A Radioimmunoassay Index for Allergic Bronchopulmonary Aspergillosis

Patterson, Roy; Greenberger, Paul A.; Ricketti, Anthony J.; Roberts, Mary

doi:10.7326/0003-4819-99-1-18

Cited by 43 publications

(11 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Circulating antibodies belonging to the IgE immu noglobulin class can be easily detected and quanti tated by radioimmunoassays [18]. However, the need for expensive equipment and the use of radioactive reagents limits the universal availability of this tech nique.…”

Section: Discussionmentioning

confidence: 99%

“…Several methods such as agar gel double diffusion, complement fixa tion, hemagglutination, immunofluorescence, radio immunoassay and enzyme-linked immunosorbent as says (ELISA) have been used in the detection of anti bodies against A.fumigatus[\0, 18,22,24], A number bf antigenic preparations from A. fumigatus strains have been tested with these serological methods. Sig nificant elevation of specific IgG antibodies has been demonstrated in aspergillomas, while higher levels of both IgG and IgE have been reported in allergic bron chopulmonary aspergillosis (ABPA) [15,17,20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Partially Purified Glycoprotein Antigen from <i>Aspergillus fumigatus</i>

Kurup¹,

John²,

Resnick³

et al. 1986

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Culture filtrate antigens of Aspergillus fumigatus were fractionated by isoelectric focusing using a pH gradient of 4–6.5. Three fractions, namely, 18, 19 and 20 were pooled and subjected to immunochemical analysis. It contained a concanavalin A binding glycoprotein. Antibody raised against this component was used to prepare an affinity column of IgG-Sepharose and was used to purify crude culture filtrate antigens. This component produced three precipitin arcs in crossed immunoelectrophoresis using anti-A. fumigatus rabbit serum. This fraction has an isoelectric point of 6.5 and showed three components in two-dimensional electrophoresis with approximate molecular weights of 20, 40 and 80 kilo daltons. This antigen reacted with patient sera in the biotinavidin linked immunosorbent assay and showed high levels of anti-A. fumigatus IgG and IgE antibodies in allergic bronchopulmonary aspergillosis and IgG antibodies in aspergilloma. Both controls and Aspergillus skin test positive asthmatics showed only low levels of specific antibodies. Because of the purity of this antigen, its potential use as a standardized antigen in the detection of antibody is discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Partially Purified Glycoprotein Antigen from <i>Aspergillus fumigatus</i>

Kurup¹,

John²,

Resnick³

et al. 1986

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…Other immunodiffusion assays, such as counterimmunoelectrophoresis (5 ) and radioimmunoprecipitation (13,14 ), are more rapid tests with lower detection limits than the precipitation technique. Although ELISA was shown to be even more sensitive and reliable than immunodiffusion (9,10,15 ), its suboptimal specificity can lead to false-positive results, identifying clinically irrelevant IgG responses.…”

mentioning

confidence: 99%

Quantification of IgG Antibodies to Aspergillus fumigatus and Pigeon Antigens by ImmunoCAP Technology: An Alternative to the Precipitation Technique?

2006

View full text Add to dashboard Cite

“…which contained maximum anti-A. fumigatus IgG activity (8)(9)(10)(11)(12)(13)(14)(15) were pooled and used for testing sera from allergic aspergillosis patients.…”

Section: Time Lmm)mentioning

confidence: 99%

“…Measurement ofanti-A. fumigatus serum IgE and IgG has been used in attempts to distinguish atopic patients with asthma from those with allergicaspergillosis (13,23). Low levels of anti-A.fumigatus IgG can be detected in a high proportion of normal individuals (l), but moderate elevations of specific IgG have been found in most patients with allergic aspergillosis.…”

Section: Introductionmentioning

confidence: 99%

Use of Aspergillus fumigatus Culture Filtrate Fractions in ELISA for the Serological Diagnosis of Allergic Aspergillosis

Khan

Richardson

Crutcher

et al. 1984

Mycoses

View full text Add to dashboard Cite

Summary: Fractions of an Aspergillus fumigatus culture filtrate antigen were compared with the whole antigen in an ELISA for the serodiagnosis of allergic aspergillosis. Fractions were prepared by Sephadex G‐200 chromatography and analyzed by fused‐rocket immunoelectrophoresis against rabbit anti‐A. fumigatus sera and by ELISA against sera from allergic aspergillosis patients. The time‐dependence of antigen and IgG adsorption in the ELISA was determined. Both types of antigen adsorbed rapidly to the solid phase. Adsorption of specific anti‐A. fumigatus IgG to both antigens was rapid and increased with incubation time. Both whole antigen and antigen fractions detected specific IgG in allergic aspergillosis patients. In tests with the whole antigen, all patients with allergic aspergillosis had anti‐A. fumigatus IgG levels higher than those seen in asthmatic patients with precipitins to A. fumigatus. In tests with the antigen fractions, only one serum from the allergic aspergillosis group failed to demonstrate an IgG response higher than that seen in the control group of chronic asthmatics. Zusammenfassung: Fraktionen eines Aspergillus‐fumigatus‐Kulturfiltrat‐Antigens wurden mit dem Gesamtantigen bei der Verwendung des ELISA‐Testes für die Serodiagnose der allergischen Aspergillose verglichen. Die Fraktionen wurden mit der Sephadex G‐200 Chromatographie präpariert. Anschließend wurde eine Analyse durch Immunelektrophorese gegen Anti‐Aspergillus‐fumigatus‐Kaninchenserum und mit dem ELISA‐Test gegen Seren von Patienten mit allergischer Aspergillose durchgeführt. Der zeitliche Ablauf der Antigen‐ und der IgG‐Absorption wurde im ELISA‐Test bestimmt. Beide Antigen‐Typen wurden rasch an die Festphase adsorbiert. Die Adsorption von spezifischem Anti‐Aspergillus‐fumigatus‐IgG erfolgte sehr schnell an beide Antigene und steigerte sich mit der Inkubationszeit. Mit dem Gesamt‐Antigen wie mit Antigen‐Fraktionen ließ sich spezifisches IgG bei dem Patienten mit allergischer Aspergillose nachweisen. Bei Verwendung des Gesamtantigens zeigten alle Patienten mit allergischer Aspergillose höhere Anti‐Aspergillus‐fumigatus‐IgG‐Titer als eine Gruppe von Asthma‐Patienten mit Präzipitinen gegen Aspergillus fumigatus. Bei den Untersuchungen mit den fraktionierten Antigenen war mit der Ausnahme eines Serums ebenfalls ein höherer spezifischer IgG‐Titer in der Gruppe mit allergischer Aspergillose im Vergleich zur Kontrollgruppe chronischer Asthmatiker nachzuweisen.

show abstract

A Radioimmunoassay Index for Allergic Bronchopulmonary Aspergillosis

Cited by 43 publications

References 4 publications

A Partially Purified Glycoprotein Antigen from <i>Aspergillus fumigatus</i>

A Partially Purified Glycoprotein Antigen from <i>Aspergillus fumigatus</i>

Quantification of IgG Antibodies to Aspergillus fumigatus and Pigeon Antigens by ImmunoCAP Technology: An Alternative to the Precipitation Technique?

Use of Aspergillus fumigatus Culture Filtrate Fractions in ELISA for the Serological Diagnosis of Allergic Aspergillosis

Contact Info

Product

Resources

About