Abstract:A framework for reversed-phase HPLC method development was developed by combining computer simulation, UPLC scouting experiments and surface response design. An interactive computer simulation program was developed for optimizing the gradient elution conditions. This simulation program integrated the kinetics and flow resistance parameters of 18 commonly used columns. Accurate prediction of retention times by this simulation program was demonstrated in this work. This program was used to propose the optimum gr… Show more
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