A Radioimmunoassay for Human Calcitonin M

Clark, Margaret S.; Boyd, G. W.; Byfield, P. G. H.; Foster, G. V.

doi:10.1016/s0140-6736(69)92389-7

Cited by 108 publications

(24 citation statements)

References 18 publications

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“…Recently it has become possible to determine plasma levels of human calcitonin (hCT) using radioimmunoassay (Clark et al,1969;Tashjian et al, 1970;Dietrich et al, 1970;Deftos, 1971;Heynen et al, 1974), but this radioimmunoassay is not so popular when compared with that of the other polypeptide hormones because of shortness of immunizing antigen. We have reported previously a radioimmunoassay for porcine calcitonin , but in that attempt there was no cross reaction with hCT.…”

mentioning

confidence: 99%

Plasma human calcitonin (hCT) levels in normal and pathologic conditions, and their responses to short calcium or tetragastrin infusion.

et al. 1976

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confidence: 99%

Plasma human calcitonin (hCT) levels in normal and pathologic conditions, and their responses to short calcium or tetragastrin infusion.

et al. 1976

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“…Currently, radioimmunoassay techniques are being developed for the determination of plasma calcitonin levels in humans [5,22]. Confirmation of the physiologic significance of calcitonin in explaining the results of the present report awaits future studies with a sensitive immunoassay procedure.…”

Section: Discussionmentioning

confidence: 53%

Decreased Calcium Tolerance in Nongoitrous Cretins

Anast

Guthrie

1971

Pediatr Res

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ExtractResponse to an exogenous calcium load was studied in 7 thyroid-treated sporadic nongoitrous cretins and in 16 normal children. An intravenous infusion of 10 mg/kg of body weight of calcium as calcium gluconate was infused over a 3-hr period. Serum samples obtained before and at 1.5, 3, 3.5, 4, 5, and 7 hr after the start of the infusion were analyzed for calcium, magnesium, and phosphorus. The base-line calcium, magnesium, and phosphorus values in serum did not differ significantly in the thyroidtreated cretins and in normal children. The infusion of calcium did not alter magnesium levels and no consistent changes were noted in phosphorus levels in either group. Mean increments in calcium levels, however, were significantly greater in the cretins at 1.5, 3, 3.5, and 4 hr after the start of the infusion. The response to calcium infusion in two children with a thyroid-iodine organification defect was similar to that seen in the normal children. Speculation

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“…11 illustrates the mean changes in this study; calcium fell from 9.3 (±0.1) to 7.2 (±0.2) mg/dl; calcitonin fell from 12.5 (±-3.7) to 10.4 (±-2.7) pg/ml. noma, the secretion of this hormone has not been extensively studied in normal subjects (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Accordingly, to pursue such studies, we have developed an immunoprecipitation and immunoextraction procedure which was used to concentrate the calcitonin contained in peripheral blood samples (Fig.…”

Section: Methodsmentioning

confidence: 99%