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Shreve, Jack; García-Serrano, M. Victoria; Cash, Annette Grant; Torre, Cristina de la

doi:10.2307/344503

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Rate Equations For the Biocatalytic Reaction1

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2009

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“…The biocatalytic process in (1), is definitely not a direct reaction. It involves several steps and pathways (Soldin and Balinsky, 1968;Shreve and Levy, 1980;Özer et al, 2001), some reversible, the precise kinetics of which is not fully understood, especially for the electrodeimmobilized enzyme as the biocatalyst. We found the output of this reaction sigmoid as a function of the concentration of the substrate, G6P, as illustrated in Figure 4.…”

Section: Rate Equations For the Biocatalytic Reactionmentioning

confidence: 99%

Enzymatic AND-gate based on electrode-immobilized glucose-6-phosphate dehydrogenase: Towards digital biosensors and biochemical logic systems with low noise

Privman

Pedrosa

Melnikov

et al. 2009

Biosensors and Bioelectronics

118

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Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for "digital" (threshold) sensing/actuation. We outline the response functions desirable for such applications and report the first experimental realization of a sigmoid-shape response in one of the inputs. A kinetic model is developed and utilized to evaluate the extent to which the experimentally realized gate is close to optimal.

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Section: Rate Equations For the Biocatalytic Reactionmentioning

confidence: 99%