A quantitative analysis of triglyceride droplet structural changes in hamster brown adipose tissue during cold exposure and starvation

Ahlabo, Iréne; Barnard, Tudor

doi:10.1016/s0022-5320(74)80006-7

Cited by 13 publications

(3 citation statements)

References 17 publications

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“…Thus, the multidroplet arrangement of triglyceride in the typical brown adipocyte may be considered to represent a more active disposition, metabolically, than is the case with the typical white adipocyte, in which a unilocular distribution is closely approached. In the BAT of adult hamsters exposed to a temperature of 4°C for 1 week, Ahlabo and Barnard (1974) noted a redistribution of lipid within brown adipocytes into smaller and more numerous droplets. This reorganization was estimated to result in a two-to threefold increase in lipid droplet surface density.…”

Section: Resultsmentioning

confidence: 95%

“…According to Ahlabo and Barnard (1974), it is reasonable to assume that in BAT as in white adipose tissue (WAT), hormone-sensitive lipase activity is predominantly located in the cytosol rather than bound to a specific membrane system. Therefore, the surface area of triglyceride exposed to cytoplasm could serve as an 2Differences10ne-way analysis of variance revealed significant differences (P < 0.005) among the age groups with regard to both S(1) and V(v).…”

Section: Resultsmentioning

confidence: 99%

“…It may well be that these smaller droplets compensate for the relatively low interfacial area contributed by the larger droplets to the total surface density of lipid within the tissue. It is thus probable that brown adipocytes are able to redistribute their lipid droplets into various size categories in order to maintain the surface density of the triglyceride store during maturation and aging, a phenomenon comparable to the multilocular reorganization observed by Ahlabo and Barnard (1974) in the brown adipocytes of cold-exposed hamsters.…”

Section: S(1): (B) Vs (E)mentioning

confidence: 86%

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Stereological assessment of age‐related changes in lipid droplet surface area and vascular volume in rat interscapular brown adipose tissue

Nnodim

1988

Anat. Rec.

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The surface area of stored triglyceride and the volume of capillaries in the interscapular brown fat pad of the rat have been adopted as morphological indices of the overall thermogenic capability of the tissue. The present study examines the relationship between the chronological profiles of these parameters and reported senile changes in the biochemical and physiological characteristics of brown adipose tissue. Lipid droplet surface density and vascular volume density were estimated by computer-assisted planimetry with electron micrographs prepared from interscapular brown adipose tissue samples obtained from rats of various ages. The volumes of the fat pad at these ages were also determined and used to calculate droplet surface areas and vascular volumes. Triglyceride surface area showed a 20-fold enhancement in the early postnatal period (0.44 X 10(2) cm2 at birth; 9.43 X 10(2) cm2 at 4 weeks). A further but less remarkable increase occurred in adulthood (11.67 X 10(2) cm2 at 6 months) and, in old age, only a slight fall was noted (9.66 X 10(2) cm2 at 2 years). Intralobular capillary volume also rose sharply early during the first week after birth, reaching a peak at 4 weeks (2.40 X 10(-2) cm3). The values recorded in adulthood and old age (1.90 X 10(-2) cm3 at 6 months; 1.76 X 10(-2) cm3 at 2 years) were not significantly different from that obtained at 4 weeks of age. These results show that the attainment of maximum values of lipid droplet surface area and vascular volume in rat interscapular brown adipose tissue coincides with the period of the tissue's peak metabolic capacity.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: S(1): (B) Vs (E)mentioning

confidence: 86%

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