A quantitative approach to histopathological dissection of elastin-related disorders using multiphoton microscopy

Tong, Philip L.; Qin, Jim; Cooper, Caroline; Lowe, Patricia; Murrell, Dédée F.; Kossard, Steven; Ng, Laiguan; Roediger, Ben; Weninger, Wolfgang; Haass, Nikolas K.

doi:10.1111/bjd.12430

Cited by 28 publications

(29 citation statements)

References 36 publications

(127 reference statements)

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“…While the SHG signal in the dermis mainly originates from collagen I fibers and can be used for their quantification and morphology assessment 36 , intense TPEAF from this skin region is considered to be due to elastin fibers 37 . As a result, several descriptors have been developed to assess the morphological state of the upper dermis on the basis of MPT 37–39 , thus solving the principal quantification problem for dermatology and cosmetology. It was also shown that SHG microscopy itself is a powerful method, which allows obtaining detailed information on the collagen I fibers structure and their arrangement in tissues, providing for valuable clinical information 40–43 .…”

Section: Introductionmentioning

confidence: 99%

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Shirshin

Gurfinkel

Priezzhev

et al. 2017

Sci Rep

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The papillary dermis of human skin is responsible for its biomechanical properties and for supply of epidermis with chemicals. Dermis is mainly composed of structural protein molecules, including collagen and elastin, and contains blood capillaries. Connective tissue diseases, as well as cardiovascular complications have manifestations on the molecular level in the papillary dermis (e.g. alteration of collagen I and III content) and in the capillary structure. In this paper we assessed the molecular structure of internal and external regions of skin capillaries using two-photon fluorescence lifetime imaging (FLIM) of endogenous compounds. It was shown that the capillaries are characterized by a fast fluorescence decay, which is originated from red blood cells and blood plasma. Using the second harmonic generation signal, FLIM segmentation was performed, which provided for spatial localization and fluorescence decay parameters distribution of collagen I and elastin in the dermal papillae. It was demonstrated that the lifetime distribution was different for the inner area of dermal papillae around the capillary loop that was suggested to be due to collagen III. Hence, we propose a generalized approach to two-photon imaging of the papillary dermis components, which extends the capabilities of this technique in skin diagnosis.

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Section: Introductionmentioning

confidence: 99%

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Shirshin

Gurfinkel

Priezzhev

et al. 2017

Sci Rep

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“…Furthermore, there were two publications on MDE that did not report new clinical cases (El-Khoury et al, 2014, Tong et al, 2013). Finally, there was one report of two male patients with Type III MDE (Meyer et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Interstitial histiocytes, multinucleated giant cells, and occasional figures of elastophagocytosis have been reported (Neri et al, 1996). Interestingly, it has been observed that the degree of histological inflammation parallels the presence of elastophagocytosis (Patroi et al, 2003, Tong et al, 2013). …”

Section: Discussionmentioning

confidence: 99%

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Mid-dermal elastolysis: A female-centric disease; case report and updated review of the literature

Hardin

Dupuis

Haber

2015

International Journal of Women's Dermatology

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BackgroundMid-dermal elastolysis (MDE) is a rare, yet well-defined clinical and histopathologic entity manifested by fine wrinkling of the skin and mid-dermal loss of elastic fibers. This disease predominantly affects young to middle-aged Caucasian females and although it has no reported systemic features, it is psychologically bothersome and can be of great cosmetic concern.MethodsWe report a case of a healthy 45 year-old female with widespread mid-dermal elastolysis. A literature search using the search terms “mid-dermal elastolysis,” “mid dermal elastolysis,” “middermal elastolysis,” and “elastophagocytosis” was conducted on Pubmed, using articles published from January 2008 until November 2014 to accompany Gambichler’s comprehensive 1977 to 2009 review of mid-dermal elastolysis. The references of relevant papers were reviewed and further cases included as appropriate.ResultsWe review the clinical features and histological, ultrastructural, and immunohistochemical findings of MDE, as well as differential diagnoses. There are 13 new publications of MDE since 2008. The novel findings since Gambichler’s review are discussed and pathomechanisms revisited. Interestingly, given the striking female predominance of MDE, there is no known hormonal role in its etiology.

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“…Typical imaging methods such as confocal laser microscopy and multiphoton microscopy have been instrumental to exploit certain structures and molecules of the skin without the need for tissue processing[8–10]. Despite their high sensitivity and spatial resolution, it is almost impossible with them to make a globally chemical analysis in biological tissues.…”

Section: Introductionmentioning

confidence: 99%

Identification of Skin Electrical Injury Using Infrared Imaging: A Possible Complementary Tool for Histological Examination

Lin

et al. 2017

PLoS ONE

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In forensic practice, determination of electrocution as a cause of death usually depends on the conventional histological examination of electrical mark in the body skin, but the limitation of this method includes subjective bias by different forensic pathologists, especially for identifying suspicious electrical mark. The aim of our work is to introduce Fourier transform infrared (FTIR) spectroscopy in combination with chemometrics as a complementary tool for providing an relatively objective diagnosis. The results of principle component analysis (PCA) showed that there were significant differences of protein structural profile between electrical mark and normal skin in terms of α-helix, antiparallel β-sheet and β-sheet content. Then a partial least square (PLS) model was established based on this spectral dataset and used to discriminate electrical mark from normal skin areas in independent tissue sections as revealed by color-coded digital maps, making the visualization of electrical injury more intuitively. Our pilot study demonstrates the potential of FTIR spectroscopy as a complementary tool for diagnosis of electrical mark.

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A quantitative approach to histopathological dissection of elastin-related disorders using multiphoton microscopy

Abstract: MPM has the potential for noninvasive in vivo visualization of elastic fibres in the dermis with near histological resolution. DEMI allows objective assessment of elastic fibres to support diagnosis and monitoring of disease progress or therapy of elastin-related skin disorders.

Cited by 28 publications

References 36 publications

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Two-photon autofluorescence lifetime imaging of human skin papillary dermis in vivo: assessment of blood capillaries and structural proteins localization

Mid-dermal elastolysis: A female-centric disease; case report and updated review of the literature

Identification of Skin Electrical Injury Using Infrared Imaging: A Possible Complementary Tool for Histological Examination

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