A radiochemical enzymatic endpoint assay for GTP and GDP

Cerpovicz, Paul F.; Ochs, Raymond S.

doi:10.1016/0003-2697(91)90206-9

Cited by 7 publications

(1 citation statement)

References 19 publications

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“…Hepatocytes were isolated from fed, 150-250 g, male Sprague-Dawley rats and incubated as previously described [24]. Isolated hepatocytes were preincubated at a density of 1x10 6 cells/ml at 37°C in Krebs-Ringer medium and treated with bGH, phenylephrine or vasopressin for different times as indicated in the figure legends.…”

Section: Measurement Of Ins(145)p 3 Productionmentioning

confidence: 99%

The phospholipase C-InsP3 pathway is involved in calcium mobilization induced by growth hormone in hepatocytes

Marrero

Déniz

2004

Growth Hormone & IGF Research

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Section: Measurement Of Ins(145)p 3 Productionmentioning

confidence: 99%

The phospholipase C-InsP3 pathway is involved in calcium mobilization induced by growth hormone in hepatocytes

Marrero

Déniz

2004

Growth Hormone & IGF Research

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Bioluminescent determination of 0.1 picomole amounts of guanine nucleotides

Ford

Vaden

Booth

et al. 1994

J. Biolumin. Chemilumin.

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A bioluminescence procedure for the determination of the guanylates has been optimized to allow measurement of 0.1 pmol amounts. Modifications of the Karl procedure include the use of purified firefly luciferase and nucleoside diphosphate kinase instead of a crude extract of firefly tails, the use of Tricine buffer instead of the inhibitory arsenate buffer, and optimization of the amounts of reagents and incubation times for each of the partial reactions. In the determination of GMP, background values varied widely with different lots of bovine guanylate kinase. Careful selection of a suitable lot of bovine brain guanylate kinase was essential for determination of lower amounts of guanylates. This establishes that selection of guanylate kinase must be based on experimental determination and not reported adenylate kinase activity. The wide variation in background was not eliminated by the inclusion of adenylate kinase inhibitors.

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Small Noncytotoxic Carbon Nano‐Onions: First Covalent Functionalization with Biomolecules

Luszczyn

Płońska‐Brzezińska

Palkar

et al. 2010

Chemistry A European J

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Small carbon nano-onions (CNOs, 6-8 shells) were prepared in high yield and functionalized with carboxylic groups by chemical oxidation. After functionalization these nanostructures were soluble in aqueous solutions. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 tetrazolium (MTS) tests showed excellent cytocompatibility of all CNOs analyzed at 30 and 300 microg mL(-1), so these carbon nanostructures can be safely used for biological applications. The first covalent functionalization of oxidized CNOs (ox-CNOs) with biomolecules, by using biotin-avidin interactions is reported here. Multilayers were prepared on a gold surface by layer-by-layer assembly and the process was monitored by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM). Covalent binding of molecules to the short amine-terminated organosulfur monolayers was assessed by Fourier transform infrared spectroscopy using total attenuated reflactance mode (FT-IR/HATR).

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A radiochemical enzymatic endpoint assay for GTP and GDP

Cited by 7 publications

References 19 publications

The phospholipase C-InsP3 pathway is involved in calcium mobilization induced by growth hormone in hepatocytes

The phospholipase C-InsP3 pathway is involved in calcium mobilization induced by growth hormone in hepatocytes

Bioluminescent determination of 0.1 picomole amounts of guanine nucleotides

Small Noncytotoxic Carbon Nano‐Onions: First Covalent Functionalization with Biomolecules

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