A quantitation of movement of marker particles in the plasma membrane of 3T3 mouse fibroblasts

Albrecht-Bühler, G.; Yarnell, Margaret M.

doi:10.1016/0014-4827(73)90037-2

Cited by 12 publications

(6 citation statements)

References 3 publications

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“…10). Microparticles so conveyed are somehow adherent to the plasma membrane (29,28,6,2), an attachment the tenacity of which may be dependent on a local reorganization of the underlying and adjacent microfilaments system, like that postulated by Allison (7) and demonstrated in macrophages by Reaven and Axline (49). The base of the cortical site underlying attached macromarkers appears in a conformation indicative of spreading or stretching (49), but the edges may be locally contracted (7,66,67).…”

Section: Movement Of Zeiotic Knobs and Of Markers At The Cell Surfacementioning

confidence: 93%

“…At first hemispherical, they subsequently enlarge and develop clavate or bulbous ends on erect, narrow stalks, thus assuming the form of knobs (Figs. 5,6). These migrate on the cell surface, first coming together in small peripheral clusters which move centripetally and coalesce into larger groups Figs.…”

Section: Overviewmentioning

confidence: 99%

“…x 66,000. or microtubules are but rarely discerned (Figs. [5][6][7][8]. The terminal bulb contains endoplasm represented by dispersed monoribosomes and some bodies of <10 nm, interpretable as ribosomal subunits.…”

Section: ~0mentioning

confidence: 99%

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Action of cytochalasin D on cells of established lines. III. Zeiosis and movements at the cell surface.

Godman¹,

Miranda²,

Deitch³

et al. 1975

The Journal of Cell Biology

136

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The projection of knobby protuberances at the cell surface (zeiosis) is a general cellular response to cytochalasin D (CD), resulting from herniation of endoplasm through undefended places of the cortex during cell contractions and displacement of microfilaments induced by CD. Zeiosis is prevented by agents that interfere with the contractile response to CD, such as inhibitors of energy metabolism or cyclic AMP. The developed protrusions, which remain relatively stable in the presence of CD, contain chiefly mono-or subribosomes, and occasionally other organelles normally resident in endoplasm; compact microfilament felt occupies their bases and extends into their proximal stalks. Protein synthesis in the knobs is less than half of that in the polyribosome-containing endoplasm residual in the main body of the cell.Knobs first protrude singly near the margin of the contracting cells and rapidly cluster into small groups in the periphery even at lower temperature. The clusters then migrate centripetally and coalesce into a single large aggregate near the apex of the immobilized and retracted cell: this movement is energy-and temperaturedependent. Aggregation is more prominent and stable in cell lines of epithelial derivation than in fibroblastic or other lines in which nuclear extrusion occurs more readily. The latter is regarded as a special manifestation of zeiosis.Macromarkers, such as latex spherules, migrate like the zeiotic knobs on the cell surfaces in the presence of CD. The aggregated knobs, although persistent for days in the presence of CD, are rapidly recessed after withdrawal of the agent as ruffling is resumed and the cells spread. These movements are discussed in terms of current concepts of mobility of the cell membrane.Most arresting among the visible effects of the tion oflocomotory and membrane movements (12, cytochalasins on cells in monolayer are the cessa-65), the expulsion of their nuclei 12), and the 644

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Section: Movement Of Zeiotic Knobs and Of Markers At The Cell Surfacementioning

confidence: 93%

Section: Overviewmentioning

confidence: 99%

See 1 more Smart Citation

Action of cytochalasin D on cells of established lines. III. Zeiosis and movements at the cell surface.

Godman¹,

Miranda²,

Deitch³

et al. 1975

The Journal of Cell Biology

136

View full text Add to dashboard Cite

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“…The particles around the cells are mostly cleaned out by surface protrusions during the first hour after plating and become partly internalised (phagocytosed) and partly accumulated on the cell surface in big clumps without being phagocytosed (AlbrechtBuehler and Lancaster 1976). Therefore, the movement of such cells reflects a surface movement rather than a cytoplasmic movement (Albrecht-Buehler and Yarnell 1973). In order to distinguish locomotion on plain surfaces from this combination of phagocytosis and cellular displacement, we better indicate this phenomenon as "phagokinetics" and the particle-free tracks conveniently visualised as "phagokinetic tracks".…”

