A putative transcription factor Gf.BMR1 in Grifola frondosa, the homolog of BMR1 in Bipolaris oryzae, was strongly induced by near-ultraviolet light and blue light

Kurahashi, Atsushi; Shimoda, Takafumi; Sato, Masayuki; Fujimori, Fumihiro; Hirama, Junji; Nishibori, Kozo

doi:10.1016/j.myc.2014.05.005

Cited by 11 publications

(9 citation statements)

References 22 publications

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“…A blast search of PKS63787 performed in NCBI GenBank identified a gene (BAO20284.1; E value = 0) encoding a PKS (GfPKS) in Grifola f rondosa, a member of Polyporales with potent medicinal uses. 27,28 Quantitative reverse transcription PCR (qRT-PCR) analysis indicated that pks63787 was continuously expressed in different growing periods on potato dextrose agar (PDA) (Figure S1). The annotated transcriptome also revealed general expression of pks63787 in monokaryotic A. cinnamomea strains of different mating types (unpublished data), suggesting that PKS63787related metabolites may be present universally in various strains.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

pks63787, a Polyketide Synthase Gene Responsible for the Biosynthesis of Benzenoids in the Medicinal Mushroom Antrodia cinnamomea

Yu,

Chang,

Liou

et al. 2016

J. Nat. Prod.

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Antrodia cinnamomea, a unique resupinate basidiomycete endemic to Taiwan, has potent medicinal activities. The reddish basidiocarps and mycelia generally exhibit abundant metabolites and higher biological activity. To investigate the pigments of A. cinnamomea, polyketide synthase (PKS) genes were characterized based on its partially deciphered genome and the construction of a fosmid library. Furthermore, a gene disruption platform was established via protoplast transformation and homologous recombination. Of four putative polyketide synthase genes, pks63787 was selected and disrupted in the monokaryotic wild-type (wt) strain f101. Transformant Δpks63787 was deficient in the synthesis of several aromatic metabolites, including five benzenoids and two benzoquinone derivatives. Based on these results, a biosynthetic pathway for benzenoid derivatives was proposed. The pks63787 deletion mutant not only displayed a reduced red phenotype compared to the wt strain but also displayed less 1,1-biphenyl-2-picrylhydrazyl free radical scavenging activity. This finding suggests that PKS63787 is responsible for the biosynthesis of pigments and metabolites related to the antioxidant activity of A. cinnamomea. The present study focuses on the functional characterization of the PKS gene, the fluctuations of its profile of secondary metabolites, and interpretation of the biosynthesis of benzenoids.

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Section: ■ Results and Discussionmentioning

confidence: 99%

pks63787, a Polyketide Synthase Gene Responsible for the Biosynthesis of Benzenoids in the Medicinal Mushroom Antrodia cinnamomea

Yu,

Chang,

Liou

et al. 2016

J. Nat. Prod.

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“…The transcription factor Gf . BMR1 has been proved to be induced by near-ultraviolet and blue light and regulate the fruiting body formation . In Bipolaris oryzae , BMR1 regulated 3 melanin biosynthesis genes .…”

Section: Discussionmentioning

confidence: 99%

“…BMR1 has been proved to be induced by near-ultraviolet and blue light and regulate the fruiting body formation. 52 In Bipolaris oryzae, BMR1 regulated 3 melanin biosynthesis genes. 53 Several transcription factors involved in secondary metabolite biosynthesis or fruiting body formation have been identified in the putative genetic network.…”

Section: ■ Discussionmentioning

confidence: 99%

Differential Gene Expression Network in Terpenoid Synthesis ofAntrodia cinnamomeain Mycelia and Fruiting Bodies

Lin

Lee

et al. 2017

J. Agric. Food Chem.

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Antodia cinnamomea, a precious brown-rot fungus endemic to Taiwan, has pharmaceutical applications due to its diverse array of metabolites. The terpenoids found in A. cinnamomea contribute to its most important bioactivities. We identified several terpenoid compounds in A. cinnamomea and revealed that their content in mycelium and fruiting body were significantly different. Using next-generation sequencing and an in-house transcriptome database, we identified several terpene synthase (TPS) candidates. After sequence analysis and functional characterization, 10 out of 12 candidates were found to have single or multiple terpene synthesis functions. Most of the terpenoid compounds were found to confer important bioactivities. RT-PCR results showed a positive correlation between terpene synthase expression pattern and terpenoid content. In addition, we identified several modification enzyme candidates that may be involved in the postmodification of terpenoid compounds with a genomic DNA scaffold, and a putative genetic network.

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“…For example, undesirable growth (e.g. cloudy mycelia color, reduced pileus growth) was observed in several mushrooms such as, Grifola frondosa, Coprinus cinereus, and Hypsizygus marmoreus, under dark culture conditions [15][16][17][18][19]. Shiratori et al [19] further revealed that the color of mycelia was regulated by the amount and quality of light.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the edible mushroom Lentinula edodes in response to blue light

Kim

Park

et al. 2020

PLoS ONE

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Lentinula edodes is one of the most popular edible mushrooms worldwide and contains important medicinal components such as lentinan, ergosterol, and eritadenine. Mushroom metabolism is regulated by the mycelia and fruit body using light; however, in mushrooms, the underlying molecular mechanisms controlling this process as well as light-induced gene expression remain unclear. Therefore, in this study, we compared morphological changes and gene expression in the fruit bodies of L. edodes cultivated under blue light and continuous darkness. Our results showed that blue light primarily induced pileus growth (diameter and thickness) compared to dark cultivation. Alternatively, stipe length development was promoted by dark cultivation. We also performed RNAseq on L. edodes under the blue light/ dark cultivation conditions. A total of 12,051 genes were used for aligning the Illumina raw reads and 762 genes that showed fold change cut-offs of >|2| and significance p-values of <0.05 were selected under blue light condition. Among the genes which showed two-fold changed genes, 221 were upregulated and 541 were downregulated. In order to identify blue light induced candidate genes, differentially expressed genes (DEGs) were selected according to 4-fold changes and validated by RT-PCR. We identified 8 upregulated genes under blue light condition, such as DDR48-heat shock protein, Fasciclin-domain-containing protein and carbohydrate esterase family 4 protein, FAD NAD-binding domain-containing protein that are involved in morphological development of primordium and embryonic muscle development, cell adhesion and affect the structure of cellulosic and non-cellulosic cell walls of fruit body development, and photoreceptor of blue light signaling for fruit body and pigment development, respectively. This study provides valuable insights into the molecular mechanisms underlying the role of blue light in mushroom growth and development and can thus contribute to breeding programs to improve mushroom cultivation.

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A putative transcription factor Gf.BMR1 in Grifola frondosa, the homolog of BMR1 in Bipolaris oryzae, was strongly induced by near-ultraviolet light and blue light

Cited by 11 publications

References 22 publications

pks63787, a Polyketide Synthase Gene Responsible for the Biosynthesis of Benzenoids in the Medicinal Mushroom Antrodia cinnamomea

pks63787, a Polyketide Synthase Gene Responsible for the Biosynthesis of Benzenoids in the Medicinal Mushroom Antrodia cinnamomea

Differential Gene Expression Network in Terpenoid Synthesis ofAntrodia cinnamomeain Mycelia and Fruiting Bodies

Transcriptome analysis of the edible mushroom Lentinula edodes in response to blue light

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