A psychophysical and electrophysiological analysis of salt taste in <i>Trpv1</i> null mice

Treesukosol, Yada; Lyall, Vijay; Heck, Gerard L.; DeSimone, John A.; Spector, Alan C.

doi:10.1152/ajpregu.00587.2006

Cited by 76 publications

(54 citation statements)

References 39 publications

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“…This such as CAP and piperine, are received by the transient receptor potential cation channels known as subfamily V, member 1 (TRPV1) (Tominaga et al, 1998;Okumura et al, 2010). TRPV1 variant is reported as a candidate molecule for the salt receptor (Lyall et al, 2004;Treesukosol et al, 2007). These receptors may affect the enhancement of salt taste intensity by CAP.…”

Section: Discussionmentioning

confidence: 99%

Effect of Capsaicin on Salt Taste Sensitivity in Humans

Narukawa

Sasaki

Watanabe

2011

FSTR

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In order to investigate the effect of pungent components on salt taste sensitivity in humans, sensory evaluation of taste intensity and palatability was performed by adding 0.5 or 1 μM capsaicin (CAP) to a 75 mM NaCl solution. The assessment results suggested that a significant number of subjects experienced stronger salt taste intensity with the CAP-added NaCl solution than that for the NaCl solution alone. A quantitative analysis performed by adding CAP to the NaCl solution revealed an increase in the salt taste intensity. At the same time, the palatability decreased with increasing concentration of CAP. The increase in salt taste intensity after the addition of CAP suggests that addition of pungent components might influence salt sensitivity in humans.

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Section: Discussionmentioning

confidence: 99%

Effect of Capsaicin on Salt Taste Sensitivity in Humans

Narukawa

Sasaki

Watanabe

2011

FSTR

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“…Control stimuli consisting of 300 mM NH 4Cl and 300 mM NaCl applied at the beginning and end of the experiment were used to assess preparation stability (31,32). As in our previous studies, the control responses did not differ by more than 2-5% at the beginning and end of the experiment (11,48). The stimulus series used in the CT experiments to generate the relationship between RTX concentration and the magnitude of the NaCl ϩ Bz CT response was as follows: R ¡ 300 mM NH 4Cl ¡ R ¡ 300 mM NaCl ¡ R ¡ 100 mM NaCl ¡ R ¡ (100 mM NaCl…”

Section: Methodsmentioning

confidence: 99%

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

Ren

Rhyu

Phan

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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Ren Z, Rhyu M, Phan TT, Mummalaneni S, Murthy KS, Grider JR, DeSimone JA, Lyall V. TRPM5-dependent amilorideand benzamil-insensitive NaCl chorda tympani taste nerve response. Am J Physiol Gastrointest Liver Physiol 305: G106 -G117, 2013. First published May 2, 2013; doi:10.1152/ajpgi.00053.2013.-Transient receptor potential (TRP) subfamily M member 5 (TRPM5) cation channel is involved in sensing sweet, bitter, umami, and fat taste stimuli, complex-tasting divalent salts, and temperature-induced changes in sweet taste. To investigate if the amiloride-and benzamil (Bz)-insensitive NaCl chorda tympani (CT) taste nerve response is also regulated in part by TRPM5, CT responses to 100 mM NaCl ϩ 5 M Bz (NaCl ϩ Bz) were monitored in Sprague-Dawley rats, wild-type (WT) mice, and TRP vanilloid subfamily member 1 (TRPV1) and TRPM5 knockout (KO) mice in the presence of resiniferatoxin (RTX), a TRPV1 agonist. In rats, NaCl ϩ Bz ϩ RTX CT responses were also monitored in the presence of triphenylphosphine oxide, a specific TRPM5 blocker, and capsazepine and N-(3-methoxyphenyl)-4-chlorocinnamid (SB-366791), specific TRPV1 blockers. In rats and WT mice, RTX produced biphasic effects on the NaCl ϩ Bz CT response, enhancing the response at 0.5-1 M and inhibiting it at Ͼ1 M. The NaCl ϩ Bz ϩ SB-366791 CT response in rats and WT mice and the NaCl ϩ Bz CT response in TRPV1 KO mice were inhibited to baseline level and were RTX-insensitive. In rats, blocking TRPV1 by capsazepine or TRPM5 by triphenylphosphine oxide inhibited the tonic NaCl ϩ Bz CT response and shifted the relationship between RTX concentration and the magnitude of the tonic CT response to higher RTX concentrations. TRPM5 KO mice elicited no constitutive NaCl ϩ Bz tonic CT response. The relationship between RTX concentration and the magnitude of the tonic NaCl ϩ Bz CT response was significantly attenuated and shifted to higher RTX concentrations. The results suggest that pharmacological or genetic alteration of TRPM5 activity modulates the Bz-insensitive NaCl CT response and its modulation by TRPV1 agonists. triphenylphosphine oxide; resiniferatoxin; capsazepine; SB-366791; TRPV1 APPETITIVE AND AVERSIVE NEURAL and behavioral responses to NaCl are most likely transduced by a variety of mechanisms (35). Several studies suggest that in the anterior tongue Na ϩ from a Na ϩ salt taste stimulus enters a subset of taste bud cells by at least two types of cation channels located in taste cell apical membranes. One channel type is the Na ϩ -specific epithelial Na ϩ channel (ENaC), in which Na ϩ transport is inhibited by amiloride and benzamil (Bz) (1, 3, 8). The second involves Na ϩ transport through a putative transient receptor potential (TRP) vanilloid subfamily member 1 (TRPV1t)-nonspecific cation channel (26,27). The TRPV1t channel is insensitive to amiloride and Bz, permeable to Na ϩ , K ϩ , NH 4 ϩ , and Ca 2ϩ , and contributes to cell depolarization (31). These conclusions are supported by the observations that the phasic (transient) and tonic (steady-state) components of ...

