A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice

An, Yang; Dai, Xianjun; Zhang, Wenhao

doi:10.1093/jxb/err431

Cited by 643 publications

(460 citation statements)

References 53 publications

(97 reference statements)

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“…However, the OsMYB4 network was independent of the DREB/CBF pathway (Park et al, 2010). In addition, several members belonging to the MYB family, including OsMYB2 (Yang et al, 2012), OsMYB511 , CMYB1 , and OsMYB48-1 (Xiong et al, 2014), also were reported for their roles in cold stress regulation, but their molecular mechanisms have not been clearly revealed.…”

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confidence: 99%

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

et al. 2017

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Cold stress is one of the major limiting factors for rice (Oryza sativa) productivity. Several MYB transcriptional factors have been reported as important regulators in the cold stress response, but the molecular mechanisms are largely unknown. In this study, we characterized a cold-responsive R2R3-type MYB gene, OsMYB30, for its regulatory function in cold tolerance in rice. Functional analysis revealed that overexpression of OsMYB30 in rice resulted in increased cold sensitivity, while the osmyb30 knockout mutant showed increased cold tolerance. Microarray and quantitative real-time polymerase chain reaction analyses revealed that a few b-amylase (BMY) genes were down-regulated by OsMYB30. The BMY activity and maltose content, which were decreased and increased in the OsMYB30 overexpression and osmyb30 knockout mutant, respectively, were correlated with the expression patterns of the BMY genes. OsMYB30 was shown to bind to the promoters of the BMY genes. These results suggested that OsMYB30 exhibited a regulatory effect on the breakdown of starch through the regulation of the BMY genes. In addition, application of maltose had a protective effect for cell membranes under cold stress conditions. Furthermore, we identified an OsMYB30-interacting protein, OsJAZ9, that had a significant effect in suppressing the transcriptional activation of OsMYB30 and in the repression of BMY genes mediated by OsMYB30. These results together suggested that OsMYB30 might be a novel regulator of cold tolerance through the negative regulation of the BMY genes by interacting with OsJAZ9 to fine-tune the starch breakdown and the content of maltose, which might contribute to the cold tolerance as a compatible solute.

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confidence: 99%

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

et al. 2017

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“…For instance, salt-tolerant alfalfa plants roots accumulate two-fold more proline than the salt-sensitive plants [134,135]. Additionally, different strategies used to develop transgenic rice plants that accumulate proline and soluble sugars resulted in plants that were protected against drought and osmotic stress [136][137][138].…”

Section: Improving Abiotic Stresses Tolerance In Crop Cultivarsmentioning

confidence: 99%

Sustainable Agriculture—Enhancing Environmental Benefits, Food Nutritional Quality and Building Crop Resilience to Abiotic and Biotic Stresses

Roberts¹,

Mattoo²

2018

Agriculture

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Feeding nutrition-dense food to future world populations presents agriculture with enormous challenges as estimates indicate that crop production must as much as double. Crop production cannot be increased to meet this challenge simply by increasing land acreage or using past agricultural intensification methods. Food production doubled in the past through substantial use of synthetic fertilizer, pesticides, and irrigation, all at significant environmental cost. Future production of nutrition-dense food will require next-generation crop production systems with decreased reliance on synthetic fertilizer and pesticide. Here, we present three case studies detailing the development of cover crops and plant-beneficial microbes for sustainable, next-generation small grain, tomato, and oilseed rape production systems. Cover crops imparted weed and pathogen control and decreased soil erosion and loss of soil nitrogen, phosphorus and carbon, while plant-beneficial microbes provided disease control and phosphorus fertility. However, yield in these next-generation crop production systems at best approximated that associated with current production systems. We argue here that to substantially increase agricultural productivity, new crop germplasm needs to be developed with enhanced nutritional content and enhanced tolerance to abiotic and biotic stress. This will require using all available technologies, including intensified genetic engineering tools, in the next-generation cropping systems.

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“…MYB-core binding sites (T/ CAACNA/G) and MYC-core recognition sites (CANNTG) were noted in the region. Both have been shown to play important roles in plant responses to salt, low temperature, and drought (Miura et al, 2012;Yang et al, 2012;Feng et al, 2013;Su et al, 2014). Furthermore, several hormones could influence the relevant promoter element .…”

Section: Cloning Of the 5'-flanking Region Of The Gpat Gene From Lilimentioning

confidence: 99%

Characterization of the promoter region of the glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum

Chen¹,

Zhang²,

Qi³

et al. 2017

Turk J Biol

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Yadav et al., 2014), while associations between the promoters of these genes and resistance have not yet been reported. Plant promoters play an important role in the regulation of gene expression in plants ( Kim et al., 2013;Reynolds et al., 2013). Promoters are located on the 5'-flanking region of structural genes, recognized by RNA polymerase and combined with template DNA, ensuring that transcription starts effectively and accurately. The promoter consists of two parts: the core region and upstream regulator region (Zhu and Li, 2002). There are abundant cis-acting elements that participate in gene regulation in the promoter (Razdan et al., 2013;Sarvestani et al., 2013). Transient transformation and histochemical staining can contribute to promoter functional analysis (Koia et al., 2013). Cloning and functional studies related to the promoters of the key genes in improving resistance of plants will aid us in understanding its signal transduction pathways and gene expression patterns.According to gene expression, promoters can be divided into two categories: constitutive promoters and specific Abstract: Cold environmental conditions influence the growth and development of plants, causing crop reduction or even plant death. Under stress conditions, cold-inducible promoters regulate cold-related gene expression as a molecular switch. Recent studies have shown that the chloroplast-expressed GPAT gene plays an important role in determining cold sensitivity. However, the mechanism of the transcriptional regulation of GPAT is ambiguous. The 5'-flanking region of GPAT with length of 1494 bp was successfully obtained by chromosome walking from Lilium pensylvanicum. The cis-elements of GPAT promoters were predicted and analyzed by a plant cisacting regulatory DNA element database. There exist core promoter regions including TATA-box and CAAT-box and transcription regulation regions, which involve some regulatory elements such as I-box, W-box, MYB, MYC, and DREB. Full-length and four 5'-deletion fragments linked with GUS vectors were constructed and transformed into Nicotiana tabacum by Agrobacterium-mediated transformation. Transient transformation and histochemical staining of leaves indicated that the activity of the GPAT promoter was strong and induced by low-temperature stress. The deletion of a -294 bp region suggested that the DRE-motif was functionally an essential element for cold induction, and the deletion of -1494 bp and -1194 bp regions suggested that negative regulation exists in the promoter. Our results show that the GPAT gene promoter is a key regulator under cold stress and we think that this study will have significant impact on lily molecular breeding and improving the resistance of plants.

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A R2R3-type MYB gene, OsMYB2, is involved in salt, cold, and dehydration tolerance in rice

Cited by 643 publications

References 53 publications

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

The OsMYB30 Transcription Factor Suppresses Cold Tolerance by Interacting with a JAZ Protein and Suppressing β-Amylase Expression

Sustainable Agriculture—Enhancing Environmental Benefits, Food Nutritional Quality and Building Crop Resilience to Abiotic and Biotic Stresses

Characterization of the promoter region of the glycerol-3-phosphate-O-acyltransferase gene in Lilium pensylvanicum

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