A quantitative comparable study on multi-hierarchy conformation of acid and pepsin-solubilized collagens from the skin of grass carp (Ctenopharyngodon idella)

Zhu, Shichen; Yuan, Qijuan; Yang, Mingtao; You, Juan; Yin, Tao; Gu, Zhipeng; Hu, Yang; Xiong, Shanbai

doi:10.1016/j.msec.2018.11.043

Cited by 29 publications

(25 citation statements)

References 55 publications

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“…The amide I (1700-1600 cm -1 ) peak with the wavenumber at 1632cm -1 originated from C=O stretching vibration. The amide II (1600-1500 cm -1 ) peaks which appeared around 1550cm -1 resulted from C-N stretching and N-H bending vibrations (Zhu, et al, 2019). The intensities of these peaks increased compared to those in free lipase.…”

Section: Ft-ir Spectra Studiesmentioning

confidence: 95%

The mechanism of chlorogenic acid inhibits lipid oxidation: An investigation using multi-spectroscopic methods and molecular docking

et al. 2020

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Endogenous lipase and lipoxygenase play important roles in accelerating lipid oxidation.Polyphenols are a series of commonly used chemicals for preserving fish and seafood products, due to their positive inhibitory effects on lipid oxidation. However, the mechanism involved is still unknown. The inhibitory effects of chlorogenic acid (CGA) on lipase and lipoxygenase were investigated and explored with multi-spectroscopic and molecular docking approaches. Results showed that CGA could inhibit the activities of lipase and lipoxygenase with concentration increased in a highly dose-dependent manner. CGA quenched intrinsic fluorescence intensities of enzymes by static quenching and binding with CGA which led to changes in 3D structures of enzymes. Results of the molecular docking confirmed binding modes, binding sites and major interaction forces between CGA and enzymes, which reduced the corresponding activity. Thus, this study could provide basic mechanisms of the inhibitory effects of polyphenols on lipid oxidation during food preservation.

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Section: Ft-ir Spectra Studiesmentioning

confidence: 95%

The mechanism of chlorogenic acid inhibits lipid oxidation: An investigation using multi-spectroscopic methods and molecular docking

et al. 2020

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“…The highest expression of hsp47 and type I collagen genes occurred in the heart and skin, while the highest expression of hsp47-like is higher in skin and swim bladder. Grass carp skin was reported to contain an abundance of type I collagen [7,14], which is a fibril-forming collagen usually consisting of two identical a1-chains and an a2-chain [2,6,24]. Hsp47 is essential in the process of collagen synthesis and it is strongly correlated with expression of type I collagen in multiple tissues [2,4].…”

Section: Discussionmentioning

confidence: 99%

“…At present, 29 different types of collagen have been found and named (types I-XXIX) and they play different roles in tissues. Type I collagen is the most abundant collagen in vertebrate tissues and is a major component of the extracellular matrix [6,7]. Type I collagen consists of three α chains and it has a central triple-helical domain containing Gly-X-X triplets covering more than 300 residues.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise

Liang

Wan

Shen

et al. 2021

Fishes

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Heat shock protein 47 (Hsp47) is a collagen-specific molecular chaperone that is indispensable for molecular maturation of collagen. In this study, hsp47 and hsp47-like cDNAs were cloned and characterized in grass carp (Ctenopharyngodon idella). The cDNAs were 1212 and 1218 base pairs long, respectively, and included an open reading frame encoding 403 and 405 amino acids. The molecular phylogeny based on the deduced amino acid sequences indicated that the correct sequences of the hsp47 and hsp47-like cDNA were obtained and the deduced proteins clustered distinctly into teleost clades. Primary structure analysis and characterization of Hsp47 and Hsp47-like shared the basic structure and biofunctions of Hsp47 in vertebrates. The spatial pattern of gene expression revealed that hsp47 and hsp47-like were relatively ubiquitous in different tissues and highly expressed in heart and skin. The expression levels of hsp47 and hsp47-like and type I collagen mRNAs varied similarly in different tissues. Type I collagen content increased significantly with the increase of water velocity in the muscle of grass carp in response to aerobic exercise. Among the gene expression changes of hsp47, hsp47-like, col1a1 and col1a2 in muscle that occurred in response to aerobic exercise, the change of type I collagen was most strongly correlated with hsp47 expression. Additionally, col1a1 showed the highest correlation with hsp47-like and col1a2 showed the highest correlation with hsp47. These findings suggest that grass carp Hsp47 and Hsp47-like are closely related to type I collagen synthesis. This study firstly suggests fish aerobic exercise can improve type I collagen content and Hsp47 gene expression in muscle of grass carp.

