A quantitative study of the bone marrow in the guinea pig throughout life

Fand, Irwin; Gordon, Albert S.

doi:10.1002/jmor.1051000305

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…It accords with the observation of Fand and G ordon (2), that a steady decrease occurred in the number of nucleated cells per unit weight of femoral marrow, as progression was made chronologically from the neonatal to the immature, mature and senile guinea-pig. It also confirms the importance which is attached to the use of animals of constant weight in quantitative studies of haemopoiesis (15).…”

Section: Resultssupporting

confidence: 70%

Marrow Cellularity in Different Regions of the Guinea-Pig’s Skeleton

Hudson¹

1959

Acta Haematol

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Section: Resultssupporting

confidence: 70%

Marrow Cellularity in Different Regions of the Guinea-Pig’s Skeleton

Hudson¹

1959

Acta Haematol

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“…Lymph nodes of young control animals tended to contain a higher number of cells per mg. The lack of significant difference in spleen and lymph nodes in the older animals is probably due to an age-dependent decrease,in number of lymphocytes (Burke & Harris, 1959;Fand & Gordon, 1957;Ropke, 1977) and fits in well with our findings in bone marrow, peripheral blood and thoracic duct lymph.…”

Section: Discussionsupporting

confidence: 90%

Effects of Homozygosity of the nude (rnu) gene in an inbred strain of rats: Studies of lymphoid and non-lymphoid organs in different age groups of nude rats of LEW background at a stage in the gene transfer

Hougen

Klausen²

1984

Lab Anim

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SummarySeveral age groups of nude homozygous rnu/rnu and heterozygous rnu/+ rats of the same genetic background at an early stage of back-crossing (LEWfMol) were compared as to body and organ weights, histological appearance and cell density of lymphoid organs, haematological values and differential counts of bone marrow and peripheral blood. No thymic tissue was found in the nude animals. 7-week-old nudes were smaller than control animals and had relatively larger nonlymphoid organs and cell-depleted peripheral lymphoid organs. Other age groups showed little difference. Peripheral blood of nude rats showed no signs of lymphopaenia in contrast with the findings in nude mice. The number of thoracic duct lymphocytes was, however, significantly smaller in all age groups of the nude rats, and the bone marrow tended to contain fewer lymphocytes.The athymic nude rat has become more and more popular for immunological and oncological studies (Brooks et al., 1980;Eccles, 1980;Pritchard & Eady, 1980;Salomon, Lynch, Prin, Lascaux & Galinha, 1980; Vos, Kreeftenberg, Kruijt, Kruizinga & Steerenberg, 1980;Colston, Fieldsteel & Dawson, 1981;Marshall & Miller, 1981). 2 mutants, the Rowett and the New Zealand (Festing et al., 1978;Berridge, O'Kech, McNeilage, Heslop & Moore, 1979) nude rat, have been described. The morphology of their lymphoid organs has been studied by several authors (Brooks et al., 1980;Fossum, Smith, Bell & Ford, 1980; Vos, Berkvens & Kruijt, 1980;Douglas-Jones, Nelson, Jansen & Miller, 1981), but generally animals have been of an ill-defined genetic background and studies dealing primarily with the quantitation of lymphoid and non-lymphoid organs have not been published. Therefore, it seemed well warranted to gain information about organ weights, cell density and differential counts of partially inbred nude rats. In the present study quantitations of various organs and cell lines were performed in different age groups of Rowett homozygous rnu/rnu and heterozygous litter mate controls on a partial Lewis background, and homozygous Lewis rats. Materials and methods AnimalsAthymic Rowett rnu/rnu male rats partially backcrossed to a LEW background and their heterozygous rnu/+ litter mates were examined.For reasons of comparison 7-week-old normal +/+ LEW/Mol rats were also included in the study. A total number of 80 animals was used.Breeding pairs were originally obtained from M0llgaard's Breeding Centre Ltd, Ll. Skensved, Denmark. The animals were kept in Makrolon type III cages with stainless steel tops. They were fed autoclaved Altromin 14 I 0 pellets ad libitum. The drinking water, also ad libitum, was distiiled and 2 ppm chlorine added. Sterile cage bedding was u.sed and the animals were kept under SPF conditions. The temperature was 27 ± 1 0 C and the relative humidity 55 ± 5%.The breeding scheme was as described by Snell (I948) for introdUCing a recessive gene into an inbred strain. Nude males, mainly on a Lister background, but mixed up with a number of inbred and outbred rat strains, were mated with LE...

