A quantitative assay for the number of chromophores on a chromoprotein; its application to phycoerythrin and phycocyanin

Brody, Seymour Steven; Brody, Michael

doi:10.1016/0006-3002(61)90333-x

Cited by 77 publications

(24 citation statements)

References 8 publications

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“…The phycocyanin extinction coefficient was obtained by passing a concentrated suspension of cells, buffered at pH 7'5 using 0.02 M Hepes, through a Yeda press five times at 100 kg cm -2 followed by centrifugation at 26,000 g for 30 min. The extract was decanted off and the pellet resuspended then the procedure repeated, with a final centrifugation of 1 h. The absor-bance of the two samples was read at 628 nm and the phycocyanin concentration estimated using an extinction coefficient of 7.9 (Brody & Brody, 1961). The mean in vivo extinction coefficient of phycocyanin for seven experiments was 7.76 (SD ±0.34).…”

Section: Experimental Methodsmentioning

confidence: 99%

Photosynthetic characteristics of planktonic blue-green algae: Changes in photosynthetic capacity and pigmentation ofOscillatoria redekeivan Goor under high and low light

Foy¹,

Gibson²

1982

British Phycological Journal

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Section: Experimental Methodsmentioning

confidence: 99%

Photosynthetic characteristics of planktonic blue-green algae: Changes in photosynthetic capacity and pigmentation ofOscillatoria redekeivan Goor under high and low light

Foy¹,

Gibson²

1982

British Phycological Journal

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“…It is conceivable that the acidic, radiolabeled, ninhydrin-negative substances are carboxymethyl-or carboxyethyl-pyrroledicarboxylic acid breakdown products of phycocvanobilin similar to those obtained from acid oxidation of biliverdin (11). In any event, significant quan- (4), and on establishing the molecular weight of the chromoprotein by sedimentation analysis in the uiltracentrifuge (3,8). Since the phycocyanin molecule consists of subunits which associate and dissociate reversibly with changes in pH and ionic strength of the buffer solution (12), and since phycocyanin is not homogeneous in the ultracentrifuge between pH 4.5 and 9.0 (1, 12), the "true" molecular weight of phycocyanin would appear to be an enigma.…”

mentioning

confidence: 99%

Formation, Chromophore Composition, and Labeling Specificity of Cyanidium caldarium Phycocyanin

Troxler

Lester

1968

Plant Physiol.

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“…The absorption spectrum of that water soluble pigment had a maxima at 312 nm and a shoulder peak at 330 nm. The pigment was functionally comparable to flavonoids of higher plants (CALDWELL et al 1983). An increase in the concentration of flavonoids has shown to protect higher plants from W -B damage (SEARLES et al 1995).…”

Section: Discussionmentioning

confidence: 97%

The response of three strains of Anacystis nidulans (cyanobacteria) to ultraviolet‐B radiation

Pandey

Chatterjee

1997

J. Basic Microbiol.

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The influnce of UV-B irradiance was examined on three strains of a cyanobacterium, Anacystis nidulam.These include wild type A. nidulans (An) and strains resistant to cadmium (Cd? and cyanophage As-1 (As-1"). The results indicated that although, the UV-B radiation considered in the present study was damaging to all the three strains, As-1' offered some degree of protection from UV-BLas far as changes in chlorophyll, phycocyanin, "CO, uptake and photosynthetic O2 evolution were concerned. Cadmium resistant strain appeared to be most sensitive to UV-B among the three. The observations form the first report of UV-B effects on a metal resistant and a cyanophage resistant strain of cyanobacterium.SAFFERMAN and M o w s (1963) discovered that the cyanophages could play important role in irradicating waterbloom forming cyanobacteria. Since then, cyanophages have successfully been deployed for cleaning water bodies from bloom forming cyanobacterial species. At the same time, however, some of the cyanophages were also found to kill economically important cyanobacterial strains. Since the in situ protection of such strains from these viruses could not possible, attempts were made to develop mutants of these organisms that could resist to their respective cyanophages (SINGH 1975, AMLA 1976, MISHRA et al. 1993, PANDEY and PANDEY 1994a, and 1994b. The question, however, that appears more interesting at this stage is that what would be the fate of such cyanophage resistant cyanobacterial strains when exposed to environmental stresses. Since the possibility of simultaneous Occurrence of a number of stress factors in aquatic systems cannot be ruled out, this could form an interesting part of scientific investigation.Among the several factors heavy metal pollution (VENKATARAMAN et al. 1992) and increasingly high level of W -B radiation reaching to the earth surface ( M m 1991), are the most important stress factors that received attention worldwide. The adverse effects of both, W -B (TERAMURA et al. 1990, SEARLES et al. 1995, MADRONICH 1995 and cadmium ( GREGER and ORGEN 1989, BORNMAN and DUBE 1991, JULES and SHAW 1994, PANDEY and PANDEY 1994aand 1994b

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A quantitative assay for the number of chromophores on a chromoprotein; its application to phycoerythrin and phycocyanin

Cited by 77 publications

References 8 publications

Photosynthetic characteristics of planktonic blue-green algae: Changes in photosynthetic capacity and pigmentation ofOscillatoria redekeivan Goor under high and low light

Photosynthetic characteristics of planktonic blue-green algae: Changes in photosynthetic capacity and pigmentation ofOscillatoria redekeivan Goor under high and low light

Formation, Chromophore Composition, and Labeling Specificity of Cyanidium caldarium Phycocyanin

The response of three strains of Anacystis nidulans (cyanobacteria) to ultraviolet‐B radiation

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