A protocol for efficient biolistic transformation of mothbeanVigna aconitifolia L. Jacq. Marechal

Kamble, Suchita; Misra, Hari S.; Mahajan, Surabhi; Eapen, Susan

doi:10.1007/bf02772595

Cited by 16 publications

(8 citation statements)

References 12 publications

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“…The construction of pHS101 and pHS102 plasmids was exactly similar, except that in pHS101, a waxy locus leader peptide was fused with the transgene, which targets the synthesized protein to chloroplasts. In earlier studies on particle gun bombardment mediated gene transfer in V. aconitifolia (Kamble et al 2003) the influence of plasmid constructs on the transformation frequency was reported. The present results on chickpea that pHS102 produced the highest stable transformation frequency is in agreement with the report on V. aconitifolia transformation (Kamble et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…To study the influence of different Agrobacterium strains, disarmed Agrobacterium tumefaciens strain LBA4404 harboring three plasmids pHS101, pHS102 (Kamble et al 2003) and pBI121 (Clonetech, USA) were used for transformation experiments. All the three plasmids used were binary vectors containing selectable marker gene nptII and reporter gene uidA driven by CaMV35S promoter.…”

Section: Agrobacterium Strain and Plasmidsmentioning

confidence: 99%

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Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Indurker

Misra

Eapen

2010

Physiol Mol Biol Plants

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Agrobacterium-mediated transformation in chickpea was developed using strain LBA4404 carrying nptII, uidA and cryIAc genes and transformants selected on Murashige and Skoog's basal medium supplemented with benzyladenine, kinetin and kanamycin. Integration of transgenes was demonstrated using polymerase chain reaction and Southern blot hybridization of T 0 plants. The expression of CryIAc delta endotoxin and GUS enzyme was shown by enzyme linked immunosorbent assay and histochemical assay respectively. The transgenic plants (T 0 ) showed more tolerance to infection by Helicoverpa armigera compared to control plants. Various factors such as explant source, cultivar type, different preculture treatment period of explants, co-cultivation period, acetosyringone supplementation, Agrobacterium harboring different plasmids, vacuum infiltration and sonication treatment were tested to study the influence on transformation frequency. The results indicated that use of epicotyl as explant, cultivar ICCC37, Agrobacterium harboring plasmid pHS102 as vector, preculture of explant for 48 h, cocultivation period of 2 days at 25°C and vacuum infiltration for 15 min produced the best transformation results. Sonication treatment of explants with Agrobacteria for 80 s was found to increase the frequency of transformation.

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Section: Discussionmentioning

confidence: 99%

Section: Agrobacterium Strain and Plasmidsmentioning

confidence: 99%

Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Indurker

Misra

Eapen

2010

Physiol Mol Biol Plants

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“…(Kathju et al, 2003;Khatri et al, 2004 andRathore, 2001). Genetic transformation of moth bean has been achieved using particle bombardment or Agrobacterium -mediated transfer (Kamble et al, 2003).…”

Section: Genetic Resourcesmentioning

confidence: 99%

Diversity of Potential Orphan Plants in Health Management and Climate Change Mitigation from Bahraich (Uttar Pradesh), India

Mall¹

2017

Int. J. Curr. Res. Biosci. Plant Biol.

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Crop species which are adapted to hot and dry climates will become increasingly important as the world worms. The Krikhouse Trust* supporting research and education in the biological Sciences is devoted for agricultural crop improvement for the relief of poverty, with a focus on legumes. The trust has supported research on legumes because of their importance in providing high quality protein in the diets of resource-poor farmers. Among these crops are many stress tolerant legume species found in India and Africa, which are relatively minor and neglected crops. A new programme called -Stress Tolerant Orphan Legumes‖ (STOL) for KT aims to support systematic studies of their potential to address the loss of agricultural productivity in areas of the globe that are suffering the greatest climate stresses. There are clear signs that climate change is already having severe effects on the agriculture where several crops are failing as result of the changed climate. In addition to direct effects of heat and drought on crops, the country is suffering soil loss due to increase in desert area per year. Over grazing, intensive cultivation, removal of tree cover, poor water management have all been as a reasons for this loss which cannot be compensated by any means. One more versatile fact remains that large tracts of land are being taken out of production for different types of constructions by the governments for the people as well as by the people for their settlements in urban areas. There are three possible ways to mitigate this looming disaster. First one is the adoption of stress tolerant legume species which may help farmers to fight the ravages of climate stress: production of grain to feed the family and also provide income; fodder and forage to livestock; ground cover as remedy for soil degradation. For this reason there should be a diversity of species where some crops may prove to be multipurpose, providing grain and fodder, forage and other crops may be specialized for other function such as soil remediation. In this study, only twelve orphan legumes are taken, viz., Cajanus cajan, Lablab purpureus,

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“…However, in barley, mothbean and of the GUS spots spread out of the meristem region. olive, 900 psi helium pressure was found to be optimum Therefore, in the subsequent experiments, a 6 cm target (Odake, 2004;Kamble et al, 2003;Perez-Barranco et al, distance and 1100 psi helium pressure were used. 2009).…”

Section: Effect Of Helium Pressurementioning

confidence: 99%

“…distance causes reduction in the average number o f Effect of target distance: Figure 3 shows that the increase be significantly better than other distances in mothbean, in the target distance caused reduction in the average cowpea, wheat, castor and olive (Kamble et al, 2003; number of GUS spots/explant and percentage of explants Ikea et al, 2003;Odake, 2004;Sailaja et al, 2008;expressing 125 was used as the target distance (Yin et al, 2009). This were significantly reduced.…”

Section: Effect Of Helium Pressurementioning

confidence: 99%

Biolistic Transformation of Citrullus vulgaris Schrad (Watermelon)

Suratman

Huyop²,

Wagiran³

et al. 2010

Biotechnology

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Abstract:In this study, DNA-coated gold particles were used to transform cotyledon sections including axillary meristem of Citrullus vulgaris using the biolistic method for optimizing the transformation parameters and eventually regeneration of putative transgenic watermelon plants. Initially, five biolistic parameters namely helium pressure, macrocarrier to target tissue distance, explant ages, DNA concentrations and osmotic treatment were optimized using plasmid pRQ6 (carrying both gusA and hpt genes). The optimized biolistic parameters for cotyledon sections of Citrullus vulgaris explants were determined as follow: 1100 psi helium pressure, 6 cm target distance, 4-day-old explants, concentration of DNA at 1.2 µg/bombardment and osmotic treatment for 24 h on medium supplemented with 0.6 M mannitol prior to bombardment. Using the optimized parameters, transformation of watermelon cotyledons was carried out using pAHG11, pCAMBIA 1301, co-transformation (pAHG11+pCAMBIA 1301) and pCambar plasmids. Bombarded tissue's were selected on either 5 mg LG 1 hygromycin or 2 mg LG PPT. Finally, the transformants produced were subjected to GUS histochemical assay. 1Integration of the transgenes into transgenic watermelon genome was confirmed by PCR analysis.

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A protocol for efficient biolistic transformation of mothbeanVigna aconitifolia L. Jacq. Marechal

Cited by 16 publications

References 12 publications

Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Diversity of Potential Orphan Plants in Health Management and Climate Change Mitigation from Bahraich (Uttar Pradesh), India

Biolistic Transformation of Citrullus vulgaris Schrad (Watermelon)

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