A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated.

Donson, Jonathan; Morris-Krsinich, Bret A.M.; Mullineaux, Phil; Boulton, Margaret I.; Davies, Julian

doi:10.1002/j.1460-2075.1984.tb02260.x

Cited by 87 publications

(39 citation statements)

References 22 publications

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“…In addition, (31). This 3' end mapped 9bp from the 3' end of the major species of "primer-like" DNA molecules found on virion DNA (34). A polypeptide of 28,000 MW was mapped, by hybrid-arrested translation, to the region coding for the two-virion sense transcripts on MSV DNA (Fig.…”

Section: Discussionmentioning

confidence: 95%

Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene

et al. 1985

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Three RNA transcripts encoded by maize streak virus DNA were detected in polyadenylated RNA from virus-infected maize leaves.Two of the transcripts, a major 0.9kb and a minor 1.05kb RNA, were mapped on the virion (+) sense DNA and the other minor transcript of 1.2kb was mapped on the complementary (-) sense DNA, demonstrating that transcription of MSV DNA was bidirectional.The two virion sense transcripts were 3' coterminal at nucleotide 1114 but had 5' termini at nucleotides 2682 and 163 respectively. Virus-specific polyadenylated RNA translated in vitro to produce a 28,000MW polypeptide, specifically immunoprecipitable by antiserum raised against whole virus. The mRNA for this protein was mapped by hybrid-arrested translation to the long open reading frame in virion sense DNA whose potential amino acid composition, calculated from nucleotide sequence data, closely agreed with that determined experimentally for the coat protein.

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Section: Discussionmentioning

confidence: 95%

Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene

et al. 1985

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“…First, the monocot-type DNAs have two non-coding (intcrgenic) regions, whereas dicot-type DNAs have only one intergenic region in DNA 1. Second, the monocot-type viruses have small (approximately 80 nucleotides) complementary-strand DNAs which contain a few covalently bound, 5'-terminal ribonucleotides and are associated with and probably base-paired to viral DNA inside nucleocapsids (Howell, 1984;Donson et al, 1984Donson et al, , 1987Andersen et al, 1988;Hayes et al, 1988;Lazarowitz, 1988). These small DNAs are thought to be primers of complementary strand DNA synthesis.…”

Section: Comparison Of Coat Proteinsmentioning

confidence: 99%

Phylogeny of Geminiviruses

Howarth

Vandemark²

1989

Journal of General Virology

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SUMMARYAmino acid sequences of 16 geminivirus replication-associated proteins and 15 coat proteins were aligned and a new computer program was used to calculate the minimum mutation distances for all possible pairwise comparisons. These data were used to construct phylogenetic trees. Trees based on coat proteins had two main branches which were positively correlated with vector specificities of the viruses. Trees based on replication-associated proteins also had two main branches which were positively correlated with viral host specificities for either monocotyledonous or dicotyledonous plants. Therefore, evolutionary pressures on coat proteins and replication-associated proteins are probably highly influenced by vectors and hosts, respectively. Geminiviruses that infect dicotyledonous plants may be divided further by geographical origins into Old World and New World viruses. These results suggest the possible geographical origins of some geminiviruses, that new taxa should be erected, and have implications for distinguishing viruses and strains.

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“…It is likely that complementarysense DNA synthesis on this replicative intermediate proceeds by a similar mechanism to that on ssDNA following uncoating. The encapsidated viral DNA of geminiviruses that infect monocotyledonous plants is predominantly single-stranded but is associated with a complementary-sense DNA fragment of approximately 80 nucleotides containing 5' terminal ribonucleotides [6][7][8][9]. The ability to synthesise full-length dsDNA in vitro using viral DNA extracted from purified virus particles suggested that the fragment might serve as a primer for at least the first round of DNA synthesis in vivo.…”

Section: Introductionmentioning

confidence: 99%

RNA-primed complementary-sense DNA synthesis of the geminivirus African cassava mosaic virus

Saunders¹,

Lucy²,

Stanley³

1992

Nucl Acids Res

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The plant DNA virus African cassava mosaic virus (ACMV) is believed to replicate by a rolling circle mechanism. To investigate complementary-sense DNA (lagging strand) synthesis, we have analysed the heterogenous form of complementary-sense DNA (H3 DNA) from infected Nicotiana benthamiana by twodimensional agarose gel electrophoresis and blot hybridisation. The presence of an RNA moeity is demonstrated by comparison of results for nucleic acids resolved on neutral/alkaline and neutral/ formamide gels, suggesting that complementary-sense DNA synthesis on the virus-sense single-stranded DNA template is preceded by the synthesis of an RNA primer. Hybridisation with probes to specific parts of ACMV DNA A genome indicates that synthesis of the putative RNA primer initiates between nucleotides 2581 -221, a region that includes intergenic sequences that have been implicated in geminivirus DNA replication and the control of gene expression.

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A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated.

Cited by 87 publications

References 22 publications

Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene

Bidirectional transcription of maize streak virus DNA and identification of the coat protein gene

Phylogeny of Geminiviruses

RNA-primed complementary-sense DNA synthesis of the geminivirus African cassava mosaic virus

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