A quantitative analysis of the biogenic amines in the central ganglia of the pond snail, Lymnaea stagnalis (L.)

Hetherington, M S; McKenzie, Jessamyn; Dean, H. G.; Winlow, William

doi:10.1016/1367-8280(94)90014-0

Cited by 10 publications

(8 citation statements)

References 31 publications

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“…It is well known that the molluscan immune system is based on innate mechanisms, whereas humoral and cellular processes approve cytotoxic and antimicrobial roles (Auffret, Rousseau, Boutet, Tanguy, & Baron, 2006). Outcomes of the present study is in agreement with former studies of biogenic amines in freshwater gastropods (for example, Hetherington, McKenzie, Dean, & Winlow, 1994;Ottaviani, Caselgrandi, Petraglia, & Franceschi, 1992). Chiang, Bourgeois, and Beuding (1974) separated and identified dopamine and 5-HT in numerous tissues of Balanus glabrata, plus the ganglia, cephalopedal area, and mantle.…”

Section: Discussionsupporting

confidence: 81%

Biological investigations on the freshwater snail Pirenella conica (Blainville, 1829) infected with the developmental stages of Heterophyes sp.

Rizk

HAMADA

Abd-ElGhany

et al. 2018

JoBAZ

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Background: Heterophyiasis is an intestinal sickness promoted by infection with the heterophyid digenetic worms. It is one of the major infectious diseases of public health in the developing countries. Method: Single-cell gel eletrophoresis, or comet assay, was carried out for detecting DNA damage in the digestive gland cells of Pirenella conica infected with Heterophyes larvae. Besides, apoptosis, some isoenzymes, and two biogenic amines (neurotransmitters) were investigated using the flow cytometric analysis, the starch gel electrophoresis, and the HPLC techniques respectively. Snails were collected from brackish water area around Port Said province during the spring-summer periods of 2012-2013. Results:The results showed that infection with the larval trematodes increased tail length (length of DNA migration) in the digestive gland cells of infected snails. Meanwhile, the percentage of apoptosis was significantly elevated (58.80%) in the snails infected with the larval trematodes as compared to that of uninfected snails (39. 59%). Apparent polymorphism was detected in the four enzymes obtained from the digestive gland extracts.

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Section: Discussionsupporting

confidence: 81%

Biological investigations on the freshwater snail Pirenella conica (Blainville, 1829) infected with the developmental stages of Heterophyes sp.

Rizk

HAMADA

Abd-ElGhany

et al. 2018

JoBAZ

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“…Hence, total protein secretion by the AG consists primarily of HdAGP. Other neurotransmitters commonly found in the CNS of molluscs, including serotonin, acetylcholine, norepinephrine, GABA, histamine, octopamine and glutamate (Audesirk, 1985;Hetherington et al, 1994), had no effect on AG protein secretion, suggesting that the effect of dopamine on the AG was specific. Indeed, dopamine was the only neurotransmitter detected in significant quantity in the AG of H. duryi by HPLC (Kiehn et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…The catecholamine dopamine has a widespread distribution in the molluscan central nervous system (CNS; Hetherington et al, 1994;Trimble et al, 1984;Audesirk, 1985;Muneoka et al, 1991), where it is a neurotransmitter regulating numerous physiological processes including learning and memory (Lukowiak and Syed, 1999), the regulation of neurite outgrowth and synaptic connectivity (Spencer et al, 1998), embryonic neural development (Croll, 2000) and the regulation of neural circuits involved in feeding (Elliott and Susswein, 2002) and respiration (Taylor and Lukowiak, 2000). Dopamine is also found in peripheral tissues of molluscs, where it controls physiological processes such as smooth muscle contraction (Gies, 1986), motility of the gastrointestinal tract (Hernadi et al, 1998) and salivary secretion (Kiss et al, 2003).…”

mentioning

confidence: 99%

Dopamine stimulates snail albumen gland glycoprotein secretion through the activation of a D1-like receptor

