A protocol for in vitro maturation and fertilization of sheep oocytes

Wang, S; Liu, Y; Holyoak, G. Reed; Evans, Ronald C.; Bunch, Thomas D.

doi:10.1016/s0921-4488(97)00098-9

Cited by 18 publications

(9 citation statements)

References 23 publications

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“…), our findings are in agreement with those of Wang et al. () in which neither of these stages of nuclear maturation was observed after 27 h. This could be explained by the fact that the oocytes retrieved in this study showed more rapid nuclear maturation than those reported in other studies (Kochhar et al. ), and thus, by the time of analysis (24 h), oocytes had already reached MI and MII stages.…”

Section: Discussionsupporting

confidence: 93%

“…Germinal vesicle (GV) and germinal vesicle breakdown (GVBD) stages were not observed in this study after 24 h of IVM. While some authors report these stages after 24 h (Shi et al 2009), our findings are in agreement with those of Wang et al (1998) in which neither of these stages of nuclear maturation was observed after 27 h. This could be explained by the fact that the oocytes retrieved in this study showed more rapid nuclear maturation than those reported in other studies (Kochhar et al 2002), and thus, by the time of analysis (24 h), oocytes had already reached MI and MII stages. We believe, however, that if these oocytes had been analysed before 24 h, the GV and GVBD stages would have been observed.…”

Section: Discussionsupporting

confidence: 90%

“…Previous studies report nuclear maturation MII rates, from viable oocytes, of 90.1% (Baldassarre et al 1994a) and 75.1% (Berlinguer et al 2006) for oocytes retrieved by laparoscopy and 94.2% (Baldassarre et al 1994a), 84% (Ledda et al 1997), 70.2% (Wang et al 1998), 83% (Kochhar et al 2002), 73.1% (Shirazi et al 2008) and 81.3% (Shi et al 2009) from ovaries collected at slaughterhouses. The rates of MII (32.72%) observed in this study were lower than those previously reported but similar to those registered by Freitas et al (2007) for ovine (33.3%) and caprine oocytes (48.9%) although the authors do not specify the technique used for retrieval.…”

Section: Discussionmentioning

confidence: 99%

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In vitro Maturation of Oocytes from Santa Ines Ewes Subjected to Consecutive Sessions of Follicular Aspiration by Laparoscopy

Padilha

Teixeira

Pires-Buttler

et al. 2013

Reprod Domestic Animals

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The success of embryo production in vitro depends upon the use of an efficient oocyte retrieval technique, and the best results have been obtained by laparoscopic aspiration. The aim of this study was to evaluate the effect of consecutive sessions of follicular aspiration on the quantity, quality and in vitro maturation competence of oocytes obtained from ewes subjected to hormonal stimulation. Six Santa Ines ewes underwent nine sessions of follicular aspiration by laparoscopy with a 7-day interval between sessions, totalling 56 aspirations. After 24 h of culture, oocytes were stained and classified according to the stage of nuclear and cytoplasmic maturation. Oocyte retrieval rate was 61.4 ± 2%, resulting in a total of 249 oocytes. No significant variation was observed between sessions (p > 0.05). The average number of oocytes retrieved from each ewe was 6.4 ± 2 per session and 42 ± 4 in total. No significant difference was observed between the frequencies of the different stages of nuclear maturation: 32.72% mature, 40.74% immature and 26.54% degenerated/indeterminate oocytes; however, a significant difference was observed between the frequencies of the different stages of cytoplasmic maturation: 10.7% mature, 73.25% immature and 16.05% degenerated/indeterminate oocytes. No significant difference was observed in nuclear or cytoplasmic maturation between the weeks of procedure. We conclude that after nine consecutive sessions of follicular aspiration, the quantity and quality of retrieved oocytes remained unchanged as well as the levels of nuclear and cytoplasmic maturation obtained, demonstrating the viability of this technique for repetitive follicular aspirations on the same donor.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro Maturation of Oocytes from Santa Ines Ewes Subjected to Consecutive Sessions of Follicular Aspiration by Laparoscopy

Padilha

Teixeira

Pires-Buttler

et al. 2013

Reprod Domestic Animals

View full text Add to dashboard Cite

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“…From the present findings it could be suggested that with increasing doses (0.1 µM to 0.2 µM) of calcium ionophore, embryo development potential decreases (Table 2). Earlier reports also support the present findings (4,26).…”

Section: Discussionsupporting

confidence: 93%

“…The results suggest that calcium ionophore (0.1 µM) is effective for sperm pretreatment to induce capacitation during in vitro fertilization and subsequent in vitro development. Wang et al (26) found higher cleavage, morula, and blastocyst production rates in sperm treatment with 0.1 µM of calcium ionophore+caffeine as compared to another sperm treatment (with 5% ewe estrous serum).…”

Section: Discussionmentioning

confidence: 99%

Effect of capacitating agents on sperm pretreatment during in vitrofertilization for blastocyst production in caprines

Goel¹,

Goel²,

Bhatiya³

et al. 2016

Turk J Vet Anim Sci

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The present study aimed at comparing the relative efficacy of different capacitating agents used for sperm pretreatment during in vitro fertilization (IVF) and subsequent embryo development of caprine oocytes. In experiment 1, the effect of different concentrations of heparin (0, 20, and 50 µg/mL) on sperm pretreatment for different periods of time (30 and 60 min) was studied. In experiment 2, the dose-dependent effect of calcium ionophore (0, 0.1, 0.2, and 0.5 µM) on sperm pretreatment was assessed. Experiment 3 validated and compared the effects of both capacitating agents on sperm pretreatment for capacitation based on the results of the first two experiments. The treated sperms from each group were used for in vitro fertilization and subsequent embryo development. The results indicated that in experiment 1, capacitation with heparin at 20 µg/mL for 60 min had significantly higher (P < 0.05) cleavage and blastocyst production. In experiment 2, capacitation with calcium ionophore at 0.1 µM had significantly more morulae (P < 0.05) and numerically more blastocyst production. In experiment 3, capacitation with heparin at 20 µg/mL had a significantly higher (P < 0.05) blastocyst production rate as compared to calcium ionophore treatment. Based on this study, heparin can be used to enhance capacitation of freshly collected sperms during in vitro fertilization and subsequent embryo development.

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Livestock Biodiversity

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A protocol for in vitro maturation and fertilization of sheep oocytes

Cited by 18 publications

References 23 publications

In vitro Maturation of Oocytes from Santa Ines Ewes Subjected to Consecutive Sessions of Follicular Aspiration by Laparoscopy

In vitro Maturation of Oocytes from Santa Ines Ewes Subjected to Consecutive Sessions of Follicular Aspiration by Laparoscopy

Effect of capacitating agents on sperm pretreatment during in vitrofertilization for blastocyst production in caprines

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