A Radioautographic Technique for Whole Mounts of Muscle Fibers

Kopriwa, Beatrix Markus; Moss, F. P.

doi:10.1177/19.1.51

Cited by 15 publications

(5 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All nuclei tagged with silver grains were assumed to be satellite ceils, because it is generally accepted that muscle nuclei other than those associated with satellite cells are not capable of proliferative activity (Moss and Leblond, 1971). Reported values for the proportion of satellite cells that show proliferative activity appear to vary considerably, with a median value of approximately 20 to 30% (Allbrook et al, 1971;Kopriwa and Moss, 1971;Kelly, 1978;Snow, 1978). The number of satellite cell nuclei as a proportion of total muscle nuclei also varies widely (Schultz, 1974;Schmalbruch and Hellhammer, 1977;Schultz et al, 1978;Snow, 1981), but there is evidence supporting the findings of this study (figure 2) that more satellite ceils are present in younger animals (Allbrook et al, 1971;Cardasis and Cooper, 1975b;Young et al, 1978;Campion et al, 1981Campion et al, , 1982Campion et al, , 1984.…”

Section: Nuclei Number and Proliferative Activitymentioning

confidence: 99%

Muscle Growth and Satellite Cell Proliferative Activity in Obese (OB/OB) Mice

Purchas

Romsos

Allen

et al. 1985

Journal of Animal Science

View full text Add to dashboard Cite

Muscle growth of male obese (ob/ob) and lean mice at 2, 3, 5 and 8 wk were analyzed on the basis of weights of gastrocnemius, plantaris and soleus muscles from each hind leg. The carcasses (prepared by removing skin, viscera, head, feet and tail) were analyzed for fat content so that the effect of phenotype on the relationship between muscle weight and fat-free carcass weight could be assessed. For each age group the obese mice had less muscle relative to fat-free carcass weight than lean mice, with the difference being significant at 3 wk (P less than .05) and 8 wk (P less than .025). The proliferative activity of muscle satellite cells in 2- and 3-wk-old obese and lean mice was measured on isolated muscle fibers by autoradiography. Muscle fiber diameter and number of nuclei/unit length were unaffected by phenotype, but the proportion of muscle nuclei showing proliferative activity was lower (P less than .01) in obese than in lean mice at 2 wk (1.05 vs 1.93%, respectively) and 3 wk of age (.23 vs .59%, respectively). These results are consistent with the suggestion that muscle growth is limited by satellite-cell proliferative activity, although direct evidence for a cause and effect relationship is not provided.

show abstract

Section: Nuclei Number and Proliferative Activitymentioning

confidence: 99%

Muscle Growth and Satellite Cell Proliferative Activity in Obese (OB/OB) Mice

Purchas

Romsos

Allen

et al. 1985

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

“…The methods of Bischoff and Konigsberg largely produced myofibre fragments [83,84], and isolation of intact muscle fibres had required fixation [95,96]. Muscle fibres/fragments could be obtained using collagenase [97], and Anne Bekoff and William Betz combined collagenase digestion and trituration to obtain complete viable muscle fibres [98], which could be maintained in culture [99]. Interested in acetyl choline sensitivity, Bekoff and Betz did not specifically look for satellite cells although they were still present [100].…”

Section: Introductionmentioning

confidence: 99%

The muscle satellite cell at 50: the formative years

2011

View full text Add to dashboard Cite

In February 1961, Alexander Mauro described a cell 'wedged' between the plasma membrane of the muscle fibre and the surrounding basement membrane. He postulated that it could be a dormant myoblast, poised to repair muscle when needed. In the same month, Bernard Katz also reported a cell in a similar location on muscle spindles, suggesting that it was associated with development and growth of intrafusal muscle fibres. Both Mauro and Katz used the term 'satellite cell' in relation to their discoveries. Today, the muscle satellite cell is widely accepted as the resident stem cell of skeletal muscle, supplying myoblasts for growth, homeostasis and repair.Since 2011 marks both the 50th anniversary of the discovery of the satellite cell, and the launch of Skeletal Muscle, it seems an opportune moment to summarise the seminal events in the history of research into muscle regeneration. We start with the 19th-century pioneers who showed that muscle had a regenerative capacity, through to the descriptions from the mid-20th century of the underlying cellular mechanisms. The journey of the satellite cell from electron microscope curio, to its gradual acceptance as a bona fide myoblast precursor, is then charted: work that provided the foundations for our understanding of the role of the satellite cell. Finally, the rapid progress in the age of molecular biology is briefly discussed, and some ongoing debates on satellite cell function highlighted.