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“…These mice have a targeted mutation caused by a disrupted exon encoding part of the fifth and all of the sixth transmembrane pore regions; age-matched C57 mice, which we obtained from Charles River Laboratories are the appropriate control mice (Caterina et al, 2000;Ciura and Bourque, 2006;Treesukosol et al, 2007). Mice were maintained in a 12 h light/dark cycle with standard rodent chow available ad libitum.…”

Section: Animals the Vanderbilt University Medical Center Institutiomentioning

confidence: 99%

“…8). Phosphorylation promotes sensitization, continued translocation, and increased depolarization (Van Buren et al, 2005). TRPV1 can become desensitized by dephosphorylation via the calmodulin-dependent protein phosphatase calcineurin or by reinternalization (Mohapatra and Nau, 2005).…”

Section: Trpv1mentioning

confidence: 99%

Absence of Transient Receptor Potential Vanilloid-1 Accelerates Stress-Induced Axonopathy in the Optic Projection

et al. 2014

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How neurons respond to stress in degenerative disease is of fundamental importance for identifying mechanisms of progression and new therapeutic targets. Members of the transient receptor potential (TRP) family of cation-selective ion channels are candidates for mediating stress signals, since different subunits transduce a variety of stimuli relevant in both normal and pathogenic physiology. We addressed this possibility for the TRP vanilloid-1 (TRPV1) subunit by comparing how the optic projection of Trpv1 Ϫ / Ϫ mice and age-matched C57 controls responds to stress from elevated ocular pressure, the critical stressor in the most common optic neuropathy, glaucoma. Over a 5 week period of elevated pressure induced by microbead occlusion of ocular fluid, Trpv1Ϫ / Ϫ accelerated both degradation of axonal transport from retinal ganglion cells to the superior colliculus and degeneration of the axons themselves in the optic nerve. Ganglion cell body loss, which is normally later in progression, occurred in nasal sectors of Trpv1 Ϫ / Ϫ but not C57 retina. Pharmacological antagonism of TRPV1 in rats similarly accelerated ganglion cell axonopathy. Elevated ocular pressure resulted in differences in spontaneous firing rate and action potential threshold current in Trpv1 Ϫ / Ϫ ganglion cells compared with C57. In the absence of elevated pressure, ganglion cells in the two strains had similar firing patterns. Based on these data, we propose that TRPV1 may help neurons respond to disease-relevant stressors by enhancing activity necessary for axonal signaling.

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A psychophysical and electrophysiological analysis of salt taste in Trpv1 null mice

Cited by 76 publications

References 39 publications

Effect of Capsaicin on Salt Taste Sensitivity in Humans

Effect of Capsaicin on Salt Taste Sensitivity in Humans

TRPM5-dependent amiloride- and benzamil-insensitive NaCl chorda tympani taste nerve response

Absence of Transient Receptor Potential Vanilloid-1 Accelerates Stress-Induced Axonopathy in the Optic Projection

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