A Protocol for Rapid and High-Frequency In Vitro Propagation of Solanum nigrum L.

Zou, Lijuan; Yang, Jingtian; Wu, Qiulan

doi:10.17576/jsm-2017-4608-03

Cited by 9 publications

(4 citation statements)

References 24 publications

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“…Roots have been was used as explants in many species, and they exhibited low regeneration compared with other explants (Zeng et al, 2019;Banjac et al, 2019;Zou et al, 2017). This experiment indicated that adventitious roots (Fig.…”

Section: Discussionmentioning

confidence: 97%

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

Cheng

Yang

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Background: As a highly valued medicinal plant, Salvia plebeia R. Brown belongs to the Lamiaceae family that has been subjected to over exploitation in its natural habitat for phytochemical and pharmacological studies. Alternative collection methods need to be developed for the large-scale propagation of Salvia plebeian.Results: Here, e cient and simple, direct organogenesis (from shoot tips and cotyledonary nodes explants) and Globular bodies (GBs) induction (from hypocotyl explants) systems were developed for the in vitro propagation of Salvia plebeia. The highest and number of regenerated shoots (7.0±0.82) per shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 0.1 mg L -1 indole-3-acetic acid (IAA) and 1.0 mg L -1 6-benzyladenine (6-BA), the proliferation of shoots and shoots rooted were carried out on the same medium treatments almost synchronously. Similarly, MS medium supplemented with 0.1 mg L -1 IAA and 1.0 mg L -1 thidiazuron (TDZ) yielded the maximum number of shoots (37.5±1.34) with 100% shoot sprouting frequency. Simultaneously, a protocol was developed for GBs induction from hypocotyl explants, and it produced 17.4 GBs per explant with 82.7% response on MS medium supplemented with TDZ (1.0 mg L -1 ) and IAA (0.1 mg L -1 ), and produced GBs that were morphologically similar to globular embryos and successfully germinated on hormone-free MS medium. The acclimatized plantlets with well-developed root systems were successfully shifted to the natural soils with a 100% survival rate.Conclusions: Taken together, this protocol can be e ciently used for mass propagation, germplasm preservation and likely also for gene transfer of Salvia plebeia.

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Section: Discussionmentioning

confidence: 97%

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

Cheng

Yang

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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“…This difference in callusing ability suggests that the tissues contain varying levels of endogenous growth regulators. Shoot bud formation was suppressed in S. torvum on MS medium with all of the auxins tested, but callusing was promoted, as seen in medicinal plants such as S. surattense (Rama Swamy et al, 2004;Rama Swamy et al, 2005) and S. nigrum (Zou et al, 2017).…”

Section: Callus Inductionmentioning

confidence: 92%

“…Many factors influence successful callus induction, including genotype, explants, culture media, PGRs, and culture conditions (Sarin, 2005). It has previously been demonstrated for many Solanaceae species for callusing from roots, leaves, hypocotyls, nodal segments, and petiole explants of S.viarum (Mahadev et al, 2014), S. nigrum (Zou et al, 2017), S. melongena (Alim et al, 2014) and S. surattense (Rama Swamy et al, 2004).…”

Section: Callus Inductionmentioning

confidence: 95%

“…Nutrient medium supplemented with PGRs viz., auxins or auxin-cytokinin combination and concentration and also the culture conditions during the incubation period play an important role in plant regeneration (Rama Swamy et al, 2004;Rama Swamy et al, 2005). The effect of these factors was reported on invitro regeneration in Solanum lycopersicum (Praveen and Rama Swamy, 2011), S. surattense (Rama Swamy et al, 2004;Rama Swamy et al, 2005), S. nigrum (Zou et al, 2017) and S. torvum (Ghan Singh et al, 2022a, 2022b.…”

Section: Plant Regeneration/organogenesismentioning

confidence: 96%

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A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

Maloth¹,

Marka

Nanna

2023

EJBIO

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In this review, we report on the different plant growth regulators (PGRs) affecting the callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from different explants and micropropagation through shoot tip and nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of this plant guard against liver and kidney damage, stop certain cancers, and regulate blood sugar levels. They benefit digestion, the management of gout and menstruation, the treatment of anaemia and diabetes, the prevention of intestinal worms, cardiovascular disorders, and strokes. The protocol developed for the induction of callus can be utilized to isolate pharmaceutically important secondary metabolites in S. torvum, and the regeneration protocols optimized in this study can be used for genetic transformation and conservation of S. torvum, not only as a medicinal plant but also a model system.

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A convenient, reliable and directly visual selection marker for identifying transgenic lines of Solanum nigrum

Peng

Wang

Wei

et al. 2022

Plant Cell Tiss Organ Cult

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A Protocol for Rapid and High-Frequency In Vitro Propagation of Solanum nigrum L.

Cited by 9 publications

References 24 publications

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

A Review on In Vitro Regeneration of Ethnomedicinal Plant Turkey Berry (Solanum torvum Swartz)

A convenient, reliable and directly visual selection marker for identifying transgenic lines of Solanum nigrum

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