A pyrophosphatase activity associated with purified HIV-1 particles

Ducloux, Céline; Mougel, Marylène; Goldschmidt, Valérie; Didierlaurent, Ludovic; Marquet, Roland; Isel, Catherine

doi:10.1016/j.biochi.2012.06.025

Cited by 1 publication

(1 citation statement)

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“…The above in vitro experiments provide insight into the material properties of virus cores, but it is important to understand how these attributes are relevant and what role they play during the actual infection process in vivo. The increase in stiffness can be attributed to the presence of rigid nucleic acid within the capsid, but could also be due to the generation of free pyrophosphates (PPi) that phosphorylate the inner wall of multiple virus capsids, causing minor structural changes [ 70 , 71 , 72 , 73 ]. Similarly, infectivity studies have shown an increase in the stiffness or stability of the virus core due to point mutations; however, this increase does not necessarily correlate with reduced infectivity in all cases [ 22 , 57 ].…”

Section: The Mechanics Of Virus Uncoatingmentioning

confidence: 99%

Applications of Atomic Force Microscopy in HIV-1 Research

Rousso

Deshpande

2022

Viruses

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Obtaining an understanding of the mechanism underlying the interrelations between the structure and function of HIV-1 is of pivotal importance. In previous decades, this mechanism was addressed extensively in a variety of studies using conventional approaches. More recently, atomic force microscopy, which is a relatively new technique with unique capabilities, has been utilized to study HIV-1 biology. Atomic force microscopy can generate high-resolution images at the nanometer-scale and analyze the mechanical properties of individual HIV-1 virions, virus components (e.g., capsids), and infected live cells under near-physiological environments. This review describes the working principles and various imaging and analysis modes of atomic force microscopy, and elaborates on its distinctive contributions to HIV-1 research in areas such as mechanobiology and the physics of infection.

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