A quantitative description of the peptide sharing between poliovirus and<i>Homo sapiens</i>

Novello, Giuseppe; Capone, Giovanni; Fasano, Candida; Bavaro, Simona L.; Polito, Anna Nunzia; Kanduc, Darja

doi:10.3109/08923973.2011.608360

Cited by 6 publications

(7 citation statements)

References 50 publications

(71 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In conclusion, the present study confirmed and extended previous reports from this laboratory and others presenting evidence for a massive peptide overlap between viral and human proteomes. Having as a reference frame such a corpus of data, here we detailed the peptide commonality between WNV and the human proteome.…”

Section: Discussionsupporting

confidence: 92%

Evidence for a vast peptide overlap between West Nile virus and human proteomes

Capone

Pagoni

Delfino

et al. 2012

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

The primary amino acid sequence of West Nile virus (WNV) polyprotein, GenBank accession number M12294, was analyzed by computional biology. WNV is a mosquito-borne neurotropic flavivirus that has emerged globally as a significant cause of viral encephalitis in humans. Using pentapeptides as scanning units and the perfect peptide match program from PIR International Protein Sequence Database, we compared the WNV polyprotein and the human proteome. WNV polyprotein showed significant sequence similarities to a number of human proteins. Several of these proteins are involved in embryogenesis, neurite outgrowth, cortical neuron branching, formation of mature synapses, semaphorin interactions, and voltage dependent L-type calcium channel subunits. The biocomputional study suggest that common amino acid segments might represent a potential platform for further studies on the neurological pathophysiology of WNV infections.

show abstract

Section: Discussionsupporting

confidence: 92%

Evidence for a vast peptide overlap between West Nile virus and human proteomes

Capone

Pagoni

Delfino

et al. 2012

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this scenario, new vaccine formulations and renewed research efforts might help to specifically fight PV infection. Recently, we analyzed the peptide overlap between PV1, strain Mahoney, and the human proteome and described a high extent of peptide sharing involving human proteins linked to fundamental cellular functions [ 9 ]. These data appeared to be of interest also because PV has been studied mainly at the nucleotide level [ 10 ], and, in general, there is a lack of knowledge of the interaction(s) between PV and the human host at the peptide/protein level.…”

Section: Introductionmentioning

confidence: 99%

“…Following the mathematical quantification of pentapeptide sharing between PV1 and human proteins, we reported that 2,040 out of the 2,204 pentamers composing the PV1 proteome are shared with the human proteome for a total of 18,223 matches, including multiple occurrences [ 9 ]. In general, a vast peptide sharing with human proteins is a characteristic of viral proteomes [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Applying the Concept of Peptide Uniqueness to Anti-Polio Vaccination

Kanduc

Fasano

Capone

et al. 2015

Journal of Immunology Research

Self Cite

View full text Add to dashboard Cite

Background. Although rare, adverse events may associate with anti-poliovirus vaccination thus possibly hampering global polio eradication worldwide. Objective. To design peptide-based anti-polio vaccines exempt from potential cross-reactivity risks and possibly able to reduce rare potential adverse events such as the postvaccine paralytic poliomyelitis due to the tendency of the poliovirus genome to mutate. Methods. Proteins from poliovirus type 1, strain Mahoney, were analyzed for amino acid sequence identity to the human proteome at the pentapeptide level, searching for sequences that (1) have zero percent of identity to human proteins, (2) are potentially endowed with an immunologic potential, and (3) are highly conserved among poliovirus strains. Results. Sequence analyses produced a set of consensus epitopic peptides potentially able to generate specific anti-polio immune responses exempt from cross-reactivity with the human host. Conclusion. Peptide sequences unique to poliovirus proteins and conserved among polio strains might help formulate a specific and universal anti-polio vaccine able to react with multiple viral strains and exempt from the burden of possible cross-reactions with human proteins. As an additional advantage, using a peptide-based vaccine instead of current anti-polio DNA vaccines would eliminate the rare post-polio poliomyelitis cases and other disabling symptoms that may appear following vaccination.

show abstract

“…Microbial proteomes massively overlap human proteins [34][35][36][68][69][70] in a tight network of metabolic interactions that may carry pathologic sequelae in the host. Indeed, bacterial organisms may interfere with the apoptotic apparatus [71], manipulate and subvert host-cell pathways [72], and interfere with the host immune system and mechanisms [73].…”

Section: Conclusion and Future Perspectivementioning

confidence: 99%