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“…Our studies are concerned with cellularity rather than with the marrow content of bones. The number of cells per unit weight of marrow in the chicken is somewhat lower than marrow cellularity in the guinea pig (Fand and Gordon;1957;Hudson, 19591, rat (Burke and Harris, 1959;Ramsell and Yoffey, 1961), or the mouse marrow (Beran and Tribukait, 1971;Miller and Osmond, 1974). Values, expressed in terms of cells per unit weight rather than unit volume, are essentially comparable because the specific gravity of both the suspending medium or serum and the marrow are close to 1.…”

Section: Discussionmentioning

confidence: 99%

“…The cellular composition of bone marrow in rodents undergoes significant changes during postnatal development and one of the cell populations affected is that of lymphocytes (Fand and Gordon, 1957; Burke and Harris, 1959;Miller and Osmond, 1974). Age-related changes in avian bone marrow have been based on qualitative analysis (Dantschakoff, 1907;Sandreuter, 1951), and other studies have either confined attention to the immediate period around hatching (Burmester et al, 1941) or have compared only the relative proportions of various cell types in the marrow (Lucas and Jamroz, 1961;.…”

mentioning

confidence: 99%

Cellular composition of the bone marrow in the chicken: II. The effect of age and the influence of the bursa of fabricius on the size of cellular compartments

Glick

Rosse

1981

Anat. Rec.

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The blood and bone marrow of New Hampshire chicks were analyzed quantitatively from the time of hatch to 8 weeks of age. Hormonal bursectomy was performed by treating embryonating eggs on the 11th day of incubation with testosterone propionate (TP) which resulted in severe hypogammaglobulinemia and complete atrophy of the bursa of Fabricius. TP-treated birds exhibited some lymphocytopenia, reduced splenic weight, and lack of plasma cells in their bone marrow. The number of cells per milligram bone marrow was comparable in normal and TP-treated birds, falling in the range reported for laboratory rodents. The chick medullary hemopoiesis is characterized by the predominance of erythroblasts throughout early development; granulocytes and lymphocytes represent much smaller cellular compartments than in rodents. In the chick granulocytes tend to decrease after hatch, whereas in rodents they tend to increase. The normal chick shows a temporary increase in marrow lymphocytes after hatch, similar to that observed in some young rodents, but on a much smaller scale. Hormonal bursectomy did not prevent the development of a lymphocyte population in the bone marrow. These cells were fewer in TP-treated birds at hatch and at 4 weeks than in normal birds, but at 8 weeks of age normal and bursectomized chicks had comparable numbers of lymphocytes in their marrow. Although some lymphocytes in avain bone marrow may depend on the bursa of Fabricius for their development, a substantial proportion of bone marrow lymphocytes in the chick are bursa independent. The cell surface phenotype and site of origin of these cells remains to be investigated.

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A quantitative study of the bone marrow in the guinea pig throughout life

Cited by 13 publications

References 37 publications

Marrow Cellularity in Different Regions of the Guinea-Pig’s Skeleton

Marrow Cellularity in Different Regions of the Guinea-Pig’s Skeleton

Effects of Homozygosity of the nude (rnu) gene in an inbred strain of rats: Studies of lymphoid and non-lymphoid organs in different age groups of nude rats of LEW background at a stage in the gene transfer

Cellular composition of the bone marrow in the chicken: II. The effect of age and the influence of the bursa of fabricius on the size of cellular compartments

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