Section: Locomotion Assaymentioning

confidence: 99%

Cytoskeleton changes and impaired motility of monocytes at modelled low gravity

et al. 2006

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Summary. Investigations performed in space have shown that gravity changes affect important cellular mechanisms like proliferation, differentiation, genetic expression, cytoskeletal architecture, and motility in lymphocytes, monocytes, and other mammalian cells. In particular, a dramatic depression of the mitogenic in vitro activation of human peripheral blood lymphocytes was observed at low gravity. The hypothesis of the present work is that a reduced interaction between T lymphocytes and monocytes, essential for the second signalling pathway, might be one of the reasons for the observed depression of the in vitro activation of human lymphocytes. Cell motility and with it a continuous rearrangement of the cytoskeletal network within the cell is essential for cell-tocell contacts. Whereas nonactivated lymphocytes in suspension are highly motile at low gravity, no data are available so far on the motility of adherent monocytes. It thus can be argued that impaired monocyte locomotion and cytoskeletal changes could be responsible for a reduced interaction of monocytes with T lymphocytes. In this study, the locomotion ability of J-111 cells, an adherent monocyte cell line, attached to colloidal gold particles on coverslips and exposed to modelled low gravity in the random positioning machine was found to be severely reduced compared with that of controls and the structures of actin, tubulin, and vinculin were affected.

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“…Previous studies have shown that the average diameter of gold particles formed in this manner is approximately 400 nm. 19 In order to verify the final surface particle concentration, transmitted microscopy images of the substrate surface were obtained at 1003 magnification and analyzed using image analysis. In experiments where no gold particulates were used, substrates were coated with a solution made without AuCl 4 H.…”

Section: Substrate Preparatio Nmentioning

confidence: 99%

Regulation of Cell Motility on Polymer Substrates via "Dynamic," Cell Internalizable, Ligand Microinterfaces

Tjia

Moghe

2002

Tissue Engineering

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In vivo, motile epidermal tissues frequently encounter ligand microinterfaces that are dynamic, owing to rapid cell-mediated substrate phagocytosis. In this study, we have examined cell motility phenomena in response to the adhesion ligand, collagen, which was presented on cell-internalizable 400-nm colloidal gold microcarriers. Normal human keratinocytes were seeded onto collagen-adsorbed poly(lactide-co-glycolide) (PLGA) films that were predeposited with varying densities of the ligand-associated microcarriers (LAMs), such that the overall ligand density and the ligand loading per microcarrier were invariant. Cells seeded on LAMs exhibited rapid and distinct cytoskeletal redistribution resulting in numerous filopodial extensions, indicative of activation of cell motility processes. We report that the population-averaged cell migration rate of cells, mu, was increased from 2 microm2/min on collagen substrates lacking the microcarriers, to approximately 50 microm2/min on collagen presenting LAMs. An increase in LAM density to 1.2 LAMs per microm2 was found to maximize mu, as well as the directional persistence and speed of cell migration, whereas very high LAM densities saturated cell internalization and diminished migration rates. The cell-LAM interactions essential to enhanced migration were ligand-activated, as mu; values were reduced 400% on internalizable microcarriers lacking ligands; moreover, cooperative ligand elicitation was possible, for example, when 10 microg/mL soluble fibronectin was introduced as a costimulant of keratinocytes, yielding the highest reported mu values (80 microm2/min) on collagen LAM-PLGA substrates. Notably, cell migration rates were severely repressed when cell internalization processes were challenged through covalent conjugation of ligand carriers to the substrate, indicating that signals from cell-LAM binding alone were inadequate for elevated levels of cell migration. Further analysis indicated that the presence of LAMs did not alter the protease-resistant adhesivity between the cell and the underlying substrate, suggesting that the activation governing ligand interactions likely arose at the cell-LAM interface rather than the cell-substrate interface. This study highlights the novel use of secondary ligand-presenting microscale/nanoscale depots at polymer substrates to elicit, via dynamic cell internalization processes, significantly enhanced levels of cell migration over traditional interfaces with ligand-bearing substrates.

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A quantitation of movement of marker particles in the plasma membrane of 3T3 mouse fibroblasts

Cited by 12 publications

References 3 publications

Action of cytochalasin D on cells of established lines. III. Zeiosis and movements at the cell surface.

Action of cytochalasin D on cells of established lines. III. Zeiosis and movements at the cell surface.

Cytoskeleton changes and impaired motility of monocytes at modelled low gravity

Regulation of Cell Motility on Polymer Substrates via "Dynamic," Cell Internalizable, Ligand Microinterfaces

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