A proteomics of gills approach to understanding salinity adaptation of Scylla paramamosain

Wang, Huan; Wei, Hongling; Tang, Lei; Lu, Junkai

doi:10.1016/j.gene.2018.07.059

Cited by 32 publications

(29 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The other three groups were defined as the CK groups, in which the salinity of seawater was maintained at 23‰. All other parameters in this group were the same as in the LS group [32, 33].…”

Section: Methodsmentioning

confidence: 99%

“…The analysis of transcriptome implied that ion transport and amino acid metabolism were key factors in regulating the salinity adaptation of S. paramamosain , but ion transport might be more important than amino acid metabolism in the regulatory process [32]. However, the results of proteome implied that amino acid metabolism played a more important role in the process of adaption for Scylla paramamosain [33]. So, in this study, a correlation analysis of RNA sequencing transcriptomics and iTRAQ-tandem MS proteomics was used to further explore the key genes of correlations for DEPs_DEGs Same Trend and their involved molecular mechanisms responding to salinity adaptation in Scylla paramamosain .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation inScylla paramamosain

Wang

Wei

Tang

et al. 2019

International Journal of Genomics

Self Cite

View full text Add to dashboard Cite

Scylla paramamosain is a commercially important species distributed along the coast of southern China and other Indo-Pacific countries. Sudden salinity drop exceeding the adjustment capability of S. paramamosain can result in damage or even mortality. In our previous study, we had analyzed the mechanism of adapting sudden drop in salinity from the level of transcriptomics and proteomics, respectively. This study performed a correlation analysis of RNA sequencing transcriptomics and iTRAQ proteomics in order to investigate the adaptation mechanisms to sudden salinity drop from 23‰ to 3‰. There were 3954 correlations and a total of 15 correlations for differentially expressed proteins (DEPs) and differentially expressed genes (DEGs) from proteomics and transcriptomics. The correlation between DEPs and DEGs was 0, and the Spearman correlation coefficient of the same trend correlation for DEPs and DEGs was the highest, reaching 0.9080. KEGG pathway enrichment correlation revealed that protein digestion and absorption (Ko04974), proximal tubule bicarbonate (Ko04964), and bile secretion (Ko04976) played important roles in Na+/H+ and Na+/K+ exchange. In addition, important genes related to osmoregulation, such as ion transport and carbonic anhydrase, were also detected in the correlation analysis for same trend DEPs_DEGs. In conclusion, the proteome and transcriptome correlation results from this study indicate that ion transport plays a critical role in the adaptation of S. paramamosain to sudden reduction in salinity.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation inScylla paramamosain

Wang

Wei

Tang

et al. 2019

International Journal of Genomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The peptides fractionation, high performance liquid chromatography (HPLC), and mass spectrometer detection were performed using the previously described procedures (Wang et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells

Zheng

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

Intestinal microbial interactions with the host epithelium have important roles in host health. Our previous data have suggested that Lactobacillus gasseri LA39 is the predominant intestinal Lactobacillus in weaned piglets. However, the regulatory role of L. gasseri LA39 in the intestinal epithelial protein expression in piglets remains unclear. In the present study, we conducted comparative proteomics approach to investigate the intestinal epithelial protein profile alteration caused by L. gasseri LA39 in piglets. The expressions of 15 proteins significantly increased, whereas the expressions of 13 proteins significantly decreased in the IPEC-J2 cells upon L. gasseri LA39 treatment. Bioinformatics analyses, including COG function annotation, GO annotation, and KEGG pathway analysis for the differentially expressed proteins revealed that the oxidative phosphorylation (OXPHOS) pathway in IPEC-J2 cells was significantly activated by L. gasseri LA39 treatment. Further data indicated that two differentially expressed proteins UQCRC2 and TCIRG1, associated with the OXPHOS pathway, and cellular ATP levels in IPEC-J2 cells were significantly up-regulated by L. gasseri LA39 treatment. Importantly, the in vivo data indicated that oral gavage of L. gasseri LA39 significantly increased the expression of UQCRC2 and TCIRG1 and the cellular ATP levels in the intestinal epithelial cells of weaned piglets. Our results, both in vitro and in vivo, reveal that L. gasseri LA39 activates the OXPHOS pathway and increases the energy production in porcine intestinal epithelial cells. These findings suggest that L. gasseri LA39 may be a potential probiotics candidate for intestinal energy production promotion and confers health-promoting functions in mammals.

show abstract

“…The IPEC-J2 cells treated with L. frumenti (the ratio of bacterial strain number to cell number were 100:1) or PBS for 4 h were used for isobaric tags for relative and absolute quantitation (iTRAQ) analysis following the manufacturer's procedures, which have been described in detail in previous studies (17)(18)(19). Briefly, proteins were extracted using lysis buffer, magnetic beads, and TissueLyser, following with DTT and iodoacetamide.…”

Section: Measurement Of Cellular Total Ros Levels and Total Antioxidamentioning

confidence: 99%

Lactobacillus frumentiimproves antioxidant capacityvianitric oxide synthase 1 in intestinal epithelial cells

Nie

Hou

et al. 2019

FASEB j.

View full text Add to dashboard Cite

Oxidative damages have adverse effects on mammals. Growing studies have focused on exploring new antioxidants. Here, we report that Lactobacillus frumenti increases the total antioxidation capacity activities and decreases the total reactive oxygen species levels in porcine intestinal epithelial cells. Comparative proteomics revealed that expressions of peroxiredoxin 2, isocitrate dehydrogenase 1, NAD(P)H dehydrogenase quinone 1, antioxidant protein 1, and metallothionein‐2A, which are associated with antioxidant defense system, were significantly increased with L. frumenti treatment. In germ‐free mice, L. frumenti treatment also remarkably improves the intestinal antioxidant capacity. We further illustrated that nitric oxide production—mediated by nitric oxide synthase 1 activation is essential for L. frumenti—induced improvements in intestinal epithelial antioxidant capacity and barrier function. This study suggested that L. frumenti may be a potential probiotic used to prevent oxidative stress—induced aging and diseases in mammals.—Nie, Y., Hu, J., Hou, Q., Zheng, W., Zhang, X., Yang, T., Ma, L., Yan, X. Lactobacillus frumenti improves antioxidant capacity via nitric oxide synthase 1 in intestinal epithelial cells. FASEB J. 33, 10705–10716 (2019). http://www.fasebj.org

show abstract

A proteomics of gills approach to understanding salinity adaptation of Scylla paramamosain

Cited by 32 publications

References 46 publications

Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation inScylla paramamosain

Gene Identification and Characterization of Correlations for DEPs_DEGs Same Trend Responding to Salinity Adaptation inScylla paramamosain

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells

Lactobacillus frumentiimproves antioxidant capacityvianitric oxide synthase 1 in intestinal epithelial cells

Contact Info

Product

Resources

About