A proteomic road to acquire an accurate serological diagnosis for human tegumentary leishmaniasis

Lima, B.S.S.; Pires, Simone da Fonseca; Fialho, L.C.; Oliveira, Edward; Machado-de-Ávila, Ricardo Andrez; Chávez-Olórtegui, Carlos; Chapeaurouge, Alex; Perales, Jonás; Andrade, Hélida Monteiro de

doi:10.1016/j.jprot.2016.05.017

Cited by 17 publications

(14 citation statements)

References 34 publications

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“…The accurate diagnosis of American tegumentary leishmaniasis (ATL) is an essential task due to the disease's associated morbidity (1,2) but is hindered by the lack of a gold-standard laboratory test (3)(4)(5). Currently available parasitological tests based on stained smears and culture offer low sensitivity, and other options, such as the Montenegro (leishmanin) skin test (MST), have high sensitivity but low specificity (3,6,7).…”

mentioning

confidence: 99%

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

et al. 2017

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The precise diagnosis of American tegumentary leishmaniasis (ATL) is an essential task due to the disease's associated morbidity. A noninvasive, extremely sensitive, and highly specific exam is critical, particularly for mucosal leishmaniasis (ML), in which a low parasite quantity is expected. We aimed to compare the diagnostic accuracy of swab and biopsy sample analysis using SYBR Green-and TaqManbased real-time PCR (qPCR) assays with that of a composite reference standard consisting of the Montenegro skin test, serology, histopathology, smears, culture, and conventional PCR. In total, 55 patients with ATL (ML, 18 patients; cutaneous leishmaniasis [CL], 37 patients) and 36 patients without ATL were studied. qPCR analysis of swabs was more accurate when using SYBR Green (87.88%; 95% confidence interval [CI], 77.86 to 93.73 patients) than when using TaqMan (78.79%; 95% CI, 67.49 to 86.92%) (P ϭ 0.031). SYBR Green (84.72%; 95% CI, 74.68 to 91.25%) was also more accurate than TaqMan (73.61%; 95% CI, 62.42 to 82.41%) for biopsy samples (P ϭ 0.008). All qPCR methods were 100% specific. Swabs and biopsy specimens had similar sensitivity when using the same chemistry (P ϭ 0.125 for SYBR Green and P ϭ 0.625 for TaqMan). Moreover, qPCR achieved better performance than most existing techniques used for the diagnosis of ATL and also detected the Leishmania parasite in a greater proportion of patients than the associated histopathology, smear, culture, and conventional PCR techniques did. Swabs therefore represent a useful diagnostic tool because they not only are noninvasive but also can achieve an accuracy similar to that of biopsy samples. The high accuracy of SYBR Green-based qPCR may also reduce the requirement for associated parasitological tests for ATL diagnosis.

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confidence: 99%

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

et al. 2017

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“…Immunoproteomics has been useful for the discovery of proteins specifically recognized by sera of patients with leishmaniasis . Indeed, five proteins from L. braziliensis were selected based on this technique and were synthesized for diagnostic tests by ELISA .…”

Section: Potential Applications Of Proteomics In Leishmaniasismentioning

confidence: 99%

“…These antigens, mainly TXNPx, showed high sensitivity and specificity for leishmaniasis when tested against patients with leishmaniasis, Chagas' disease and healthy subjects . Abundant and immunogenic antigens specific for L. braziliensis , L. amazonensis , or L. infantum chagasi were selected by immunoproteomics for selective identification of leishmaniasis . Apart from serum, urine may also be used as a source for antibodies for diagnostic tests.…”

Section: Potential Applications Of Proteomics In Leishmaniasismentioning

confidence: 99%

Proteomics and Leishmaniasis: Potential Clinical Applications

Capelli-Peixoto

Mule

Tano

et al. 2019

Proteomics Clinical Apps

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Leishmaniases are diseases caused by protozoan parasites of the genus Leishmania. They are endemic in 98 countries, affect around 12 million people worldwide and may present several distinct clinical forms. Unfortunately, there are only a few drugs available for treatment of leishmaniasis, which are toxic and not always effective. Different parasite species and different clinical forms require optimization of the treatment or more specific therapies, which are not available. The emergence of resistance is also a matter of concern. Besides, diagnosis can sometimes be complicated due to atypical manifestations and associations with other pathologies. In this review, proteomic data are presented and discussed in terms of their application in important issues in leishmaniasis such as parasite resistance to chemotherapy, diagnosis of active disease in patients and dogs, markers for different clinical forms, identification of virulence factors, and their potential use in vaccination. It is shown that proteomics has contributed to the discovery of potential biomarkers for prognosis, diagnosis, therapeutics, monitoring of disease progression, treatment follow‐up and identification of vaccine candidates for specific diseases. However, the authors believe its capabilities have not yet been fully explored for routine clinical analysis for several reasons, which will be presented in this review.

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“…Since obtaining pure amastigotes in vitro is challenging, most of the previous studies preferred to select promastigotes for proteomic studies in Leishmania parasites [16][17][18]. Due to the lack of immunoproteomic studies on the Leishmania amastigote form, this study was conducted for introducing new immunoreactive proteins in amastigotes of L. infantum, using two-dimensional gel electrophoresis (2-DE) followed by Western blotting with CVL-infected dogs' sera.…”

Section: Introductionmentioning

confidence: 99%

An immunoproteomic approach to identifying immunoreactive proteins inLeishmania infantumamastigotes using sera of dogs infected with canine visceral leishmaniasis

Rashidi

Mojtahedi

Shahriari

et al. 2019

Pathogens and Global Health

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Visceral leishmaniasis (VL), the most severe form of leishmaniasis, is caused by Leishmania donovani and Leishmania infantum. The infected dogs with canine visceral leishmaniasis (CVL) are important reservoirs for VL in humans, so the diagnosis, treatment and vaccination of the infected dogs will ultimately decrease the rate of human VL. Proteomics and immunoproteomics techniques have facilitated the introduction of novel drug, vaccine and diagnostic targets. Our immunoproteomic study was conducted to identify new immunoreactive proteins in amastigote form of L. infantum. The strain of L. infantum (MCAN/IR/07/Moheb-gh) was obtained from CVLinfected dogs. J774 macrophage cells were infected with the L. infantum promastigotes. The infected macrophages were ruptured, and pure amastigotes were extracted from the macrophages. After protein extraction, two-dimensional gel electrophoresis was employed for protein separation followed by Western blotting. Western blotting was performed, using symptomatic and asymptomatic sera of the infected dogs with CVL. Thirteen repeatable immunoreactive spots were identified by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Some, including prohibitin, ornithine aminotransferase, annexin A4, and apolipoprotein A-I, have been critically involved in metabolic pathways, survival, and pathogenicity of Leishmania parasites. Further investigations are required to confirm our identified immunoreactive proteins as a biomarker for CVL.

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A proteomic road to acquire an accurate serological diagnosis for human tegumentary leishmaniasis

Cited by 17 publications

References 34 publications

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic

Proteomics and Leishmaniasis: Potential Clinical Applications

An immunoproteomic approach to identifying immunoreactive proteins inLeishmania infantumamastigotes using sera of dogs infected with canine visceral leishmaniasis

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