A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in <i>Cannabis sativa</i> L.

Fischedick, Justin T.; Glas, Ronald; Hazekamp, Arno; Verpoorte, Robert

doi:10.1002/pca.1143

Cited by 51 publications

(31 citation statements)

References 15 publications

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“…Several other HPLC methods were also developed for the determination of cannabinoids [15–18]. All of these methods, however, are not employed for the determination of CB13 in a possible pharmaceutical dosage form and usually employed to quantify illicit substances in biological fluids.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Álvarez-Fuentes

Martín-Banderas

Muñoz-Rubio

et al. 2012

The Scientific World Journal

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A simple, fast, and reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for determining of a cannabinoid derivate, which displays potent antihyperalgesic activity, 1-naphthalenyl[4-(pentyloxy)-1-naphthalenyl]methanone (CB13) into PLGA nanoparticles. Separation was achieved in a C18 column using a mobile phase consisting of two solvents: solvent A, consisting of acetonitrile : water : acetic acid (75 : 23.7 : 1.3 v/v), and solvent B, consisting of acetonitrile. An isocratic method (70 : 30 v/v), with a flow rate of 1.000 mL/min, and a diode array detector were used. The developed method was precise, accurate, and linear over the concentration range of analysis with a limit of detection and a limit of quantification of 0.5 and 1.25 μg/mL, respectively. The developed method was applied to the analysis of CB13 in nanoparticles samples obtained by three different procedures (SEV, FF, and NPP) in terms of encapsulation efficiency and drug release. Nanoparticles size and size distribution were also evaluated founding that NPP method presented the most lowest particle sizes with narrow-size distribution (≈320 nm) and slightly negative zeta potential (≈−25 mV) which presumes a suitable procedure for the synthesis of PLGA-CB13 nanoparticles for oral administration.

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Section: Introductionmentioning

confidence: 99%

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Álvarez-Fuentes

Martín-Banderas

Muñoz-Rubio

et al. 2012

The Scientific World Journal

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“…Indeed, both the FDA and EMA guidelines for method validation require assessment and reduction for suppression of ionisation in MS-based analytical procedures [75, 76]. Structural analogues can be used to mitigate ionisation efficiency as well as matrix-induced ion suppression [53] or enhancement [54]. However, they may lack sufficient structural similarity to coelute and provide full compensation.…”

Section: Validation Characteristics For Analytical Proceduresmentioning

confidence: 99%

“…Specificity has consequences for identification and differentiation between compounds having close homology, as well as for purity testing and for quantitative determination [49]. Not all analytical procedures across the manufacturing pipeline require a high level of specificity (complete discrimination) [32, 54], providing they can be compensated by one or more supporting analytical procedure(s) [49]. For example, thin layer chromatography is suitable for quantitative and semi-quantitative assessment of cannabinoids within botanical raw material [32, 54].…”

Section: Validation Characteristics For Analytical Proceduresmentioning

confidence: 99%

“…Not all analytical procedures across the manufacturing pipeline require a high level of specificity (complete discrimination) [32, 54], providing they can be compensated by one or more supporting analytical procedure(s) [49]. For example, thin layer chromatography is suitable for quantitative and semi-quantitative assessment of cannabinoids within botanical raw material [32, 54]. However, more discriminatory procedures, which include targeted analysis on a larger portfolio of cannabinoids as well as interfering materials, are typically used during purification of drug forms in the manufacturing pipeline [31] (Fig.…”

Section: Validation Characteristics For Analytical Proceduresmentioning

confidence: 99%

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Developing Robust Standardised Analytical Procedures for Cannabinoid Quantification: Laying the Foundations for an Emerging Cannabis-Based Pharmaceutical Industry

Welling¹,

Liu²,

Hazekamp³

et al. 2019

Med Cannabis Cannabinoids

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The plant genus Cannabis is a prolific producer of unique pharmaceutically relevant metabolites, commonly referred to as cannabinoids. Robust and standardised methods for the quantification of cannabinoids within botanical and drug forms is a critical step forward for an emerging Cannabis-based pharmaceutical industry, which is poised for rapid expansion. Despite a growing body of analytical methods for the quantification of cannabinoids, few have been validated using internationally accredited guidelines. Moreover, standardised methods have yet to be developed for application at various stages of manufacture as well as for different levels of processing and refinement. Validation parameters for establishing robust standardised methods for cannabinoid quantification within Cannabis-based drug forms are critically discussed. Determining an appropriate level of specificity (discrimination) among heterogeneous botanical matrices as well as evaluating accuracy (recovery) and inter-laboratory precision (reproducibility) within strict and volatile regulatory environments are potential obstacles to the establishment of robust analytical procedures. We argue that while some of these challenges remain unique to Cannabis, others are common to botanical-based drug development and manufacture. In order to address potential barriers to analytical method standardisation, a collaborative research initiative inclusive of academic and commercial stakeholders is proposed.

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“…The analysis involves an immunoassay, GC-MS and LC-MS for the confirmation and quantification of the drugs. [9][10][11][12][13][14][15][16][17][18][19][20][21][22] The immunochemical method is generally used as a preliminary analysis because of its evident advantage in rapidity. However, the main drawback is the low selectivity of the target compound.…”

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confidence: 99%

A Reliable Method for the Separation and Detection of Synthetic Cannabinoids by Supercritical Fluid Chromatography with Mass Spectrometry, and Its Application to Plant Products

Toyo’oka

Kikura‐Hanajiri²

2015

CHEMICAL & PHARMACEUTICAL BULLETIN

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A reliable method using supercritical fluid chromatography with mass spectrometry (SFC-MS) was developed for cannabinoids using compressed carbon dioxide (CO 2 ) and methanol as the mobile-phase. The cannabinoids, i.e., cannabicyclohexanol ( , were determined within 12 min using a conventional column (2-EP) for SFC. Furthermore, two optical isomers of CCH and trans-CCH were completely and rapidly separated by a chiral stationary phase column (AMY1). A highly sensitive detection (0.002-3.75 ppb) was also obtained by these methods using 2-EP and AMY1 columns. These methods were applied to the qualitative and quantitative determination of cannabinoids in dried plant products. Although the concentration and species were different in the products, JWH-018, JWH-073 and CCH, including the cis-isomer, trans-isomer and the optical isomers, were detected in the products. Therefore, the proposed SFC-MS method seems to be useful as an alternative method to GC-MS and LC-MS for illegal drugs, such as cannabinoids.

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A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L.

Abstract: Results have shown that the HPTLC method is reproducible and accurate for the quantification of Delta(9)-THC in cannabis. The method is also useful for the qualitative screening of the main neutral cannabinoids found in cannabis cultivars.

Cited by 51 publications

References 15 publications

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Developing Robust Standardised Analytical Procedures for Cannabinoid Quantification: Laying the Foundations for an Emerging Cannabis-Based Pharmaceutical Industry

A Reliable Method for the Separation and Detection of Synthetic Cannabinoids by Supercritical Fluid Chromatography with Mass Spectrometry, and Its Application to Plant Products

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