A quantitative real-time reverse transcription PCR (qRT-PCR) assay to detect genome segment 9 of all 26 bluetongue virus serotypes

Maan, Narender S.; Maan, Sushila; Belaganahalli, Manjunatha N.; Pullinger, Gillian D.; Montes, Antonio J. Arenas; Gasparini, Marcela Ribeiro; Guimera, Marc; Nomikou, Kyriaki; Mertens, Peter P. C.

doi:10.1016/j.jviromet.2014.11.012

Cited by 26 publications

(25 citation statements)

References 47 publications

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“…These samples were analysed for the presence of BTV RNA and PPRV RNA using Seg‐9 (Maan, Maan, Belaganahalli, Pullinger et al., )‐ and Seg‐10 (Orru et al., )‐specific and N gene‐specific qRT‐PCR assays, respectively (Batten et al., ). A total of 253 samples (72.70%) were positive for the presence of BTV and 137 samples (39.36%) for PPRV.…”

Section: Details Of Primers Used For Amplification Of N Gene Of Pestementioning

confidence: 99%

Concurrent infection of Bluetongue and Peste-des-petits-ruminants virus in small ruminants in Haryana State of India

Maan

Kumar

Gupta

et al. 2017

Transbound Emerg Dis

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SummaryBluetongue (BT) and peste-des-petits-ruminants (PPR) are major transboundary diseases of small ruminant, which are endemic in India. Testing of bluetongue virus (BTV) and peste-des-petits-ruminants virus (PPRV) from recent outbreaks (2015)(2016) in different regions of Haryana State of India revealed that 27.5% of the samples showed the presence of dual infection of BTV and PPRV. Analysis of Seg-2 of BTV (the serotype-determining protein) showed the presence of BTV-12w in several isolates. However, analysis of N gene fragment amplicons showed that viruses belong to lineage IV were most closely related to a pathogenic strain of PPRV from Delhi. This is the first report of co-circulation of PPRV lineage IV and bluetongue virus serotype 12 in the state. K E Y W O R D SBluetongue, epidemiology, Haryana, peste-des-petits-ruminants virus, India Haryana is one of the 29 states in India, situated in north, and its livestock population consists of 6.08 million buffalos, 1.80 million cattle, 0.36 million sheep and 0.36 million goats, which represents the main livelihood for the majority of the rural population of Haryana.

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Section: Details Of Primers Used For Amplification Of N Gene Of Pestementioning

confidence: 99%

Concurrent infection of Bluetongue and Peste-des-petits-ruminants virus in small ruminants in Haryana State of India

Maan

Kumar

Gupta

et al. 2017

Transbound Emerg Dis

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“…Several real-time PCR assays have been developed for BTV , Orru et al 2006, Shaw et al 2007, Toussaint et al 2007, Hoffmann et al 2009, Schroeder et al 2013, Maan et al 2014). There are fewer real-time PCR assays available for EHDV (Wilson et al 2009, Clavijo et al 2010, Eschbaumer et al 2012).…”

Section: Nucleic Acid Detectionmentioning

confidence: 99%

Diagnostic Tools for Bluetongue and Epizootic Hemorrhagic Disease Viruses Applicable to North American Veterinary Diagnosticians

Wilson

Daniels

Ostlund

et al. 2015

Vector-Borne and Zoonotic Diseases

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This review provides an overview of current and potential new diagnostic tests for bluetongue (BT) and epizootic hemorrhagic disease (EHD) viruses compiled from international participants of the Orbivirus Gap Analysis Workshop, Diagnostic Group. The emphasis of this review is on diagnostic tools available to North American veterinary diagnosticians. Standard diagnostic tests are readily available for BT/EHD viruses, and there are described tests that are published in the World Organization for Animal Health (OIE) Terrestrial Manual. There is however considerable variation in the diagnostic approach to these viruses. Serological assays are well established, and many laboratories are experienced in running these assays. Numerous nucleic acid amplification assays are also available for BT virus (BTV) and EHD virus (EHDV). Although there is considerable experience with BTV reverse-transcriptase PCR (RT-PCR), there are no standards or comparisons of the protocols used by various state and federal veterinary diagnostic laboratories. Methods for genotyping BTV and EHDV isolates are available and are valuable tools for monitoring and analyzing circulating viruses. These methods include RT-PCR panels or arrays, RT-PCR and sequencing of specific genome segments, or the use of next-generation sequencing. In addition to enabling virus characterization, use of advanced molecular detection methods, including DNA microarrays and next-generation sequencing, significantly enhance the ability to detect unique virus strains that may arise through genetic drift, recombination, or viral genome segment reassortment, as well as incursions of new virus strains from other geographical areas.

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“…It can be generally transmitted by biting midges of the genus Culicoides (Bishop et al 2000, Nayduch et al 2014. Twenty six distinct serotypes have been reported (Maan et al 2011(Maan et al , 2014. BTV is an arthropod-borne (Roy and Noad 2006) orbivirus that causes important viral disease mainly in sheep and less frequently in cattle, goats, deer, elk, camels, and wild ruminants (Savini et al 2007, Arenas-Montes et al 2014.…”

Section: Introductionmentioning

confidence: 99%

A serological investigation of Blue Tongue Virus in sheep breeds in Karaman province

Yavru¹,

Avci²,

Yapici³

et al. 2015

EurasianJVetSci

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Karaman ilinde yetiştirilen koyunlarda Blue Tongue Virus enfeksiyonunun serolojik olarak araştırılması Öz Amaç: Bu çalışma Karaman'da bulunan koyun işletmelerinde Blue Tongue Virus'a karşı seroprevalansın belirlenmesi amacı ile yapıldı. Gereç ve Yöntem: Beş farklı işletmeden rastgele seçilen (her birinden 70 adet) toplam 350 koyundan kan serum örnekleri toplandı. Örnekler Blue Tongue Virus'a karşı gelişen antikor varlığı yönünden ticari olarak temin edilen competitive enzyme linked immunosorbent assay (cELISA) ile test edildi. Bulgular: İşletmelerde Blue Tongue Virus'a karşı gelişen antikor prevalansı sırası ile %32.85, %28.57, %25.71, %37.14 ve %41.42 olarak belirlendi. Toplam 350 serum örneğinin 116 (%33.14)'sı Blue Tongue Virus'a spesifik antikor varlığı yönünden cELISA ile pozitif tespit edildi. Öneri: Türkiye'nin iklim şartları Blue Tongue Virus'un vek-tör Culicoides türlerinin yaşamları için uygun olduğundan, koyunlar Blue Tongue Virus yönünden sürekli kontrol edilmelidir.

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A quantitative real-time reverse transcription PCR (qRT-PCR) assay to detect genome segment 9 of all 26 bluetongue virus serotypes

Cited by 26 publications

References 47 publications

Concurrent infection of Bluetongue and Peste-des-petits-ruminants virus in small ruminants in Haryana State of India

Concurrent infection of Bluetongue and Peste-des-petits-ruminants virus in small ruminants in Haryana State of India

Diagnostic Tools for Bluetongue and Epizootic Hemorrhagic Disease Viruses Applicable to North American Veterinary Diagnosticians

A serological investigation of Blue Tongue Virus in sheep breeds in Karaman province

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