A protocol for preparation and transfection of rat entorhinal cortex organotypic cultures for electrophysiological whole-cell recordings

Cilz, Nicholas I.; Porter, James E.; Lei, Saobo

doi:10.1016/j.mex.2017.10.003

MethodsX

2017

DOI: 10.1016/j.mex.2017.10.003

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A protocol for preparation and transfection of rat entorhinal cortex organotypic cultures for electrophysiological whole-cell recordings

Nicholas I. Cilz

James E. Porter

Saobo Lei

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2020

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“…The dissecting medium can be modified to include N-Methyl-D-glucamine (NMDG) in place of sodium [34]. The NMDG formulation can improve slice health in both acute [34] and organotypic preparations [35].…”

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confidence: 99%

Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method

Church

Gold

2020

Methods in Molecular Biology

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Cultured hippocampal slices from rodents, in which the architecture and functional properties of the hippocampal network are largely preserved, have proved to be a powerful substrate for studying healthy and pathological neuronal mechanisms. Here, we delineate the membrane-interface method for maintaining organotypic slices in culture for several weeks. The protocol includes procedures for dissecting hippocampus from rat brain, and collecting slices using a vibratome. This method provides the experimenter with easy access to both the brain tissue and culture medium, which facilitates genetic and pharmacological manipulations and enables experiments that incorporate imaging and electrophysiology. The method is generally applicable to rats of different ages, and to different brain regions, and can be modified for culture of slices from other species including mice.

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mentioning

confidence: 99%

Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method

Church

Gold

2020

Methods in Molecular Biology

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A protocol for preparation and transfection of rat entorhinal cortex organotypic cultures for electrophysiological whole-cell recordings

Abstract: Graphical abstract

Cited by 1 publication

References 14 publications

Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method

Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface Method

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