A Prototype Continuous Flow Polymerase Chain Reaction LTCC Device

Moeller, Korey; Besecker, Jason; Hampikian, Greg; Moll, A.; Plumlee, Donald; Youngsman, John; Hampikian, J.M.

doi:10.4028/0-87849-428-6.523

Cited by 2 publications

(4 citation statements)

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“…The material is supplied as a thin flexible "tape", which can be worked and stacked in layers to make various types of devices. Our lab is currently working on constructing a continuous flow PCR device [13] out of low temperature ceramic tape (LTCC). It has good thermal properties, affords easy integration of electrical and mechanical components, and fabrication of the fully integrated three-dimensional devices is relatively simple and inexpensive [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Our lab is currently working on constructing a continuous flow PCR device [13] out of low temperature ceramic tape (LTCC). It has good thermal properties, affords easy integration of electrical and mechanical components, and fabrication of the fully integrated three-dimensional devices is relatively simple and inexpensive [13][14][15]. Once fired, the devices are highly resistant to chemical and thermal degradation [13,16].…”

Section: Introductionmentioning

confidence: 99%

“…It has good thermal properties, affords easy integration of electrical and mechanical components, and fabrication of the fully integrated three-dimensional devices is relatively simple and inexpensive [13][14][15]. Once fired, the devices are highly resistant to chemical and thermal degradation [13,16]. Another advantage in using LTCC is that it possesses the same thermal expansion coefficient as the silver paste used for the circuits.…”

Section: Introductionmentioning

confidence: 99%

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Dynamic passivation with BSA overcomes LTCC mediated inhibition of PCR

Besecker

Cornell

Hampikian

2013

Sensors and Actuators B: Chemical

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The increasing use of low temperature co-fired ceramic (LTCC) for the fabrication of biological microfluidic devices necessitates further research on LTCC biocompatibility. In this study we explore the inhibitory effect of DuPont's 951 LTCC on Polymerase Chain Reaction (PCR), and demonstrate a novel mechanism to increase biocompatibility between LTCC and PCR with the addition of a common passivation substance, bovine serum albumin (BSA). We show that DuPont's 951 LTCC binds negatively charged proteins including BSA and ovalbumin (OVA). This is a significant discovery as proteins (enzymes) are an essential component of most biological reactions, and a frequent addition to microfluidic devices. A proposed model for LTCC inhibition of PCR by enzyme adsorption is presented.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dynamic passivation with BSA overcomes LTCC mediated inhibition of PCR

Besecker

Cornell

Hampikian

2013

Sensors and Actuators B: Chemical

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“…4 LTCC is currently being developed for highly integrated microsystems and microfluidic devices for chemical analysis, materials synthesis, and polymerase chain reaction (PCR). [5][6][7][8] LTCC utilizes a layered assembly process and is compatible with a wide range of metallic and ceramic materials, making it a logical choice for the integration of complex, truly three-dimensional structures and multifunctional materials. Since LTCC is a true packaging technology, LTCC modules are more durable than polymeric substrates and there is no need for an additional protective envelope as when utilizing Si-based MEMS devices.…”

Section: Introductionmentioning

confidence: 99%

Magnetic hydrogel nanocomposites as remote controlled microfluidic valves

et al. 2009

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In recent years, hydrogels have attracted attention as active components in microfluidic devices. Here, we present a demonstration of remote controlled flow regulation in a microfluidic device using a hydrogel nanocomposite valve. To create the nanocomposite hydrogel, magnetic nanoparticles were dispersed in temperature-responsive N-isopropylacrylamide (NIPAAm) hydrogels. The swelling and collapse of the resultant nanocomposite can be remotely controlled by application of an alternating magnetic field (AMF). A ceramic microfluidic device with Y-junction channels was fabricated using low temperature co-fired ceramic (LTCC) technology. The nanocomposite was incorporated as a valve in one of the channels of the device. An AMF of frequency 293 kHz was then applied to the device and ON-OFF control on flow was achieved. A pressure transducer was placed at the inlet of the channel and pressure measurements were done for multiple AMF ON-OFF cycles to evaluate the reproducibility of the valve. Furthermore, the effect of the hydrogel geometry on the response time was characterized by hydrogels with different dimensions. Magnetic hydrogel nanocomposite films of different thicknesses (0.5, 1, 1.5 mm) were subjected to AMF and the kinetics of collapse and recovery were studied.

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A Prototype Continuous Flow Polymerase Chain Reaction LTCC Device

Cited by 2 publications

References 0 publications

Dynamic passivation with BSA overcomes LTCC mediated inhibition of PCR

Dynamic passivation with BSA overcomes LTCC mediated inhibition of PCR

Magnetic hydrogel nanocomposites as remote controlled microfluidic valves

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