A Quantitative Lymph Node Weight Assay for Allogeneic Interactions in the Rat

Abstract

Summary. Current methods of iiifasuring alloRraft rr.sponsp.s ;/i vivo are largely sitbjeftive, qualitative or cuitil)C'rsonif.The incTca.se in weight undergone b>-the popliteal lymph node following the injection of allogeneic cells into the foot-pad has been explored as a possible alternative assay.Lymph node enlargement was found to he an immunologically specific response; in\'arial)ly present where challenge cells bore allo-antigens not shared by the recipient; al)sent in irradiated, tolerant, or syngeneic hosts and specifically altered by prior sensitization. It occurred independently of donor cell proliferation.The kinetics of lymph node enlargement in nonnal recipients were similar in all Ag-B disparate strain combinations tested. Peak lymph node weights were invariably found 4 days after challejige. The degree of enlargement was precisely related to the nnmber of challenge cells and was much greater in Ag-B di.sparate than in Ag-B identical combinations. The magnitude of the response to a given dose of antigen \aried with the strain of the recipient and was highly reproducible.The technique is economical of animals and time and is extremely simple to perform. It fulfik most of the requirements for an ideal quantitati\-e allograft assay.INTRODUCTION.

Suppression of Rat Renal Allograft Rejection by Antigen and Antibody

“…allograft survivors in vivo (Dorsch & Roser 1974). These experiments demonstrated that the response of enhanced animals to graft antigens as presented on leukocytes was comparable in magnitude to that of normal rats (Weiss et al 1978b).…”

“…Often such suppressive activity is associated with T cells. Kilshaw & Brent (1977) and Dorsch & Roser(1977) have presented tnodels of suppression of the rejection of skin allografts in which suppressor T cells appear to operate. Suppressor T cells have been observed in specific and nonspecific suppression of the generation of CTL/nv/Vro (Fitch etal.…”

“…1976, Hodes et al 1976). Kilshaw & Brent (1977) and Dorsch & Roser(1977) have presented tnodels of suppression of the rejection of skin allografts in which suppressor T cells appear to operate. Interestingly, Kilshaw and Brent (1977) observed suppression of skin graft rejection whenTcells were adoptively transferred from mice with intact long standing skin grafts to low dose irradiated syngeneic recipients, although normal cellular immunity was observed in vilro.…”

Immune capability of rats with colorectal carcinoma treated by resection with or without 5‐fluorouracil or levamisole

“…The effects of the neoplasm and its treatment on the ability of the rats to respond to an in vivo allogeneic challenge were investigated by the method of Dorsch and Roser [1974]. One hundred microliters (10' cells) of mitomycin C-treated spleen cells from allogeneic Dark Agouti (DA) and syngeneic Wistar/Wag rats were injected SC into the right and left footpads, respectively, of four Wistar rats in each treatment group of tumor and control animals 4 days prior to sacrifice.…”

Potent S1P receptor agonists replicate the pharmacologic actions of the novel immune modulator FTY720

“…In an in vivo mixed lymphocyte reaction, 15 1 and 19 reduced the alloantigen stimulated increase in the draining popliteal lymph node following footpad injection of Fisher splenocytes into Lewis rats. Mean lymph node weight ratios of intra-subject allogeneic to syngeneic splenocyte challenges for 1 and 19 were 1.0 (0.3 mpk/day sc) and 1.6 (0.5 mpk/day sc), respectively, compared to 3.4 for vehicle controls.…”