A Quantitative Cytochemical Study of Glutamate and Glucose-6-Phosphate Dehydrogenase Activities during Chilling Injury in Tubers of<i>Dioscorea rotundata</i>Poir.

Onyia, G. O. C.; Gahan, P. B.

doi:10.1093/jxb/36.8.1249

Cited by 3 publications

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Quantitative enzyme cytochemistry in plant biotechnology

Gahan

1991

Phytochemical Analysis

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Immunocytochemistry, one of the two basic approaches to the in situ study of enzymes, allows the enzyme molecules to be localized, but indicates nothing about their activity. Quantitative cytochemistry, on the other hand, indicates the sites of localization of enzyme activity and, in many cases, the rates of these activities. This latter approach, which is the subject of this review, permits the assessment of activity in individual cells or groups of cells in a given tissue. Details of tissue preparation, enzyme procedures, methods of quantification and some case histories illustrate the specificity and flexibility of the method.

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Quantitative enzyme cytochemistry in plant biotechnology

Gahan

1991

Phytochemical Analysis

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Viability of Plant Protoplasts

Gahan

1989

Plant Protoplasts and Genetic Engineering I

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In situ staining of activities of enzymes involved in carbohydrate metabolism in plant tissues

Сергеева

Vreugdenhil²

2002

Journal of Experimental Botany

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A powerful technique is described to localize the activities of a range of enzymes in a wide variety of plant tissues. The method is based on the coupling of the enzymatic reaction to the reduction of NAD and subsequent reduction and precipitation of nitroblue tetrazolium. Enzymes that did not reduce NAD could be visualized by coupling their activities to glucose-6-phosphate dehydrogenase activity via one or more intermediary 'coupling' enzymes. The method is shown to be applicable for the detection of the activities of hexokinase, fructokinase, sucrose synthase, uridine 5'-diphospho-glucose pyrophosphorylase, ADP-glucose pyrophosphorylase, phosphoglucomutase, and phosphoglucose isomerase. It could be used for all tissues tested, including green leaves, stems, roots, fruits, and seeds. The method is specific, very sensitive, and has a high spatial resolution, giving information at the cellular and the subcellular level. The localization of sucrose synthase, invertase, and uridine 5'-diphospho-glucose pyrophosphorylase in transgenic potato plants, carrying a cytokinin biosynthesis gene, is studied and compared with wild-type plants.

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A Quantitative Cytochemical Study of Glutamate and Glucose-6-Phosphate Dehydrogenase Activities during Chilling Injury in Tubers ofDioscorea rotundataPoir.

Cited by 3 publications

References 0 publications

Quantitative enzyme cytochemistry in plant biotechnology

Quantitative enzyme cytochemistry in plant biotechnology

Viability of Plant Protoplasts

In situ staining of activities of enzymes involved in carbohydrate metabolism in plant tissues

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