Mukai

Kiehn

Saleuddin

2004

Journal of Experimental Biology

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The catecholamine dopamine is present in both the central nervous system and in the peripheral tissues of molluscs, where it is involved in regulating reproduction. Application of exogenous dopamine to the isolated albumen gland of the freshwater pulmonate snail Helisoma duryi (Wetherby) induces the secretion (release) of perivitelline fluid. The major protein component of the perivitelline fluid of Helisoma duryi is a native 288·kDa glycoprotein that is secreted around individual eggs and serves as an important source of nutrients for the developing embryos. The secretion of glycoprotein by the albumen gland is a highly regulated event that must be coordinated with the arrival of the fertilized ovum at the carrefour (the region where the eggs receive albumen gland secretory products). In order to elucidate the intracellular signalling pathway(s) mediating dopamineinduced glycoprotein secretion, albumen gland cAMP production and glycoprotein secretion were measured in the presence/absence of selected dopamine receptor agonists and antagonists. Dopamine D1-selective agonists dihydrexidine, 6,7-ADTN and SKF81297 stimulated cAMP production and glycoprotein secretion from isolated albumen glands whereas D1-selective antagonists SCH23390 and SKF83566 suppressed dopaminestimulated cAMP production. Dopamine D2-selective agonists and antagonists generally had no effect on cAMP production or protein secretion. Based on the effects of these compounds, a pharmacological profile was obtained that strongly suggests the presence of a dopamine D1-like receptor in the albumen gland of Helisoma duryi. In addition, secretion of albumen gland glycoprotein was not inhibited by protein kinase A inhibitors, suggesting that dopamine-stimulated protein secretion might occur through a protein kinase A-independent pathway.

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“…The cerebral and buccal ganglia, which also have numerous serotonergic neurons, have more 5-HT than the buccal and pleural ganglia, which lack any serotonergic cell bodies. In Lymnaea stagnalis, a freshwater gastropod, the pedal ganglia also have the highest amount of 5-HT (McCaman et al, 1984; Hetherington et al, 1994) and have the greatest number of 5-HT containing neurons (Kemenes et al, 1989). Thus, the HPLC measurements of 5-HT amounts can be used as a rough estimate of the number of serotonergic neurons in the central ganglia of these species.…”

Section: Hplc Measurements Of Amine Levelsmentioning

confidence: 99%

Localization and quantification of 5‐hydroxytryptophan and serotonin in the central nervous systems of Tritonia and Aplysia

et al. 2001

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Serotonin (5-hydroxytryptamine, 5-HT) plays a central role in several behaviors in marine molluscs and other species. In an effort to better understand the regulation of 5-HT synthesis, we used high performance liquid chromatography (HPLC) with electrochemical detection and immunohistochemistry to measure and map the distribution of the immediate precursor of 5-HT, 5-hydroxytryptophan (5-HTP), in two model opisthobranch molluscs, the nudibranch Tritonia diomedea and the anaspid Aplysia californica. HPLC measurements showed that 5-HTP is present at approximately the same level as the 5-HT metabolite, 5-hydroxyindolacetic acid (5-HIAA) but is more than 100 times lower in concentration than either 5-HT or dopamine in the same tissue. Specific 5-HTP immunoreactivity was colocalized with serotonin in both species. The overall intensity of 5-HTP immunoreactivity in individual ganglia agreed with HPLC measurements for those ganglia. The intensity of 5-HTP immunolabeling varied between cell types and was correlated with the intensity of 5-HT immunolabeling. In particular, differences in staining intensity were consistently seen among the three dorsal swim interneurons of the Tritonia swim central pattern generator circuit. Some nonserotonergic neurons also displayed low levels of 5-HTP immunolabeling that were above background levels. Together, these results support the notion that production of 5-HTP is a rate-limiting step in serotonin synthesis and suggest that there may be additional regulation that allows 5-HTP to accumulate to varying levels.

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A quantitative analysis of the biogenic amines in the central ganglia of the pond snail, Lymnaea stagnalis (L.)

Cited by 10 publications

References 31 publications

Biological investigations on the freshwater snail Pirenella conica (Blainville, 1829) infected with the developmental stages of Heterophyes sp.

Biological investigations on the freshwater snail Pirenella conica (Blainville, 1829) infected with the developmental stages of Heterophyes sp.

Dopamine stimulates snail albumen gland glycoprotein secretion through the activation of a D1-like receptor

Localization and quantification of 5‐hydroxytryptophan and serotonin in the central nervous systems of Tritonia and Aplysia

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