A proteomic approach to the investigation of early events involved in vascular smooth muscle cell activation

Boccardi, Claudia; Cecchettini, Antonella; Caselli, Anna; Camici, Giovanni G.; Evangelista, Monica; Mercatanti, Alberto; Rainaldi, Giuseppe; Citti, Lorenzo

doi:10.1007/s00441-006-0357-3

Cited by 19 publications

(12 citation statements)

References 30 publications

(29 reference statements)

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“…Hence, contrary to our expectations, our data indicated that the proteomic differences that we detected were a response to medium shift per se rather than a proteome profile for the cells grown in a specific medium. The presence of growth factors has been shown to result in protein expression changes in SMCs in several previous studies (38)(39)(40). However, in our study, although we observed changes, the changes were not in response to the presence of growth factors.…”

Section: Discussioncontrasting

confidence: 90%

Proteomic evidence for the plasticity of cultured vascular smooth muscle cells

Baykal¹,

Baykal²,

Serhatlı³

et al. 2013

Turk J Biol

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Morphological, functional, and gene expression studies have established the phenotypic plasticity of smooth muscle cells (SMCs). These cells have been shown to respond to environmental stimulants such as extracellular matrix and growth factors. Cellular changes can vary between extremes defined as the contractile and synthetic states. Various growth factors have been shown to have profound effects on the phenotype of these cells. In this study, we intended to investigate the effects of growth factor-rich medium on the protein expression of vascular SMCs in culture. Interestingly, transiently changing the type of medium did not result in any apparent morphological differences, yet we hypothesized that some cellular factors might still be altered. In order to understand what kind of intracellular molecular changes should be expected to occur during the medium change, we analyzed global protein expression changes using nano-LC-MS/MS in smooth muscle cell cultures that were isolated and grown in one medium formulation and temporarily switched to the other. Our data indicate that proteins playing a role in energy metabolism (glycolysis), translation, and folding of proteins are affected, along with regulatory molecules and cytoskeletal proteins. The individual proteins and their significance are discussed within the scope of this paper.

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Section: Discussioncontrasting

confidence: 90%

Proteomic evidence for the plasticity of cultured vascular smooth muscle cells

Baykal¹,

Baykal²,

Serhatlı³

et al. 2013

Turk J Biol

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“…Johansson et al 1997;Kacem et al 1996). Even if this is a considerable simplification (Boccardi et al 2007), changes in expression of these cytoskeletal proteins should reflect at least the basic changes of the mechanical properties of the vessel wall due to surgery sufficiently.…”

Section: Elastin Length Density In the Aortic Wall Decreased In Timementioning

confidence: 98%

“…VSMC are able to modulate their phenotype in response to injury and mechanical stimuli (Miyazaki et al 2002;Owens et al 2004;Yoshigi et al 2000), changing the mechanical properties of the vessel wall. In general, VSMC phenotype switches from contractile to synthetic after injury (Boccardi et al 2007;Miyazaki et al 2002;Owens et al 2004;Shanahan and Weissberg 1999).…”

Section: Elastin Length Density In the Aortic Wall Decreased In Timementioning

confidence: 99%

Tissue reaction to three different types of tissue glues in an experimental aorta dissection model: a quantitative approach

Witter

Tonar

Matějka

et al. 2009

Histochem Cell Biol

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Tissue glues are used during surgical treatment of acute aorta dissection although some glues release toxic products and thus alter the histological structure of the vessel wall. The aim of our study was to use a porcine experimental model of infrarenal aorta dissection to compare histological changes of the vessel wall 1, 6 and 12 months after application of BioGlue, Gelatin-resorcin-formaldehyde (GRF) glue and Tissucol. For quantification, stereological methods were used. All types of glue caused stenosis, GRF most and Tissucol least severely. With increasing postoperative survival time, stenosis was again reduced. Elastine length density decreased with increasing survival time in Control as well as in all Experimental groups. The immunohistochemical phenotype of vascular smooth muscle cells was similar in Tissucol and Control samples. In GRF samples, actin, desmin and vimentin expression changed most severely. Similarly, number and distribution of vasa vasorum in the aortic wall was altered most severely in GRF samples. They tended to return to normal with increasing postoperative survival time, but at a slow rate in the GRF samples. It can be concluded that GRF causes the most severe histopathological changes within the treated aorta, which could be a reason for late failures of dissection surgery. However, glue handling and adhesive properties have to be taken into account, too, when certain glue is chosen for surgical intervention. Increased inflammation and vascularisation might even stabilise the aortic wall. Long-term experimental studies would be helpful to assess healing processes after initial disorganisation of the aortic wall structure.

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“…VSMCs can modulate their phenotype from contractile (more actin and desmin) to synthesis (less actin and desmin, more vimentin, and higher proliferative activity) (Miyazaki et al, 2002;Owens et al, 2004;Boccardi et al, 2007). Actin, desmin, and vimentin can be detected in both porcine and human aortic samples using the same antibodies, their immunopositivity is robust and therefore easily to be quantified Houdek et al, 2013;Eberlova et al, 2013).…”

Section: Introductionmentioning

confidence: 97%