A proteomic approach to analyzing drought- and salt-responsiveness in rice

Salekdeh, Ghasem Hosseini; Siopongco, Joel D. L. C.; Wade, Leonard; Ghareyazie, Behzad; Bennett, John

doi:10.1016/s0378-4290(02)00040-0

Cited by 245 publications

(170 citation statements)

References 105 publications

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“…Proteome studies allow the examination of the actual products of gene expression and post-translational modifications of proteins in plants tissues (Jiang et al 2007). The influence of salt or osmotic stress on the alterations in proteome was examined in tobacco (Dani et al 2005) and rice leaves (Salekdeh et al 2002). However, there are still only a few studies focused on the triticale proteome.…”

Section: Introductionmentioning

confidence: 99%

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

2013

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Osmotic stress causes many adverse symptoms in plants, which include, for example, growth limitation and decrease or even absence of yield. Proteomic analyses of plant responses to stressors could lead to the introduction of crops with high resistance to osmotic stress. Such plants would be characterized by high yield even under unfavorable environmental conditions. In this article we describe changes in the protein profiles occurring in response to mild and moderate osmotic stress in triticale roots. Analysis of the protein profiles of these roots showed an increased abundance of 14 and a decreased abundance of 11 proteins under mild osmotic stress conditions while a moderate osmotic stress caused an increased abundance of 18 and a decreased abundance of 33 proteins. Twenty-five proteins, whose quantity altered under stress were identified using MALDI-TOF mass spectrometry. The identified proteins were classified into the categories of proteins associated with: defense mechanisms, metabolism, transcription, cell structure, protein synthesis, transport and signal transduction. The functions of identified proteins were discussed in relation to osmotic stress. Some of the identified proteins may be responsible for the adaptation of plants to adverse conditions.

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Section: Introductionmentioning

confidence: 99%

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

2013

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“…Proteomic approach has been applied to study rice responses to salt stress at seedling stage [7,[12][13][14]. The saltresponsive proteins in the roots of the salt-tolerant rice variety Pokkali and the salt-sensitive variety IR29 were studied previously [7].…”

Section: Introductionmentioning

confidence: 99%

“…The saltresponsive proteins in the roots of the salt-tolerant rice variety Pokkali and the salt-sensitive variety IR29 were studied previously [7]. They identified several salt responsive proteins including an ABA-and stress-responsive protein (ASR1), ascorbate peroxidase, and caffeoyl CoA O-methyltransferase.…”

Section: Introductionmentioning

confidence: 99%

Proteomic responses of rice young panicles to salinity

et al. 2006

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Rice (Oryza sativa) is most sensitive to salinity during the reproductive stage. We employed a proteomic approach to further understand the mechanism of plant responses to salinity at an early reproductive stage. Plants were grown in culture solution and salt stress imposed at panicle initiation. After 12 days of stress, young panicles were collected from control and salt stressed plants. The Na + and K + content of panicle and several yield components changed significantly in response to short-term salt stress. The collected panicles were sorted into three different sizes (7 6 1, 11 6 1, and 15 6 1 mm) and their proteome patterns were analyzed using 2-DE in triplicates. The expression pattern of 13 proteins significantly changed in all panicle sizes in response to stress. MS analysis of salt responsive proteins and 16 other highly abundant proteins of panicle led to the identification of proteins involved in several salt responsive mechanisms which may increase plant adaptation to salt stress including higher constitutive expression level and upregulation of antioxidants, up-regulation of proteins involved in translation, transcription, signal transduction, and ATP generation. To the best of our knowledge, this is the first proteome analysis of plant young panicle which may enhance our understanding of plant molecular responses to salinity. Proteome reference map of rice young panicle is available at http://www.proteome.ir.

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“…Preliminary studies on proteomic response to drought and salt stress in rice at IRRI revealed that, apart from an increase in abundance of some stress-responsive proteins, certain other proteins exhibited altered electrophoretic mobility, indicating altered status of PTM (Salekdeh et al, 2002). Manifestation of PTM-mediated responses through protein oligomerization is now being recognized as an important component of biotic and abiotic stress responses.…”

Section: A Strategy For Improving Drought Resistancementioning

confidence: 99%

Drought Resistance Improvement in Rice: An Integrated Genetic and Resource Management Strategy

Serraj

McNally

Slamet‐Loedin

et al. 2011

Plant Production Science

207

116

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Drought is the major constraint to rice production in rainfed areas across Asia and subSaharan Africa. In the context of current and predicted water scarcity, increasing irrigation is generally not a viable option for alleviating drought problems in rainfed rice-growing systems. It is therefore critical that genetic management strategies for drought focus on maximum extraction of available soil moisture and its effi cient use in crop establishment and growth to maximize biomass and yield. Extensive genetic variation for drought resistance exists in rice germplasm. However, the current challenge is to decipher the complexities of drought resistance in rice and exploit all available genetic resources to produce rice varieties combining drought adaptation with high yield potential, quality, and resistance to biotic stresses. The strategy described here aims at developing a pipeline for elite breeding lines and hybrids that can be integrated with efficient management practices and delivered to rice farmers. This involves the development of high-throughput, highprecision phenotyping systems to allow genes for yield components under stress to be effi ciently mapped and their effects assessed on a range of drought-related traits, and then moving the most promising genes into widely grown rice mega-varieties, while scaling up gene detection and delivery for use in marker-aided breeding.

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A proteomic approach to analyzing drought- and salt-responsiveness in rice

Cited by 245 publications

References 105 publications

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

Proteomic responses of rice young panicles to salinity

Drought Resistance Improvement in Rice: An Integrated Genetic and Resource Management Strategy

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