A quantitative UHPLC-MS/MS method for the growth hormone-releasing peptide-6 determination in complex biological matrices and transdermal formulations

Esposito, Cloé L.; Ac, Araceli García; Laszlo, Elise; Duy, Sung Vo; Michaud, Catherine; Sauvé, Sébastien; Ong, Huy; Marleau, Sylvie; Banquy, Xavier

doi:10.1016/j.talanta.2021.122555

Cited by 4 publications

(5 citation statements)

References 52 publications

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“…From the perspective of analytical chemistry, MNs sampling coupled with chromatography‐mass spectrometry may contribute to the resolution of the above question, although limited examples have been identified so far. [ 94,95 ]…”

Section: Microneedles (Mns) For Transdermal Diagnosismentioning

confidence: 99%

“…From the perspective of analytical chemistry, MNs sampling coupled with chromatography-mass spectrometry may contribute to the resolution of the above question, although limited examples have been identified so far. [94,95] MNs, as the delivery tool, are used to inject diagnostic agents such as fluorescent or colorimetric probe into the skin dermal layer. [86][87][88][89] For instance, the colorimetric sensing reagents encapsulated in dissolving MNs are delivered in ISF to construct colorimetric tattoo biosensor.…”

Section: Microneedles (Mns) For Transdermal Diagnosismentioning

confidence: 99%

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Recent Progress in Microneedles‐Mediated Diagnosis, Therapy, and Theranostic Systems

Yang

Gong

et al. 2022

Adv Healthcare Materials

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Theranostic system combined diagnostic and therapeutic modalities is critical for the real-time monitoring of disease-related biomarkers and personalized therapy. Microneedles, as a multifunctional platform, are promising for transdermal diagnostics and drug delivery. They have shown attractive properties including painless skin penetration, easy self-administration, prominent therapeutic effects, and good biosafety. Herein, an overview of the microneedles-based diagnosis, therapies, and theranostic systems is given. Four microneedles-based detection methods are concluded based on the sensing mechanism: i) electrochemistry, ii) fluorometric, iii) colorimetric, and iv) Raman methods. Additionally, robust microneedles are suitable for implantable drug delivery. Microneedles-assisted transdermal drug delivery can be primarily classified as passive, active, and responsive drug release, based on the release mechanisms. Microneedles-assisted oral and implantable drug delivery mechanisms are also presented in this review. Furthermore, the key frontier developments in microneedles-mediated theranostic systems as the major selling points are emphasized in this review. These systems are classified into open-loop and closed-loop theranostic systems based on the indirectness and directness of feedback between the transdermal diagnosis and therapy, respectively. Finally, conclusions and future perspectives for next-generation microneedles-mediated theranostic systems are also discussed. Taken together, microneedle-based systems are promising as the new avenue for diagnosis, therapy, and disease-specific closed-loop theranostic applications.

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Section: Microneedles (Mns) For Transdermal Diagnosismentioning

confidence: 99%

Section: Microneedles (Mns) For Transdermal Diagnosismentioning

confidence: 99%

Recent Progress in Microneedles‐Mediated Diagnosis, Therapy, and Theranostic Systems

Yang

Gong

et al. 2022

Adv Healthcare Materials

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“…The more accurate and reliable results from this system make MS technology more widely applied in cosmetic and topical drug research. Many new delivery systems such as microneedle [48], Colloidal nanodispersions [49], Polymeric micelle mediated follicular [50], Sulfhydryl modified polymers [51], and polyamidoamine dendrimers [52] have been evaluated with MS technologies. In addition, LCMS is a very quick and effective tool to determine AI for skin benefits and a great linkage between traditional raw materials from botanical and food resources and skin efficacy validation [53].…”

Section: Mass Spectrometry Technologymentioning

confidence: 99%

Mini Review: Mass Spectrometry Technology for Molecule Distribution inside Skin

Hao¹

2022

Adv Res Dermatol Cosmetics

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The functional ingredients for skin-related products have been highly recognized accompanied by the consumers’ curiosity and instrumental improvement. The targeted location of a functional active ingredient in the skin organelle could be very critical information for its product development, safety evaluation, and benefit claims. Due to those factors, mass spectrometry technology exhibited rapid growth in those research areas because it can accurately and sensitively determine the ingredient molecular weights and locations. In this min review, a summarized figure was created for mass spectrometry technology applications in skin research in the last two decades or so. Compared to other related technologies, the uniqueness and advantage of chromatographic-mass spectrometry and mass spectrometry imaging were also emphasized. Several typical examples from separation, isotopic labeling, and combination with Franz cell were discussed.

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“…In the case of well-equipped high-end instruments (especially HRMS instruments), there are no issues in finding concentrations below 1 ng/mL. The main issue with such compounds is their stability and the excretion time in urine [14][15][16]. The main sample preparation technique for such compounds, like GHRP-2 and GHRP-6, is solid-phase extraction on SCX or WCX sorbents of different volumes.…”

Section: Introductionmentioning

confidence: 99%

“…The main sample preparation technique for such compounds, like GHRP-2 and GHRP-6, is solid-phase extraction on SCX or WCX sorbents of different volumes. Such sample preparation can be used for significant pre-concentration of the sample and its cleanup [14].…”

Section: Introductionmentioning

confidence: 99%

Coordination Ion Spray for Analysis of the Growth Hormones Releasing Peptides in Urine—An Application Study

Temerdashev,

Gashimova,

Azaryan

et al. 2024

Separations

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In this article, a comparison of ionization techniques is provided and discussed. Conventional liquid chromatography with an electrospray ionization source shows higher robustness and repeatability in comparison with liquid chromatography coupled with a coordination ion spray (CIS-MS) source using silver nitrate as the dopant. However, the higher sensitivity and possibility to collect more data in untargeted applications mean CIS-MS is emerging as an instrument used in specific applications. During this research, the limit of detection (LOD) for GHRP-2 and GHRP-6 was established at 0.2 ng/mL, and the lower limit of quantification (LLOQ) was 0.5 ng/mL for CIS-MS. For conventional ESI-MS combined with solid-phase extraction on weak cation exchange columns, the limit of detection was found to be 1 ng/mL, and the lower limit of quantification was 2 ng/mL.

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A quantitative UHPLC-MS/MS method for the growth hormone-releasing peptide-6 determination in complex biological matrices and transdermal formulations

Cited by 4 publications

References 52 publications

Recent Progress in Microneedles‐Mediated Diagnosis, Therapy, and Theranostic Systems

Recent Progress in Microneedles‐Mediated Diagnosis, Therapy, and Theranostic Systems

Mini Review: Mass Spectrometry Technology for Molecule Distribution inside Skin

Coordination Ion Spray for Analysis of the Growth Hormones Releasing Peptides in Urine—An Application Study

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