show abstract

“…For autoradiographic visualization of AChRs on dissociated fibers, fibers were incubated in suspension in 5 nM 125I-labeled a-BTX in 0.1% bovine serum albumin (125I-a-BTX, New England Nuclear, [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] mCi/mg; 1 Ci = 37 GBq). After labeling, fibers were washed as described above and then resuspended in NTB2 nuclear track emulsion (diluted 1:1 with 50% glycerol; Kodak;ref.…”

Section: Methodsmentioning

confidence: 99%

Neural cell adhesion molecule (N-CAM) is elevated in adult avian slow muscle fibers with multiple terminals.

Bleisch

Scharff²,

Nottebohm³

1989

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Many adult avian muscles contain two types of muscle fiber: those that receive innervation at single focal terminals and those with multiple terminals. The muscles of the syrinx, the vocal organ of birds, are such mixed muscles. To study this heterogeneity of fiber type and innervation, we combined immunocytochemistry to classify muscle fibers with techniques to visualize neuromuscular junctions. One monoclonal antibody, S58, directed against a slow class of myosin, labels only fibers that have multiple terminals. We also examined the distribution of immunoreactivity for neural cell adhesion molecule (N-CAM), which has been suggested to play a role in innervation of muscle and formation of neuromuscular junctions. S58-positive fibers have elevated N-CAM staining, indicating that multiple innervation of a fiber is correlated with the fiber's expression of high levels of N-CAM immunoreactivity. Most, and perhaps all, fibers that have multiple terminals also contain abundant N-CAM immunoreactivity. This suggests that N-CAM may play a role in the maintenance of multiterminal innervation in adult innervated muscle.While fast-twitch muscle fibers are innervated by a single axon at a single focal terminal, many muscle fibers have a distinct pattern of innervation with multiple small terminals (1). The classic comparison of differences in muscle innervation is between the posterior and anterior latissimus dorsi muscles (PLD and ALD) in the chicken (2, 3). The PLD is a fast-twitch muscle of the wing; the ALD is an adjacent postural muscle composed of slow tonic fibers, incapable of fast contractions but able to sustain contractions for much longer than PLD fibers (3). PLD fibers have predominantly "fast" isoforms of myosin and other protein, while ALD fibers contain "slow" isoforms (see ref. 4 for review; refs. 5 and 6). In addition to differences in physiology and biochemistry, these two muscles differ in their pattern of innervation (7). Muscle fibers in the adult PLD are innervated by single motoneurons at single focal end plates, one on each fiber. ALD fibers are innervated by multiple motoneurons and have multiple small terminals scattered along their lengths. The ALD and PLD are composed almost exclusively of multiterminal and single-terminal fibers, respectively. However, there are muscles, such as the biventer cervis, the "complexus" and the sartorius muscles of the chicken (3,4,8), and the extraocular and intrinsic ear muscles of mammals (reviewed in ref. 9) that include both multi-and single-terminal fibers.The present report focuses on the intrinsic muscles of the syrinx and combines techniques of immunocytochemistry to classify muscle fibers with techniques to visualize neuromuscular junctions. MATERIALS AND METHODSMaterials. All muscles were from male and female zebra finches in good health and full adult plumage.Supernatants containing mouse monoclonal antibodies MF30, ALD66, ALD19, ALD58, MF1, and MF20 were generously provided by D. Fischman and D. M. Bader (Cornell University School of Medicine). ...

show abstract

A Radioautographic Technique for Whole Mounts of Muscle Fibers

Cited by 15 publications

References 5 publications

Muscle Growth and Satellite Cell Proliferative Activity in Obese (OB/OB) Mice

Muscle Growth and Satellite Cell Proliferative Activity in Obese (OB/OB) Mice

The muscle satellite cell at 50: the formative years

Neural cell adhesion molecule (N-CAM) is elevated in adult avian slow muscle fibers with multiple terminals.

Contact Info

Product

Resources

About