A Proteomics Study of Brassinosteroid Response in Arabidopsis

Deng, Zhiping; Zhang, Xin; Tang, Wenqiang; Oses-Prieto, Juan A.; Suzuki, Nagi; Gendron, Joshua M.; Chen, Huanjing; Guan, Shenheng; Chalkley, Robert J.; Peterman, T. Kaye; Burlingame, Alma L.; Wang, Wenfei

doi:10.1074/mcp.m700123-mcp200

Cited by 184 publications

(154 citation statements)

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“…Several lines of evidence indicate that using IP linked to mass spectrometry analysis is a powerful tool to identify true interactors of the BRL3 receptor complex. First, we purified the BRL3 receptor complex in Arabidopsis and identified a number of interactors of BRI1, which have been previously characterized as BR signaling components (Li et al, 2002;Deng et al, 2007). In addition, our analysis revealed a number of potential interactors that coimmunoprecipitated with the BRL3 receptor protein.…”

Section: Discussionmentioning

confidence: 99%

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

Fàbregas

Boeren

et al. 2013

The Plant Cell

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Brassinosteroid (BR) hormones are primarily perceived at the cell surface by the leucine-rich repeat receptor-like kinase BRASSINOSTEROID INSENSITIVE1 (BRI1). In Arabidopsis thaliana, BRI1 has two close homologs, BRI1-LIKE1 (BRL1) and BRL3, respectively, which are expressed in the vascular tissues and regulate shoot vascular development. Here, we identify novel components of the BRL3 receptor complex in planta by immunoprecipitation and mass spectrometry analysis. Whereas BRI1 ASSOCIATED KINASE1 (BAK1) and several other known BRI1 interactors coimmunoprecipitated with BRL3, no evidence was found of a direct interaction between BRI1 and BRL3. In addition, we confirmed that BAK1 interacts with the BRL1 receptor by coimmunoprecipitation and fluorescence microscopy analysis. Importantly, genetic analysis of brl1 brl3 bak1-3 triple mutants revealed that BAK1, BRL1, and BRL3 signaling modulate root growth and development by contributing to the cellular activities of provascular and quiescent center cells. This provides functional relevance to the observed protein-protein interactions of the BRL3 signalosome. Overall, our study demonstrates that cell-specific BR receptor complexes can be assembled to perform different cellular activities during plant root growth, while highlighting that immunoprecipitation of leucine-rich repeat receptor kinases in plants is a powerful approach for unveiling signaling mechanisms with cellular resolution in plant development.

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Section: Discussionmentioning

confidence: 99%

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

Fàbregas

Boeren

et al. 2013

The Plant Cell

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“…"sup" identifies m␤cd responsiveness based on release of these proteins to the supernatant after pelleting m␤cd-treated plasma membranes. Letters indicate proteins previously published as being GPI-anchored (gpi) (35,36,50,51) …”

Section: Core List Of Proteins Identified As M␤cd-responsive In At Lementioning

confidence: 99%

Definition of Arabidopsis Sterol-rich Membrane Microdomains by Differential Treatment with Methyl-β-cyclodextrin and Quantitative Proteomics

Kierszniowska

Seiwert

Schulze

2009

Molecular & Cellular Proteomics

119

113

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Plasma membranes are dynamic compartments with key functions in solute transport, cell shape, and communication between cells and the environment. In mammalian cells and yeast, the plasma membrane has been shown to be compartmented into so-called lipid rafts, which are defined by their resistance to treatment with non-ionic detergents. In plants, the existence of lipid rafts has been postulated, but the precise composition of this membrane compartment is still under debate. Here we were able to experimentally clearly distinguish (i) true sterol-dependent "raft proteins" and (ii) sterol-independent "non-raft" proteins and co-purifying "contaminants" in plant detergent-resistant membranes. We used quantitative proteomics techniques involving 15 N metabolic labeling and specific disruption of sterol-rich membrane domains by methyl-␤-cyclodextrin. Among the sterol-dependent proteins we found an over-representation of glycosylphosphatidylinositol-anchored proteins. A large fraction of these proteins has functions in cell wall anchoring. We were able to distinguish constant and variable components of plant sterol-rich membrane microdomains based on their responsiveness to the drug methyl-␤-cyclodextrin. Predominantly proteins with signaling functions, such as receptor kinases, G-proteins, and calcium signaling proteins, were identified as variable members in plant lipid rafts, whereas cell wall-related proteins and specific proteins with unknown functions make up a core set of sterol-dependent plant plasma membrane proteins. This allows the plant to maintain a balance between static anchoring of cell shape forming elements and variable adjustment to changing external conditions. Molecular & Cellular Proteomics 8:612-623, 2009.

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“…Glutathione and its metabolic enzymes interact with plant growth regulators, which are regarded as vital in the scheme of plant establishment both under normal and stressful conditions. GST expression in plants is induced by phytohormones such as SA, ethylene, cytokinin, auxin, ABA (Marrs 1996), methyl jasmonate (Moons 2003), and brassinosteroids (Deng et al 2007). Chen et al (2012) demonstrated that GST overexpressed in Arabidopsis thaliana plants had a signaling role and it regulated plant development by maintaining GSH pools.…”

Section: Droughtmentioning

confidence: 99%

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Hasanuzzaman

Nahar

Anee

et al. 2017

Physiol Mol Biol Plants

596

341

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Glutathione (GSH; c-glutamyl-cysteinyl-glycine) is a small intracellular thiol molecule which is considered as a strong non-enzymatic antioxidant. Glutathione regulates multiple metabolic functions; for example, it protects membranes by maintaining the reduced state of both a-tocopherol and zeaxanthin, it prevents the oxidative denaturation of proteins under stress conditions by protecting their thiol groups, and it serves as a substrate for both glutathione peroxidase and glutathione S-transferase. By acting as a precursor of phytochelatins, GSH helps in the chelating of toxic metals/metalloids which are then transported and sequestered in the vacuole. The glyoxalase pathway (consisting of glyoxalase I and glyoxalase II enzymes) for detoxification of methylglyoxal, a cytotoxic molecule, also requires GSH in the first reaction step. For these reasons, much attention has recently been directed to elucidation of the role of this molecule in conferring tolerance to abiotic stress. Recently, this molecule has drawn much attention because of its interaction with other signaling molecules and phytohormones. In this review, we have discussed the recent progress in GSH biosynthesis, metabolism and its role in abiotic stress tolerance.

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A Proteomics Study of Brassinosteroid Response in Arabidopsis

Cited by 184 publications

References 100 publications

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

Definition of Arabidopsis Sterol-rich Membrane Microdomains by Differential Treatment with Methyl-β-cyclodextrin and Quantitative Proteomics

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

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