A quantitative evaluation of α1, α4, αV and β3 endometrial integrins of fertile and unexplained infertile women during the menstrual cycle. A flow cytometric appraisal

González, Ricardo R.; Palomino, Alberto; Boric, A; Vega, Margarita; Devoto, Luigi

doi:10.1093/humrep/14.10.2485

Cited by 66 publications

(39 citation statements)

References 32 publications

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“…However, endometrium from infertile women can not be used as a control because such endometrium can be considered abnormal in terms of cell adhesion receptors (23,24) and microscopic peritoneal endometriosis (26,27). Therefore, endometrium from infertile women could not be used as a control for comparison with endometrium from endometriotic patients.…”

Section: Discussionmentioning

confidence: 99%

Reduction of apoptosis and proliferation in endometriosis

Béliard

Noël

Foidart

2004

Fertility and Sterility

151

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Objective: To evaluate whether endometriosis could be related to an impaired balance between apoptosis and proliferation, two processes which could be modulated by hormonal status.Design: Immunohistochemical study.Setting: Academic research laboratory. Intervention(s):Endometriotic samples obtained from peritoneum of women aged 26-40 years who were undergoing laparoscopy for pain or infertility. Main Outcome Measure(s):Apoptotic cells were detected with the use of the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. The production of p53 and bcl-2, estrogen and Progesterone (P) receptors, and cellular proliferation were assessed by immunohistochemistry in eutopic and ectopic endometria from 30 patients with endometriosis throughout the menstrual cycle. Results were compared with those from normal endometria from 15 fertile patients. Result(s):Endometriotic lesions were characterized by reduced TUNEL and p53 stainings and by enhanced bcl-2 staining. No correlation between apoptosis and estrogen receptor or P receptor levels was found. A lower amount of steroid receptor was found in endometriotic tissues, without cyclic modulation, compared with the eutopic endometrium. Conclusion(s):Our results suggest that when endometrial tissue is located at ectopic locations, it differs from eutopic endometrium by its proliferation rate, steroid hormone levels, and markers of apoptosis. A reduced sensitivity of endometriotic cells to apoptosis could promote the dissemination and implantation of these cells to ectopic sites.

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Section: Discussionmentioning

confidence: 99%

Reduction of apoptosis and proliferation in endometriosis

Béliard

Noël

Foidart

2004

Fertility and Sterility

151

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“…Integrin a 5 b 1 is specific for FN, although integrins a 3 b 1 , a 4 b 1 , a x b 2 , a v b 3 and a v b 6 can also bind FN. It is also well-known that integrins a 1 b 1 , a 2 b 1 , a 3 b 1 and a v b 1 can bind collagen by different recognition sites, while LN-binding integrins include a 1 b 1 , a 2 b 1 , a 3 b 1 , a 6 b 1 , a 7 b 1 and a 6 b 4 (for review, see Gonzalez et al 1999, Mizejewski 1999, van der Flier & Sonnenberg 2001.…”

Section: Introductionmentioning

confidence: 99%

Expression of extracellular matrix proteins and integrins in rat adrenal gland: importance for ACTH-associated functions

Otis

Campbell

Payet

et al. 2007

Journal of Endocrinology

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The expression of main extracellular matrix (ECM) and their integrins were studied in the adult rat adrenal gland. Collagen I, IV (CI, CIV), laminin (LN) and fibronectin (FN) expression was observed surrounding each glomerulosa cell and as long fibrils between the cords of fasciculata cells. In the medulla, FN was present around chromaffin cells or bordering blood vessels. Integrin a2, a3 and a5 were present mainly in the cortex, while a1 was present in the medulla. In culture, all ECM favoured proliferation of both glomerulosa and fasciculata cells, while protein synthesis was lower on FN and LN in glomerulosa cells. CIV promoted ACTH-induced proliferation whereas FN favoured ACTH-induced protein synthesis in glomerulosa cells. Except for LN, ECM increased expression of 3b-hydroxysteroid dehydrogenase and enhanced basal aldosterone, although corticosterone secretion was only enhanced by CI and CIV. In fasciculata cells, the potency of ACTH-induced cAMP production was lower on ECM, compared with plastic. Moreover, ACTH, but not ECM, activated mitogenic-activated protein kinase p38 and stress-activated protein kinases. Glomerulosa and fasciculata cells grown on CI and CIV had a polygonal morphology, while cells grown on LN appeared as clusters of small rounded cells. On FN, the glomerulosa cells exhibited polygonal morphology while fasciculata cells appeared as clusters of small rounded cells. Together, these results indicate that ECM modulates basal and ACTH-induced cell functions, with FN, CI and CIV specifically favouring steroid secretion, as opposed to LN which inhibits secretion while promoting proliferation.

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“…Human ESCs were isolated and cultured as described by González and coworkers (Gonzalez et al 1999). Briefly, tissue samples were minced into small pieces and digested in a two-step process.…”

Section: Human Esc Culturementioning

confidence: 99%

Adiponectin limits differentiation and trophoblast invasion in human endometrial cells

Duval

Santos

Moindjie

et al. 2017

Journal of Molecular Endocrinology

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Successful human embryo implantation requires a proper differentiation of endometrial stromal cells (ESCs) into decidual cells, during a process called decidualization. ESCs express specific molecules, such as prolactin, insulin-like growth factor-binding protein-1 (IGFBP-1) and connexin-43. Decidual cells are also involved in the control of trophoblast invasion, by secreting various factors, such as matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). Adiponectin is an adipokine with insulin-sensitizing, anti-inflammatory and anti-proliferative effects. At the embryomaternal interface, adiponectin promotes differentiation and invasion of human trophoblastic cells. We hypothesize that the effects of adiponectin on endometrium could counteract its pro-invasive effects previously described in the human trophoblast. In this context, we have firstly demonstrated that adiponectin downregulates IGFBP-1 and connexin-43 mRNA expressions, as well as prolactin secretion in ESCs, suggesting an anti-differentiative effect of adiponectin. Secondly, we found that invasive capacities of trophoblastic cell line HTR-8/SVneo are reduced in the presence of conditioned media from ESC cultured in the presence of adiponectin. Adiponectin's anti-invasive action is associated with a decreased activity of MMP-2 and MMP-9, and an increased TIMP-3 mRNA expression in ESCs. Finally, adiponectin receptors (ADIPOR1 and ADIPOR2) knockdown abolishes the anti-differentiative and anti-invasive effects of adiponectin in human ESCs. Altogether, our results suggest that adiponectin reduces the decidualization process and inversely induces the production of endometrial factors that limit trophoblast invasion. Thus, through a dual control in trophoblast and endometrial cells, adiponectin appears as a pivotal actor of the embryo implantation process.

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A quantitative evaluation of α1, α4, αV and β3 endometrial integrins of fertile and unexplained infertile women during the menstrual cycle. A flow cytometric appraisal

Cited by 66 publications

References 32 publications

Reduction of apoptosis and proliferation in endometriosis

Reduction of apoptosis and proliferation in endometriosis

Expression of extracellular matrix proteins and integrins in rat adrenal gland: importance for ACTH-associated functions

Adiponectin limits differentiation and trophoblast invasion in human endometrial cells

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