty of the simultaneous random mutagenesis of multiple gene copies with functional redundancy. Here, we demonstrate the first application of CRISPR/Cas9 for creating yellow‐seeded mutants in rapeseed. The targeted mutations of the BnTT8 gene were stably transmitted to successive generations, and a range of homozygous mutants with loss‐of‐function alleles of the target genes were obtained for phenotyping. The yellow‐seeded phenotype could be recovered only in targeted mutants of both BnTT8 functional copies, indicating that the redundant roles of BnA09.TT8 and BnC09.TT8b are vital for seed colour. The BnTT8 double mutants produced seeds with elevated seed oil and protein content and altered fatty acid (FA) composition without any serious defects in the yield‐related traits, making it a valuable resource for rapeseed breeding programmes. Chemical staining and histological analysis showed that the targeted mutations of BnTT8 completely blocked the proanthocyanidin (PA)‐specific deposition in the seed coat. Further, transcriptomic profiling revealed that the targeted mutations of BnTT8 resulted in the broad suppression of phenylpropanoid/flavonoid biosynthesis genes, which indicated a much more complex molecular mechanism underlying seed colour formation in rapeseed than in Arabidopsis and other Brassica species. In addition, gene expression analysis revealed the possible mechanism through which BnTT8 altered the oil content and fatty acid composition in seeds.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

Zhai

Cai

et al. 2019

Plant Biotechnology Journal

146

131

View full text Add to dashboard Cite

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“…Plant bHLH proteins involved in a wide arrange of biological processes, and participate in the regulation of plant growth and development, abiotic/biotic stress response, hormone signaling, iron homeostasis and secondary metabolism [15][16][17][18][19][20][21]. Nevertheless, researches on bHLH genes regulation in fruit growth and development were limited.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of melon bHLH transcription factors in regulation of fruit development

Bai¹,

Tan²,

Tian³

et al. 2020

Preprint

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Background: The basic helix-loop-helix (bHLH) transcription factor family is one of the largest transcription factor families in plants, and plays crucial roles in plant development. Melon is one of an important horticulture plants, and is an attractive model plant for studying fruit ripening. However, the bHLH gene family of melon has not been identified yet, and functions in fruit growth and ripening are seldom researched. Results: In this study, 118 bHLH genes were identified in the genome of melon. Phylogenetic analysis illustrated that these CmbHLHs could be classified into 16 subfamilies. Intron distribution pattern analysis of bHLH domain found 13 intron distribution patterns in CmbHLHs. CmbHLH genes were unevenly distributed on chromosomes 1 to 12 of the melon genome, and five CmbHLH s were tandem repeat on chromosomes 4 and 8. Expression characters of CmbHLH genes were studied using the transcriptome data. Tissue analysis of indicated CmbHLH32 high expressed in female flowers and early fruit growth stage. Transgenic plant lines of overexpression of CmbHLH32 were constructed, and overexpression of CmbHLH32 result in early fruit ripening compared to the wild type fruit. Conclusions: The bHLH transcription factor family was identified and analyzed for the first time in the melon, overexpression of CmbHLH32 will affect the ripening time of melon fruit, these findings laid a theoretical foundation for further study on the role of bHLH family members in the growth and development of melon .

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“…Most recently, targeted mutation of the TT8 homologs through CRISPR-Cas9 system in Brassica napus also generated yellow-seeded phenotype [12]. Homologs of TT8 gene were also reported to be involved in PAs biosynthesis in diverse plant species, including Medicago truncatula, Lotus corniculatus, and Raphanus sativus [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas9 Mediated Knockout of NtAn1 to Enhance the Lipid Accumulation in Tobacco Seed for Biodiesel Production

Tian¹,

Liu²,

Li³

et al. 2020

Preprint

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Background: Tobacco seed lipid is a promising non-edible feedstock for biodiesel production. In order to meet the increasing demand, achieving high seed lipid content is one of the major goals in tobacco seed production. The TT8 gene and its homologs negatively regulate seed lipid accumulation in Arabidopsis and Brassica species. We speculated that manipulating the homolog genes of TT8 in tobacco could enhance the accumulation of seed lipid.Results: In this present study, we found that the TT8 homolog genes in tobacco, NtAn1a and NtAn1b, were highly expressed in developing seed. Targeted mutagenesis of NtAn1 genes were created by the CRISPR-Cas9 based gene editing technology. Due to the defect of PAs biosynthesis, mutant seeds showed a phenotype of yellow seed coat. Seed lipid accumulation was enhanced by about 18% and 15% in two targeted mutant lines, respectively. Protein content was also significantly increased in mutant seeds. In addition, the seed yield related traits were not affected by the targeted mutagenesis of NtAn1 genes. Thus, the overall lipid productivity of the NtAn1 knockout mutants were dramatically enhanced. Conclusion: Tobacco NtAn1 genes regulate both PAs and lipid accumulation in the process of seed development. Targeted mutagenesis of NtAn1 genes could generate a yellow-seeded tobacco variety with high lipid and protein content. Furthermore, the present results revealed that CRISPR-Cas9 system could be employed in tobacco seed de novo domestication for biodiesel feedstock production.

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A Radish Basic Helix-Loop-Helix Transcription Factor, RsTT8 Acts a Positive Regulator for Anthocyanin Biosynthesis

Cited by 69 publications

References 42 publications

Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

Targeted mutagenesis of BnTT8 homologs controls yellow seed coat development for effective oil production in Brassica napus L.

Genome-wide identification and characterization of melon bHLH transcription factors in regulation of fruit development

CRISPR-Cas9 Mediated Knockout of NtAn1 to Enhance the Lipid Accumulation in Tobacco Seed for Biodiesel Production

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