A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein a from Staphylococcus aureus

Dorval, G.; Welsh, Kenneth I.; Wigzell, Hans

doi:10.1016/0022-1759(75)90021-6

Cited by 148 publications

(33 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This assay was performed as described previously (30) in round-bottomed microtiter plates (Cook M24-AR Greiner Ltd.). Cells were transfered into counting tubes and counted in an Intertechnique Gamma Counter (model CG 4000), equipped with a 3-in.…”

Section: Preparation Of Lymphoid Cell Suspensionsmentioning

confidence: 99%

T cell receptors with allo-major histocompatibility complex specificity from rat and mouse. Similarity of size, plasmin susceptibility, and localization of antigen-binding region.

Binz¹,

Wigzell²

1981

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Antisera specific for the constant part Ctau of T cell receptor molecules in mice and rats have been produced in rabbits by immunization with idiotype-positive rat T cell molecules. Such antisera could be shown to react with the majority of normal T lymphocytes from both rats and mice. Mixed leukocyte culture-activated T blasts displayed a higher percentage of positive cells than concanovalin A- or phytohemaglutinin-induced T blasts. Lipopolysaccharide-induced B blasts were not reactive with the antiserum. Lyt-1-,2+,3+ blasts displayed a significantly brighter straining than the corresponding Lyt-1+,2-,3- blasts. Isolation of the reactive molecules from T cells by immunosorption yielded a 70,000-dalton single chain polypeptide as the dominant group of molecules. Plasmin caused the chain to split into 45,000- and 25,000-dalton polypeptides, with the smaller fragment displaying antigen-binding capacity. Molecules identical in size and plasma degradation patterns were isolated from Lyt-1+,2-3- and 1-,2+,3+ blasts. Preliminary functional data supported the view that the antiserum is directed against the constant region Ctau relevant receptor structures on immunocompetent T lymphocytes.

show abstract

Section: Preparation Of Lymphoid Cell Suspensionsmentioning

confidence: 99%

T cell receptors with allo-major histocompatibility complex specificity from rat and mouse. Similarity of size, plasmin susceptibility, and localization of antigen-binding region.

Binz¹,

Wigzell²

1981

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…For quantitation of many samples the test was modified in the following manner (22) . 1 x 101 lymphoid cells in 25 Al were distributed in round bottom microtiter plates (Cooke Laboratory), 25 dal of 1:10 diluted (or as indicated) serum was added into each well and the plates gently shaked and incubated for 1 h at 4°C.…”

Section: Rat Lymphocyte Preparationsmentioning

confidence: 99%

Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens.

Binz¹,

Wigzell²

1975

The Journal of Experimental Medicine

335

131

View full text Add to dashboard Cite

Antigen-binding receptors on T lymphocytes and IgG antibodies with the same antigen-binding specificity as the T-cell receptors display shared or identical idiotypes. This was shown using a system where adult F1 hybrid rats between two inbred strains were inoculated with T lymphocytes from one parental strain. Such F1 hybrid rats produce antibodies directed against idiotypic determinants present on IgG alloantibodies, produced in the T donor genotype strain and with specificity for the alloantigens of the other parental strain. The idiotypic nature of the F1 antialloantibody serum against the parental alloantibodies was demonstrated both by indirect hemagglutination tests or by gel diffusion using alloantisera with different specificity as targets. Furthermore, the F1 anti-T-lymphocyte sera could be shown to contain antibodies against idiotypic parental T lymphocytes as well. This was shown by the capacity of the antisera, in the presence of complement, to wipe out the relevant parental T-cell reactivity against the other parental strain (as measured in MLC or GVH) whilst leaving the T-lymphocyte reactivity against a third, unrelated allogeneic strain intact. These findings demonstrate that F1 hybrid rats inoculated with parental T lymphocytes make anti-idiotypic antibodies directed against both the T cell receptors and IgG alloantibodies of that parental strain with specificity for alloantigens of the other parental strain. In order to prove identity between the anti-idiotypic antibodies against the B and T-cell antigen-binding molecules the following experiments were carried out; highly purified IgG from relevant alloantibody-containing serum in immunosorbent from could be shown to selectively remove both anti-idiotypic activities from the F1 antiserum. Further more, parental normal T lymphocytes could be shown capable of removing from the anti-idiotypic antisera all those antibodies that would cause agglutination of the relevant alloantibody-coated erythrocytes in the indirect agglutination assay. We would thus conclude that T and B lymphocytes reactive against a given antigenic determinant use receptors with antigen-binding areas coded for by the same variable gene subset(s).

show abstract

“…Dorval et al 1975;Langone 1978;Enzmann 1978;Crowther & Abuelzein 1980). However, its uses in many laboratories was; and so predominantly replaced by the handy ELISA technique.…”

Section: Pa -Radioimmunoassay (Pa-ria)mentioning

confidence: 99%

Utilization of the Staphylococcus aureus Protein 'A' and the Streptococcus spp. Protein 'G' in Immunolabelled Techniques

Abuelzein¹

2012

Trends in Immunolabelled and Related Techniques

View full text Add to dashboard Cite

A radioimmunoassay of cellular surface antigens on living cells using iodinated soluble protein a from Staphylococcus aureus

Cited by 148 publications

References 12 publications

T cell receptors with allo-major histocompatibility complex specificity from rat and mouse. Similarity of size, plasmin susceptibility, and localization of antigen-binding region.

T cell receptors with allo-major histocompatibility complex specificity from rat and mouse. Similarity of size, plasmin susceptibility, and localization of antigen-binding region.

Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens.

Utilization of the Staphylococcus aureus Protein 'A' and the Streptococcus spp. Protein 'G' in Immunolabelled Techniques

Contact Info

Product

Resources

About