A quantum dot-based optical immunosensor for human serum albumin detection

Tu, Meng-Che; Chang, Yun-Tzu; Kang, Yu-Ting; Chang, Hwan‐You; Chang, Po‐Cheng; Yew, Tri‐Rung

doi:10.1016/j.bios.2011.11.035

Cited by 64 publications

(20 citation statements)

References 22 publications

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“…The substrate can be modified using amine-terminal silanes , such as aminopropyltriethoxysilane (APTES) (Ghafari and Hanley, 2012;Patel et al, 2011;Tu et al, 2012), 3-isocyanatopropyl triethoxysilane (Szili et al, 2011), 3-aminopropyltrimethoxysilane (APTMS) (Lee et al, 2006;Zhang et al, 2010), and octadecyltrichlorosilane, or thiol-terminal silanes, such as 3-mercaptopropyltrimethoxysilane (MPTMS) (Mansur et al, 2005) and mercaptomethyldimethylethoxysilane (MDS). A cross-linker with two active groups has also been used to covalently immobilize antibodies by conjugation between a silane layer on the surface and the primary amines in the antibody.…”

Section: Covalent Immobilization Of An Antibody By Its Amine Groupmentioning

confidence: 99%

Technological Development of Antibody Immobilization for Optical Immunoassays: Progress and Prospects

Wang

et al. 2014

Critical Reviews in Analytical Chemistry

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The high sensitivity of optical detection techniques and the highly specific reactions between antibodies and antigens mean that optical immunoassays have attracted much interest in the fields of protein, hormone, drug, and microorganism detection, without the need for complex separation and extraction steps. The immobilization of an antibody on a solid support is a crucial step for optical immunoassays. This review surveys the latest advances in current antibody immobilization techniques in detail, including physical adsorption, covalent attachment, bioaffinity immobilization, and some recently developed methods. Furthermore, specific consideration is given to oriented immobilization, which may improve the homogeneous surface covering the accessibility of the active site and surface coverage, and the analytical performance of immunoassays. Finally, new perspectives for antibody immobilization techniques in optical immunoassays are outlined.

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Section: Covalent Immobilization Of An Antibody By Its Amine Groupmentioning

confidence: 99%

Technological Development of Antibody Immobilization for Optical Immunoassays: Progress and Prospects

Wang

et al. 2014

Critical Reviews in Analytical Chemistry

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“…The hollow glass capillaries underwent surface modification to allow for antigen binding on the inner glass wall [32][33][34][35]. The glass capillaries were cleaned by a piranha solution (1:3 H 2 O 2 -concentrated H 2 SO 4 ) before being coated by (3-aminopropyl) triethoxysilane (APTES).…”

Section: Surface Modification Of the Glass Capillariesmentioning

confidence: 99%

Anti-p53 Autoantibody Detection in Automatic Glass Capillary Immunoassay Platform for Screening of Oral Cavity Squamous Cell Carcinoma

Lin

Chen

et al. 2020

Sensors

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The incidence of oral squamous cell carcinoma (OSCC), which is one of the most common cancers worldwide, has been increasing. Serum anti-p53 autoantibody is one of the most sensitive biomarkers for OSCC. Currently, the most commonly used method on clinical screening platforms is the enzyme-linked immunosorbent assay, owing to its high specificity and repeatability. However, conducting immunoassays on 96-well plates is typically time consuming, thereby limiting its clinical applications for fast diagnosis and immediate prognosis of rapidly progressive diseases. The present study performed immunoassays in glass capillaries of 1-mm internal diameter, which increases the surface to volume ratio of the reaction, to shorten the time needed for immunoassay. The immunoassay was automated while using linear motorized stages and a syringe pump. The results indicated that, when compared with the 96-well plate immunoassay, the glass capillary immunoassay decreased the reaction time from typical 120 min to 45 min, reduced the amount of reagent from typical 50 µL to 15 µL, and required only simple equipment setup. Moreover, the limit of detection for glass capillary anti-p53 autoantibody immunoassay was 0.46 ng mL −1 , which is close to the 0.19 ng mL −1 value of the conventional 96-well plate assay, and the glass capillary method had a broader detection range. The apparatus was used to detect the serum anti-p53 autoantibody concentration in clinical patients and compare its results with the conventional 96-well plate method results, which suggested that both of the methods detect the same trend in the relative concentration of serum anti-p53 autoantibody in healthy individuals or patients with OSCC.

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“…[5][6][7][8] Three differents trategies have been reported for the estimation of serum albumins:1 )dye-binding-based optical assays;2 )antibody-based immunochemical methods;a nd 3) LCMS/MS-based proteomic techniques. [9][10][11][12] Of these strategies, the colorimetric assay is the most user-friendlyo ne, because it is the most cost-effective and the least tedious, requiring the minimum number of sample-preparation steps. [13][14][15][16][17][18] Most clinically approved optical methods involveB romocresol green (BCG) as the color-generating dye.…”

Section: Introductionmentioning

confidence: 99%

Motion‐Induced Changes in Emission as an Effective Strategy for the Ratiometric Probing of Human Serum Albumin and Trypsin in Biological Fluids

Dey

Maji

Bhattacharya

2018

Chemistry An Asian Journal

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Herein, we report the formation of a highly luminescent, pH-sensitive, thermoreversible nanoaggregate in pure aqueous medium through the self-agglomeration of carbazole-based amphiphiles. The self-assembly process restricted the intramolecular motion of the molecules and induced a change in its emission signal from blue to cyan, owing to an aggregation-induced emission (AIE) effect. A similar type of ratiometric response was also observed in the presence of human serum albumin (HSA). However, in this case, the molecular motion of the flexible fluorescent probe was restricted by its embedded microenvironment, owing to a motion-induced change in emission (MICE) effect, not by aggregation. Moreover, the probe showed quite high selectivity for HSA over other serum albumin proteins. Our carbazole-based fluorescent probes are a unique example of the ratiometric sensing of HSA through the sole involvement of reversible noncovalent interactions. Considering the important of HSA in clinical diagnosis, a wide range of biological fluids, such as human urine, saliva, and plasma, were screened to analyze their HSA content. In addition, this system was also employed for the detection of trypsin at subnanomolar concentrations through the digestion of HSA.

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A quantum dot-based optical immunosensor for human serum albumin detection

Cited by 64 publications

References 22 publications

Technological Development of Antibody Immobilization for Optical Immunoassays: Progress and Prospects

Technological Development of Antibody Immobilization for Optical Immunoassays: Progress and Prospects

Anti-p53 Autoantibody Detection in Automatic Glass Capillary Immunoassay Platform for Screening of Oral Cavity Squamous Cell Carcinoma

Motion‐Induced Changes in Emission as an Effective Strategy for the Ratiometric Probing of Human Serum Albumin and Trypsin in Biological Fluids

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