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“…Aunque hubo diferencia entre los muestreos, la biomasa del sitio MM resultó ser interesante por su alto contenido proteico a través de los diferentes meses de muestreo. Esto reafirma que los subproductos de origen pesquero pueden ser una buena fuente de proteína y MM, no es la excepción, quien además presentó H; por lo que, en base a su contenido, podría ser una alternativa para la extracción de biomoléculas como el colágeno y gelatina (Zhu et al, 2019;Vázquez et al, 2019).…”

Section: Discusión Primer Sitio De Muestreo (Mm)unclassified

“…Los llamados co-productos (hígado, molleja, cabeza, gónadas, esqueletos y descartes de maquillado) pueden consumirse sin procesar, ya que son considerados alimentos y por consecuencia, deben cumplir las mismas regulaciones y manejo (Jayathilakan et al, 2012). Los subproductos (vísceras, colas, pieles, exoesqueleto, escamas, etcétera), no son valorados como alimento directo para humanos; sin embargo, se están apreciando por sus biocomponentes de interés tecnológico, tales como: péptidos bioactivos (Rabiei et al, 2017, Vázquez et al, 2019, colágeno (Zhu et al, 2019), gelatina (Vázquez et al, 2019), hidroxiapatita (Komur et al, 2017), sulfato de condoitrina (He et al, 2017), ensilados (Özyurt et al, 2019) y otros con aplicaciones en la industria García-Sifuentes et al: Biotecnia / XXII (2): 61-69 (2020) alimentaria, farmacéutica, médica, biomédica, ingeniería ambiental, cosmética, entre otras (Siewe et al, 2019). Aunque los co-productos y subproductos son identificados de manera diferente, frecuentemente en los grandes centros de distribución o incluso en las mismas plantas de proceso, no hay una separación adecuada de los mismos, por lo que se generan y acopian en el mismo sitio mezclas de subproductos y co-productos.…”

Section: Introductionunclassified

Explorando diferentes subproductos considerados como residuos por la industria pesquera en México//Exploring different by-products considered as residues by the fishery industry in México

2020

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La producción y poco aprovechamiento de subproductos de los grandes centros de distribución en México causa problemas ambientales. Se exploró la composición fisicoquímica de distintos subproductos evaluando el pH, humedad, lípidos, ceniza, proteínas, nitrógeno no proteico, bases volátiles totales, hidroxiprolina y prolina en tres sitios (zonas) de muestreo durante tres meses diferentes, cada zona o sitio representó muestras diferentes, así: MM (mezcla de subproductos de diferentes especies), AT (subproductos derivados del enlatado de atún), TL (subproductos derivados del fileteo de tilapia). Se registraron diferencias en la composición química entre los tres meses de muestreo y entre los tres sitios evaluados. Los resultados mostraron que los componentes más importantes que se pueden recuperar son: proteínas, para el sitio MM, incluyendo el colágeno; lípidos y proteínas, para el sitio AT; colágeno y lípidos para el sitio TL. Los valores promedio de pH (7.89), humedad (72.75 %) y BVT (188 mg N/100 g) para el sitio MM, demuestran la necesidad de mejorar el manejo de los subproductos en ese sitio. Los subproductos de los tres sitios de muestreo mostraron ser una fuente alternativa de componentes importantes como proteínas que no son colágeno, colágeno, lípidos y cenizas, dependiendo del sitio. Sin embargo, es importante resaltar la necesidad de mejorar el manejo de los subproductos para lograr un mayor aprovechamiento.ABSTRACTThe production and under-utilization of by-products from the large distribution centers in México, promotes environmental problems. The physicochemical composition of different by-products produced by the large distribution centers throughout different months was explored evaluating pH, moisture, lipids, ash, proteins, non-protein nitrogen, total volatile bases, hydroxyproline and proline, in three places during three months, each place represented different sample, including: MM (by-products from different fish species), AT (by-products of tuna canning, and TL (tilapia by-products). There were differences in chemical composition among the three months and the evaluated places. Results showed that the most important components that could be recovered are proteins for MM, collagen, lipids and proteins for AT and collagen and lipids for TL. Average values of pH (7.89), moisture (72.75 %) and total volatile bases (188 mg N/100 g) demonstrate the need of improving the by-products management in the MM place. By-products from the three evaluated places showed be an alternative source to obtain important components including non-collagen proteins, collagen, lipids, and ash depending on the sampling place. However, it is important to highlight the need of improving the handling of by-products to achieve greater exploitation.

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A quantitative comparable study on multi-hierarchy conformation of acid and pepsin-solubilized collagens from the skin of grass carp (Ctenopharyngodon idella)

Cited by 29 publications

References 55 publications

The mechanism of chlorogenic acid inhibits lipid oxidation: An investigation using multi-spectroscopic methods and molecular docking

The mechanism of chlorogenic acid inhibits lipid oxidation: An investigation using multi-spectroscopic methods and molecular docking

Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise

Explorando diferentes subproductos considerados como residuos por la industria pesquera en México//Exploring different by-products considered as residues by the fishery industry in México

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