A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation

Karetkin, Boris; Guseva, E. V.; Евдокимова, Светлана; Mishchenko, Anastasia S.; Khabibulina, Natalia V.; Grosheva, Veronika D.; Menshutina, Natalia; Panfilov, Victor

doi:10.1007/s11274-019-2665-2

Cited by 11 publications

(11 citation statements)

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“…However, in this model, the measure of interaction is empirical coefficients that are not related to the physiology of microbes. Earlier [ 20 ], an approach characterizing the inhibitory effect of substances formed by one microorganism on another was proposed. This approach allowed us to combine the parameters (inhibition constants or MICs) in the model including the description of probiotic growth.…”

Section: Discussionmentioning

confidence: 99%

“…The competition model developed previously [ 20 ] was applied in the current study with some variations. The basic assumptions of the model are as follows: (a) The concentrations of substances produced by the pathogens do not affect probiotic growth; (b) the competition is related to metabolite formation by the probiotics, which reduces the specific growth rate of the pathogens; (c) the inhibitors formed suppress pathogen growth only; (d) the number of inhibitors is limited; and (e) there is no limitation on the substrate.…”

Section: Methodsmentioning

confidence: 99%

“…In this case, the synergic effect will be aimed at increasing the production of inhibitory substances by probiotics, and the prebiotic substrate should be unconsumable or at least poorly consumable by the pathogen. As a comparative criterion to assess the effectiveness of such compositions, the previously proposed synbiotic factor (SF) can be used, which shows how many times the specific growth rate of the pathogen will decrease as a result of the inhibitory effect of the prebiotics on metabolic products [ 20 ]. The aim of our study is to determine the most effective composition of B. bifidum and various fractions of fructans from burdock and Jerusalem artichoke against Staphylococcus basing on the validated quantitative competition model of probiotics and pathogen.…”

Section: Introductionmentioning

confidence: 99%

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A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

et al. 2021

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A number of mechanisms have been proposed explaining probiotics and prebiotics benefit human health, in particular, probiotics have a suppression effect on pathogen growth that can be enhanced with the introduction of prebiotics. In vitro models enhanced with computational biology can be useful for selecting a composition with prebiotics from new plant sources with the greatest synergism. Water extracts from burdock root and Jerusalem artichoke tubers were purified by ultrafiltration and activated charcoal and concentrated on a rotary evaporator. Fructans were precipitated with various concentrations of ethanol. Bifidobacterium bifidum 8 VKPM AC−2136 and Staphylococcus aureus ATCC 43300 strains were applied to estimate the synbiotic effect. The growth of bifidobacteria and staphylococci in monocultures and cocultures in broths with glucose, commercial prebiotics, as well as isolated fructans were studied. The minimum inhibitory concentrations (MICs) of lactic and acetic acids for the Staphylococcus strain were determined. A quantitative model joining the formation of organic acids by probiotics as antagonism factors and the MICs of pathogens (as the measure of their inhibition) was tested in cocultures and showed a high predictive value (R2 ≥ 0.86). The synbiotic factor obtained from the model was calculated based on the experimental data and obtained constants. Fructans precipitated with 20% ethanol and Bifidobacterium bifidum have the greater synergism against Staphylococcus.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

et al. 2021

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“…Importantly, BB-46 alone did not increase butyrate levels but in combination treatment it did so (109). Furthermore, it has been shown that the combination use of inulin with Bifidobacteria can substantially inhibit the pathogenic bacterium Bacillus cereus (110).…”

Section: Synbioticsmentioning

confidence: 99%

The Role of Butyrate in Attenuating Pathobiont-Induced Hyperinflammation

Chen¹,

Vitetta

2020

Immune Netw

117

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An excessive hyperinflammatory response-caused septic shock is a major medical problem that is associated with pathogenic bacterial infections leading to high mortality rates. The intestinal microbiota and the associated elaborated metabolites such as short chain fatty acid butyrate have been shown to relieve pathogenic bacterial-caused acute inflammation. Butyrate can down-regulate inflammation by inhibiting the growth of pathobionts, increasing mucosal barrier integrity, encouraging obligate anaerobic bacterial dominance and decreasing oxygen availability in the gut. Butyrate can also decrease excessive inflammation through modulation of immune cells such as increasing functionalities of M2 macrophages and regulatory T cells and inhibiting infiltration by neutrophils. Therefore, various approaches can be used to increase butyrate to relieve pathogenic bacterial-caused hyperinflammation. In this review we summarize the roles of butyrate in attenuating pathogenic bacterial-caused hyperinflammatory responses and discuss the associated plausible mechanisms.

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“…The criteria obtained from a quantitative model of co-culture has been suggested for the comparison of symbiotic activity against pathogens in different conditions [39]. Analysis of existing databases on prebiotics and probiotics has shown that there are no such databases containing information about prebiotic activities and the methods for their evaluation.…”

Section: Introductionmentioning

confidence: 99%

Online Database for Retrieval Information about Prebiotics and Their Activity

et al. 2020

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The number of studies aimed at proving the prebiotic properties of certain substances or compositions has been actively increasing, which has led to a large accumulation of scientific information that is fragmented and not systematized. Moreover, a number of criteria have been applied in these studies. The lack of an accessible and convenient information space to compare the obtained results seems to hold back not only scientific development, but also practical development in this field. A database called the «On-line Database of Researches on Activity of Prebiotics» (ODRAP) is presented in this article, which contains information about both prebiotics and some probiotics, that were used in these researches. Currently, ODRAP collects 25 bacteria genera or their combinations, 59 bacteria species, 140 prebiotic substances, 61 prebiotic production companies, 2 methods of fermentation, and 271 analyzed articles from 2001 till 2019. To facilitate access to the database, a special Web-interface was created, which allows any user who opens the Web-page to obtain information about the features and activities of prebiotics, as well as to sort the data by species and genus of bacteria applied in tests, the chemical nature or source of prebiotics, and other parameters. The convenience of the Web-interface is that it allows access to the database, regardless of the user platform and from anywhere, via the Internet.

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A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation

Cited by 11 publications

References 50 publications

A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

A Study on the Synbiotic Composition of Bifidobacterium bifidum and Fructans from Arctium lappa Roots and Helianthus tuberosus Tubers against Staphylococcus aureus

The Role of Butyrate in Attenuating Pathobiont-Induced Hyperinflammation

Online Database for Retrieval Information about Prebiotics and Their Activity

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