A Puf RNA-binding protein encoding gene PlM90 regulates the sexual and asexual life stages of the litchi downy blight pathogen Peronophythora litchii

Jiang, Liqun; Ye, Wenwu; Situ, Junjian; Chen, Yubin; Yang, Xinyu; Kong, Guanghui; Liu, Yaya; Tinashe, Runyanga J.; Xi, Pinggen; Wang, Yuanchao; Jiang, Zide

doi:10.1016/j.fgb.2016.12.002

Cited by 28 publications

(32 citation statements)

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“…To explore the biological functions of PlMAPK10 in P. litchii development, stress response, and pathogenicity, we generated an antisense direction of PlMAPK10 coding region, driven by the oomycete constitutive pHAM34 promoter in a pTOR vector ( Whisson et al, 2007 ) and transformed it into WT P. litchii protoplast cells following a well-established protocol ( Jiang et al, 2017 ). Transformation with pTOR vector served as blank control and named as CK strain.…”

Section: Resultsmentioning

confidence: 99%

“…Peronophythora litchii strain was isolated from a litchi fruit with litchi downy blight disease in Guangdong province, and purified by single-spore isolation using MSM400 Dissection Microscope (Singer Instruments) ( Jiang et al, 2017 ). The strain was cultured on carrot juice agar (CJA) media (juice from 200 g carrot topped up to 1 L, 15 g/L agar for solid media) at 25°C in the dark.…”

Section: Methodsmentioning

confidence: 99%

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PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection

et al. 2018

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Mitogen-activated protein kinase (MAPK) pathways are ubiquitous and evolutionarily conserved signal transduction modules directing cellular respond to a diverse array of stimuli, in the eukaryotic organisms. In this study, PlMAPK10 was identified to encode a MAPK in Peronophythora litchii, the oomycete pathogen causing litchi downy blight disease. PlMAPK10, containing a specific and highly conserved dual phosphorylation lip sequence SEY (Serine-Glutamic-Tyrosine), represents a novel group of MAPKs as previously reported. Transcriptional profiling showed that PlMAPK10 expression was up-regulated in zoospore and cyst stages. To elucidate its function, the PlMAPK10 gene was silenced by stable transformation. PlMAPK10 silence did not impair oospore production, sporangium germination, zoospore encyst, or cyst germination but hindered hyphal growth, sporulation, pathogenicity, likely due to altering laccase activity. Over all, our results indicated that a MAPK encoded by PlMAPK10 gene in P. litchii is important for pathogenic development.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection

et al. 2018

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“…MG197994) with those available in NCBI website. The oomycete pathogen P. litchii was cultured on potato dextrose agar (PDA) at 25 °C [ 35 ]. For production of volatile substances, BWL-H1 was cultured in potato sugar broth at 28 °C, shaking at 180 rpm for 5–7 d. Then, a spore suspension of BWL-H1 diluted from broth culture to the concentration of 1 × 10 7 spores/mL was inoculated on autoclaved wheat seeds in conical flasks (1000 mL), at 1 mL per 100 g of wheat seeds.…”

Section: Methodsmentioning

confidence: 99%

Antifungal Activity of Natural Volatile Organic Compounds against Litchi Downy Blight Pathogen Peronophythora litchii

et al. 2018

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Litchi (Litchi chinensis Sonn.) is a commercially important fruit but its production and quality are restricted by litchi downy blight, caused by the oomycete pathogen Peronophythora litchii Chen. Volatile substances produced by a biocontrol antinomycetes Streptomyces fimicarius BWL-H1 could inhibited P. litchii growth and development both in vitro and in detached litchi leaf and fruit infection assay. Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM) analyses indicated that volatile organic compounds (VOCs) from BWL-H1 resulted in severe damage to the endomembrane system and cell wall of P. litchii cells in vitro and abnormal morphology of appressoria, as well as deformed new hyphae in infection process. VOCs could suppress mycelial growth, sporulation, while with no obvious effect on sporangia germination. Based on gas chromatography-mass spectrophotometric analyses, 32 VOCs were identified from S. fimicarius BWL-H1, the most abundant of which was phenylethyl alcohol. Eight VOCs, including phenylethyl alcohol, ethyl phenylacetate, methyl anthranilate, α-copaene, caryophyllene, humulene, methyl salicylate and 4-ethylphenol, that are commercially available, were purchased and their bioactivity was tested individually. Except for humulene, the other seven tested volatile compounds shown strong inhibitory activity against mycelial growth, sporulation, sporangia germination and germ-tube growth of P. litchii. Especially, 4-ethylphenol showed the highest inhibitory effect on sporulation at a very low concentration of 2 µL/L. Overall, our results provided a better understanding of the mode of action of volatiles from BWL-H1 on P. litchii, and showed that volatiles from BWL-H1 have the potential for control of postharvest litchi downy blight.

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“…However, fewer studies have been conducted on the functions of P. litchii genes, hence there is scarcity of information about its pathogenesis and the litchi– P . litchii interaction mechanisms (Jiang et al , , ; Kong et al ., ). The identification and/or investigation of RXLR effectors in P. litchii lags behind that for other Phytophthora and downy mildew species, with only bioinformatics prediction of 245 putative RXLR effector genes (Ye et al , ).…”

Section: Introductionmentioning

confidence: 99%

An RXLR effector PlAvh142 from Peronophythora litchii triggers plant cell death and contributes to virulence

Situ

Jiang

Fan

et al. 2020

Molecular Plant Pathology

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Litchi downy blight, caused by the phytopathogenic oomycete Peronophythora litchii, results in tremendous economic loss in litchi production every year. To successfully colonize the host cell, Phytophthora species secret hundreds of RXLR effectors that interfere with plant immunity and facilitate the infection process. Previous work has already predicted 245 candidate RXLR effector‐encoding genes in P. litchii, 212 of which have been cloned and tested for plant cell death‐inducing activity in this study. We found three such RXLR effectors could trigger plant cell death through transient expression in Nicotiana benthamiana. Further experiments demonstrated that PlAvh142 could induce cell death and immune responses in several plants. We also found that PlAvh142 localized in both the cytoplasm and nucleus of plant cells. The cytoplasmic localization was critical for its cell death‐inducing activity. Moreover, deletion either of the two internal repeats in PlAvh142 abolished the cell death‐inducing activity. Virus‐induced gene silencing assays showed that cell death triggered by PlAvh142 was dependent on the plant transduction components RAR1 (require for Mla12 resistance), SGT1 (suppressor of the G2 allele of skp1) and HSP90 (heat shock protein 90). Finally, knockout of PlAvh142 resulted in significantly attenuated P. litchii virulence on litchi plants, whereas the PlAvh142‐overexpressed mutants were more aggressive. These data indicated that PlAvh142 could be recognized in plant cytoplasm and is an important virulence RXLR effector of P. litchii.

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A Puf RNA-binding protein encoding gene PlM90 regulates the sexual and asexual life stages of the litchi downy blight pathogen Peronophythora litchii

Cited by 28 publications

References 28 publications

PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection

PlMAPK10, a Mitogen-Activated Protein Kinase (MAPK) in Peronophythora litchii, Is Required for Mycelial Growth, Sporulation, Laccase Activity, and Plant Infection

Antifungal Activity of Natural Volatile Organic Compounds against Litchi Downy Blight Pathogen Peronophythora litchii

An RXLR effector PlAvh142 from Peronophythora litchii triggers plant cell death and contributes to virulence

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