A Provisional Classification of Cytoplasmic Polyhedrosis Viruses Based on the Sizes of the RNA Genome Segments

Payne, C. C.; Rivers, C. F.

doi:10.1099/0022-1317-33-1-71

Cited by 82 publications

(72 citation statements)

References 20 publications

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“…However, the absence of effective tissue culture systems for most cypoviruses means that there is little direct evidence concerning genome segment reassortment between different isolates. The identification of Cypovirus species is therefore based primarily on an analysis of the virus genome, demonstrating either a distinctive dsRNA electropherotype pattern (as originally suggested by Payne and Rivers [22]), or more recently by a comparison of the RNA sequences (e.g., by comparison of the polyhedrin gene-genome segment 10) (20). Most of the cypoviruses previously characterized contain 10 dsRNA genome segments (Seg-1 to Seg-10) named in order of decreasing size (20), although an 11th segment (Seg-11) has been reported in some cases (4,24).…”

Section: Discussionmentioning

confidence: 99%

“…Different cypovirus "types" were initially identified on the basis of differences in the migration patterns of their genome segments during polyacrylamide gel electrophoresis, i.e., electropherotypes (22). Subsequent studies showed a good correlation between electropherotype and the grouping of strains on the basis of serological properties (18) or RNA cross-hybridization (17,20).…”

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Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Shapiro

Green

Rao

et al. 2005

J Virol

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A novel cypovirus has been isolated from the mosquito Uranotaenia sapphirina (UsCPV) and shown to cause a chronic infection confined to the cytoplasm of epithelial cells of the gastric ceca and posterior stomach. The production of large numbers of virions and inclusion bodies and their arrangement into paracrystalline arrays gives the gut of infected insects a distinctive blue iridescence. The virions, which were examined by electron microscopy, are icosahedral (55 to 65 nm in diameter) with a central core that is surrounded by a single capsid layer. They are usually packaged individually within cubic inclusion bodies (polyhedra, ϳ100 nm across), although two to eight virus particles were sometimes occluded together. The virus was experimentally transmitted per os to several mosquito species. The transmission rate was enhanced by the presence of magnesium ions but was inhibited by calcium ions. Most of the infected larvae survived to adulthood, and the adults retained the infection. Electrophoretic analysis of the UsCPV genome segments (using 1% agarose gels) generated a migration pattern (electropherotype) that is different from those of the 16 Cypovirus species already recognized. UsCPV genome segment 10 (Seg-10) showed no significant nucleotide sequence similarity to the corresponding segment of the other cypoviruses that have previously been analyzed, and it has different "conserved" termini. A BLAST search of the UsCPV deduced amino acid sequence also showed little similarity to Antheraea mylitta CPV-4 (67 of 290 [23%]) or Choristoneura fumiferana CPV-16 (33 of 111 [29%]). We conclude that UsCPV should be recognized as a member of a new Cypovirus species (Cypovirus 17, strain UsCPV-17).

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Shapiro

Green

Rao

et al. 2005

J Virol

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“…Based on the variations in the electrophoretic migration patterns of the genomic dsRNA segments, 14 types of CPV have been identified (5,23,32,33). The polyhedrin has a molecular mass ranging from 27 to 31 kDa, and the smallest genome segment encodes the polyhedrin.…”

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Molecular Characterization of Bombyx mori Cytoplasmic Polyhedrosis Virus Genome Segment 4

Ikeda

Nagaoka

Winkler

et al. 2001

J Virol

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The complete nucleotide sequence of the genome segment 4 (S4) of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) was determined. The 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kDa. Western blot analysis showed that S4 encodes BmCPV protein VP3, which is one of the outer components of the BmCPV virion. Sequence analysis of the deduced amino acid sequence of BmCPV VP3 revealed possible sequence homology with proteins from rice ragged stunt virus (RRSV) S2, Nilaparvata lugens reovirus S4, and Fiji disease fijivirus S4. This may suggest that plant reoviruses originated from insect viruses and that RRSV emerged more recently than other plant reoviruses. A chimeric protein consisting of BmCPV VP3 and green fluorescent protein (GFP) was constructed and expressed with BmCPV polyhedrin using a baculovirus expression vector. The VP3-GFP chimera was incorporated into BmCPV polyhedra and released under alkaline conditions. The results indicate that specific interactions occur between BmCPV polyhedrin and VP3 which might facilitate BmCPV virion occlusion into the polyhedra.

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“…wts. of approximately 0.3 -2.5 χ IO 6 and Payne and Rivers (1976) ini tially subdivided the different isolates on the basis of major differences in the RNA pro files. Twelve types have been described (Payne andRivers 1976, Payne et al 1977).…”

Section: Cytoplasmic Polyhedrosis Virusesmentioning

confidence: 99%

The use οf νiruses for controlling pest species οf insects

Moore

Manousis

2017

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Insect viruses have been used effectively to control pest species of insects. While viruses from several families have been used, members of the Baculoviridae have proven most successful. The successful viruses are mentioned and a brief introduction into insect virus identification is followed by sufficient detail to differentiate between the major groups of viruses which have been used as field control agents. References are minimised to recent key articles on all the families of viruses found in insects.

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A Provisional Classification of Cytoplasmic Polyhedrosis Viruses Based on the Sizes of the RNA Genome Segments

Cited by 82 publications

References 20 publications

Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Morphological and Molecular Characterization of a Cypovirus ( Reoviridae ) from the Mosquito Uranotaenia sapphirina (Diptera: Culicidae)

Molecular Characterization of Bombyx mori Cytoplasmic Polyhedrosis Virus Genome Segment 4

The use οf νiruses for controlling pest species οf insects

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