A putative TRAPPII tethering factor is required for cell plate assembly during cytokinesis in <i>Arabidopsis</i>

Jaber, Emad; Thiele, Knut; Kindzierski, Viktoria; Loderer, Christoph; Rybak, Katarzyna; Jürgens, Gerd; Mayer, Ulríke; Söllner, Rosemarie; Wanner, Gerhard; Assaad, Farhah F.

doi:10.1111/j.1469-8137.2010.03331.x

Cited by 38 publications

(50 citation statements)

References 71 publications

(192 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Colocalization at the TGN and functional studies suggest that TRAPPII acts as a GEF for RABA1c, facilitating RABA1c-mediated trafficking of material from the TGN to the cell plate (Qi et al, 2011). Mutants of the TRAPPII subunit, AtTRS130, exhibit severe cytokinesis defects and irregular aggregations of RABA1c around cell plate-like structures (Jaber et al, 2010;Qi et al, 2011). The expression of constitutively active RABA1c partially rescues attrs130 (Qi et al, 2011), supporting the role of AtTRS130 upstream of RABA1c.…”

Section: Tgn and The Cell Plate: A Timely Connectionmentioning

confidence: 89%

The Plant Trans-Golgi Network: Not Just a Matter of Distinction

2017

View full text Add to dashboard Cite

Section: Tgn and The Cell Plate: A Timely Connectionmentioning

confidence: 89%

The Plant Trans-Golgi Network: Not Just a Matter of Distinction

2017

View full text Add to dashboard Cite

“…An insertion mutant in the TRAPPII gene Trs130 was found to be allelic to club-1 (Sö llner et al, 2002); therefore, we named it trs130/club-2. club mutants, which were identified as being cytokinesis defective in a forward genetic screen, are described elsewhere (Jaber et al, 2010). In this study, we characterize the Arabidopsis Trs120 ortholog (AtTRS120).…”

Section: Screening Insertion Mutants In Arabidopsis Tethering Factorsmentioning

confidence: 99%

Tethering Factors Required for Cytokinesis in Arabidopsis

et al. 2010

Self Cite

View full text Add to dashboard Cite

At the end of the cell cycle, the nascent cross wall is laid down within a transient membrane compartment referred to as the cell plate. Tethering factors, which act by capturing vesicles and holding them in the vicinity of their target membranes, are likely to play an important role in the first stages of cell plate assembly. Factors required for cell plate biogenesis, however, remain to be identified. In this study, we used a reverse genetic screen to isolate tethering factors required for cytokinesis in Arabidopsis (Arabidopsis thaliana). We focused on the TRAPPI and TRAPPII (for transport protein particle) tethering complexes, which are thought to be required for the flow of traffic through the Golgi and for trans-Golgi network function, as well as on the GARP complex, thought to be required for the tethering of endocytotic vesicles to the trans-Golgi network. We found weak cytokinesis defects in some TRAPPI mutants and strong cytokinesis defects in all the TRAPPII lines we surveyed. Indeed, four insertion lines at the TRAPPII locus AtTRS120 had canonical cytokinesis-defective seedling-lethal phenotypes, including cell wall stubs and incomplete cross walls. Confocal and electron microscopy showed that in trs120 mutants, vesicles accumulated at the equator of dividing cells yet failed to assemble into a cell plate. This shows that AtTRS120 is required for cell plate biogenesis. In contrast to the TRAPP complexes, we found no conclusive evidence for cytokinesis defects in seven GARP insertion lines. We discuss the implications of these findings for the origin and identity of cell plate membranes.

show abstract

“…5b, c), similar to vesicles frequently associated with the nascent cell plate in freshly divided cells. However, it is interesting to note that none of the cells with protuberances were enlarged or were multi-nucleated, the typical morphological characteristics of cytokinesis mutants (Jaber et al 2010). It looked as if the protuberances occurred randomly as a misdirected layering of cell wall material on the inner surface of the existing primary cell wall.…”

Section: Resultsmentioning

confidence: 99%

“…In our study, cell wall defects were found only in cells with a single nucleus which was predominantly in interphase. In many cytokinesis mutants, the reported cell wall stubs were situated in the equatorial division plane attached to the parental wall at opposite ends, sometimes leaving a gap in the middle, suggesting that cell plate formation, expansion, or maturation, had failed at some point (Assaad et al 1996;Jaber et al 2010;Lukowitz et al 1996). In contrast, cell wall ingrowths in sac9 cells arose at random sites from the preexisting wall growing towards the center of the cell, but not restricted to the position of a future cell plate.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, if cytokinesis had been affected in sac9 embryos, seedling lethality might be expected. Many cytokinesis mutants do not survive the embryo stage (Assaad et al 1996;Jaber et al 2010;Liu et al 1995;Lukowitz et al 1996;Strompen et al 2002) or are merely conditional mutants (Eleftheriou et al 2005). However, sac9 seeds germinated and seedlings successfully established, ruling out a primary defect in cytokinesis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Unique cell wall abnormalities in the putative phosphoinositide phosphatase mutant AtSAC9

Vollmer

Youssef

DeWald

2011

Planta

View full text Add to dashboard Cite

SAC9 is a putative phosphoinositide phosphatase in Arabidopsis thaliana involved in phosphoinositide signaling. sac9-1 plants have a constitutively stressed phenotype with shorter roots which notably accumulate phosphatidylinositol 4,5-bisphosphate and its hydrolysis product inositol trisphosphate. We investigated the primary roots of sac9-1 seedlings at the cytological and ultrastructural level to determine the structural basis for this altered growth. Despite the normal appearance of organelles and cytoplasmic elements, our studies reveal extreme abnormalities of cell wall and membrane structures in sac9-1 primary root cells, regardless of cell type, position within the meristematic area, and plane of section. Cell wall material was deposited locally and in a range of abnormal shapes, sometimes completely fragmenting the cell. Simple protuberances, broad flanges, diffuse patches, elaborate folds, irregular loops and other complex three-dimensional structures were found to extend randomly from the pre-existing cell wall. Abundant vesicles and excessive membrane material were associated with these irregular wall structures. We argue that a perturbed phosphoinositide metabolism most likely induces these observed abnormalities and hypothesize that a disorganized cytoskeleton and excessive membrane trafficking mediate the cell wall defects.

show abstract

A putative TRAPPII tethering factor is required for cell plate assembly during cytokinesis in Arabidopsis

Cited by 38 publications

References 71 publications

The Plant Trans-Golgi Network: Not Just a Matter of Distinction

The Plant Trans-Golgi Network: Not Just a Matter of Distinction

Tethering Factors Required for Cytokinesis in Arabidopsis

Unique cell wall abnormalities in the putative phosphoinositide phosphatase mutant AtSAC9

Contact Info

Product

Resources

About