A pyrosequencing-based method to quantify genetic substitutions associated with resistance to succinate dehydrogenase inhibitor fungicides in <i>Botrytis</i>
 spp. populations

Gobeil-Richard, Mélanie; Tremblay, D. M.; Beaulieu, Carole; Heyden, Hervé Van der; Carisse, Odile

doi:10.1002/ps.4026

Cited by 25 publications

(18 citation statements)

References 32 publications

(93 reference statements)

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“…Pesticides, especially fungicides, are important for protecting crops and increasing yields in agriculture . However, fungicide resistance has evolved in recent years . Rice is one of the world's major and oldest food crops.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and in vivo fungicidal activity of some new quinoline derivatives against rice blast

Liu

Fang

Xie

et al. 2017

Pest Management Science

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Section: Introductionmentioning

confidence: 99%

“…1 However, fungicide resistance has evolved in recent years. 2 Rice is one of the world's major and oldest food crops. Rice blast, Pyricularia oryae, is the most economical important rice disease worldwide.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and in vivo fungicidal activity of some new quinoline derivatives against rice blast

Liu

Fang

Xie

et al. 2017

Pest Management Science

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“…However, it has been proposed that HRM analysis can be combined with qPCR in case that quantitative data are required (Rouleau et al, 2009). Currently, the only available method for quantitative assessment of sdh B mutants in B. cinerea is a recently developed pyrosequencing-based method (Gobeil-Richard et al, 2015). A third disadvantage of the method that has to be taken into account, when it is applied in fungicide resistance monitoring programs, is the probability for the presence of natural polymorphisms in the amplified products that might change the melting profile of the amplicon leading to false results.…”

Section: Discussionmentioning

confidence: 99%

“…The above mentioned techniques require the fungal isolation, while multiple reactions are necessary to identify the mutations associated with the resistance. In addition to the above mentioned techniques, a newer method, that of pyrosequencing, was developed offering the advantage of quantification of several sdh B mutations in B. cinerea populations (Gobeil-Richard et al, 2015)…”

Section: Introductionmentioning

confidence: 99%

Detection of sdhB Gene Mutations in SDHI-Resistant Isolates of Botrytis cinerea Using High Resolution Melting (HRM) Analysis

2016

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Botrytis cinerea, is a high risk pathogen for fungicide resistance development. Pathogen’ resistance to SDHIs is associated with several mutations in sdh gene. The diversity of mutations and their differential effect on cross-resistance patterns among SDHIs and the fitness of resistant strains necessitate the availability of a tool for their rapid identification. This study was initiated to develop and validate a high-resolution melting (HRM) analysis for the identification of P225H/F/L//T, N230I, and H272L/R/Y mutations. Based on the sequence of sdhB subunit of resistant and sensitive isolates, a universal primer pair was designed. The specificity of the HRM analysis primers was verified to ensure against the cross-reaction with other fungal species and its sensitivity was evaluated using concentrations of known amounts of mutant’s DNA. The melting curve analysis generated nine distinct curve profiles, enabling the discrimination of all the four mutations located at codon 225, the N230I mutation, the three mutations located in codon 272, and the non-mutated isolates (isolates of wild-type sensitivity). Similar results were obtained when DNA was extracted directly from artificially inoculated strawberry fruit. The method was validated by monitoring the presence of sdhB mutations in samples of naturally infected strawberry fruits and stone fruit rootstock seedling plants showing damping-off symptoms. HRM analysis data were compared with a standard PIRA–PCR technique and an absolute agreement was observed suggesting that in both populations the H272R mutation was the predominant one, while H272Y, N230I, and P225H were detected in lower frequencies. The results of the study suggest that HRM analysis can be a useful tool for sensate, accurate, and rapid identification of several sdhB mutations in B. cinerea and it is expected to contribute in routine fungicide resistance monitoring or assessments of the effectiveness of anti-resistance strategies implemented in crops heavily treated with botryticides.

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“…Fungicidal agents are important and effective pesticides in crop protection . Because of the long‐term use of antifungal agents, resistance has increased in recent years . There is therefore a need to discover new highly active compounds with high effectiveness, low toxicity and that are environmentally friendly, and which also may overcome resistance problems .…”

Section: Introductionmentioning

confidence: 99%

Novel 4‐pyrazole carboxamide derivatives containing flexible chain motif: design, synthesis and antifungal activity

Liu

Zhai

et al. 2019

Pest Management Science

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Background In recent years, carboxamide fungicides, targeting succinate dehydrogenase (SDH), have shown highly efficient and broad spectrum fungicidal activity. Structure–activity relationship (SAR) results for these commercial fungicides show that the carboxamide group was a key active group. This is useful information for the discovery of new pyrazole carboxamide derivatives with fungicidal activity. Results Twenty‐seven novel pyrazole carboxamides were designed and synthesized. Their fungicidal activities against Gibberella zeae, Phytophthora infestans, Phytophthora capsici, Rhizoctonia solani, Alternaria solani, Botrytis cinerea, Fusarium oxysporum, Cercospora arachidicola, Sclerotinia sclerotiorum and Physalospora piricola were tested; derivatives possessed excellent inhibitory at 50 mg L−1 in particular. Furthermore, some pyrazole carboxamides exhibited remarkably high activities against Sclerotinia sclerotiorum in vitro with EC50 values of 2.04 to 15.2 μg mL−1. In addition, some compounds also exhibited high activities against Physalospora piricola, Cercospora arachidicola and Phytophthora capsici. Inhibition activities against SDH proved that the designed analogues were effective at the enzyme level. The SAR of these pyrazole carboxamides was studied by using the docking method. Conclusion It is possible that pyrazole carboxamides, which exhibit good activity against Sclerotinia sclerotiorum, can be further optimized as a lead compounds of carboxamide fungicides. © 2019 Society of Chemical Industry

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A pyrosequencing-based method to quantify genetic substitutions associated with resistance to succinate dehydrogenase inhibitor fungicides in Botrytis spp. populations

Cited by 25 publications

References 32 publications

Synthesis and in vivo fungicidal activity of some new quinoline derivatives against rice blast

Synthesis and in vivo fungicidal activity of some new quinoline derivatives against rice blast

Detection of sdhB Gene Mutations in SDHI-Resistant Isolates of Botrytis cinerea Using High Resolution Melting (HRM) Analysis

Novel 4‐pyrazole carboxamide derivatives containing flexible chain motif: design, synthesis and antifungal activity

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