“…Based on our analysis and research results, we speculated that IS1 subtype might exhibit an immune “hot” phenotype, associated with active immune cells, while IS2 subtype might represent an immune “cold” phenotype, characterized by fewer active immune cells. In the immune “hot” IS1 phenotype, there might be more anti-tumor immune cells, such as CD56 bright natural killer cells, central memory CD8 + T cells, and gamma-delta T cells, which typically exert a positive effect against tumor growth [ [41] , [42] , [43] ]. Conversely, the immune “cold” IS2 phenotype might be more inclined to contain immune cells that promote tumor development, such as Th17 cells [ 44 ].…”