“…More recently, the Waterston lab pioneered the use of embryos expressing histone::fluorescent protein fusion proteins and nuclear segmentation and tracking algorithms to semi-automatically track nuclear divisions in confocal fluorescent time lapse recordings , greatly improving the number of embryos that can be analyzed compared to manual methods (Hench et al, 2009) and allowing tracking over much longer time windows than can be achieved by image analysis of DIC images (Hamahashi et al, 2005). Others have extended this approach using alternative image analysis methods Giurumescu et al, 2012;Mace et al, 2013;Santella et al, 2010) and, to-date, semi-automated nuclear tracking algorithms have been used to analyze the invariance of cell cycle timing , early (Pohl and Bao, 2010) and late (Giurumescu et al, 2012) cell movements during embryogenesis, gene expression patterns at single cell resolution Murray et al, 2012;Nair et al, 2013), the variability and robustness of development (Moore et al, 2013;Richards et al, 2013), the effects of gene inhibition at single-cell resolution Boeck et al, 2011;Du et al, 2014;Moore et al, 2013), and regulatory interactions and cell fate choices (Du et al, 2014).…”