A proteomic approach in the study of an animal model of Parkinson's disease

Iuliis, Angela De; Grigoletto, Jéssica; Recchia, Alessandra; Giusti, Pietro; Arslan, Paola

doi:10.1016/j.cccn.2005.03.028

Cited by 84 publications

(52 citation statements)

References 48 publications

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“…However, significant research using animal models of PD has been completed and will be discussed in this section. By shifting focus to the brain region most severely affected in this disease, the substantia nigra, two classic proteins, a-enolase and b-actin, were identified as being oxidatively modified in a hemiparkinsonian animal model (120). Different animal models of PD are known; however, redox poteomics studies so far were conducted only in A30P (-synuclein transgenic mice.…”

Section: Redox Proteomics In Pdmentioning

confidence: 99%

Redox Proteomics in Selected Neurodegenerative Disorders: From Its Infancy to Future Applications

Butterfield

Perluigi

Reed

et al. 2012

Antioxidants & Redox Signaling

156

142

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Several studies demonstrated that oxidative damage is a characteristic feature of many neurodegenerative diseases. The accumulation of oxidatively modified proteins may disrupt cellular functions by affecting protein expression, protein turnover, cell signaling, and induction of apoptosis and necrosis, suggesting that protein oxidation could have both physiological and pathological significance. For nearly two decades, our laboratory focused particular attention on studying oxidative damage of proteins and how their chemical modifications induced by reactive oxygen species/reactive nitrogen species correlate with pathology, biochemical alterations, and clinical presentations of Alzheimer's disease. This comprehensive article outlines basic knowledge of oxidative modification of proteins and lipids, followed by the principles of redox proteomics analysis, which also involve recent advances of mass spectrometry technology, and its application to selected age-related neurodegenerative diseases. Redox proteomics results obtained in different diseases and animal models thereof may provide new insights into the main mechanisms involved in the pathogenesis and progression of oxidativestress-related neurodegenerative disorders. Redox proteomics can be considered a multifaceted approach that has the potential to provide insights into the molecular mechanisms of a disease, to find disease markers, as well as to identify potential targets for drug therapy. Considering the importance of a better understanding of the cause/effect of protein dysfunction in the pathogenesis and progression of neurodegenerative disorders, this article provides an overview of the intrinsic power of the redox proteomics approach together with the most significant results obtained by our laboratory and others during almost 10 years of research on neurodegenerative disorders since we initiated the field of redox proteomics. Antioxid. Redox Signal. 17, 1610-1655.

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Section: Redox Proteomics In Pdmentioning

confidence: 99%

Redox Proteomics in Selected Neurodegenerative Disorders: From Its Infancy to Future Applications

Butterfield

Perluigi

Reed

et al. 2012

Antioxidants & Redox Signaling

156

142

View full text Add to dashboard Cite

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“…This included a-enolase (ENO1), which was previously shown to undergo increased oxidation in PD and AD-affected brains [122]. The results from the proteomics study by De Iuliis and coworkers suggest that glycolytic changes occur as a compensatory effect in reaction to 6-OHDA-induced oxidative stress seen in rats, which, in rats, replicates the defective METC mechanism proposed to underlie human PD [121].…”

Section: Reviewmentioning

confidence: 69%

“…Using the unihemispheric 6-OHDA rat model of PD, De Iuliis and colleagues combined 2-DE-PAGE with matrixassisted laser desorption/ionizing time-of-flight (MALDI-TOF) MS for separating protein mixtures, visualizing protein patterns and analyzing differentially expressed protein spots [121]. An upregulation in several proteins' relative level of abundance was observed in the SN.…”

Section: Reviewmentioning

confidence: 99%

Mitochondrial proteomics as a selective tool for unraveling Parkinson’s disease pathogenesis

Pienaar¹,

Dexter²,

Burkhard³

2010

Expert Review of Proteomics

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“…Collagen 1, coronin, tau protein, and vimentin were enriched in amyloid plaques [71]. Beta actin showed increased levels in the brain of rats serving as model of Parkinson's disease [117].…”

Section: Structural Proteinsmentioning

confidence: 98%

“…Creatine kinase BB and DRP2 were found to be oxidized in the AD brain [71,72]. In an animal model of Parkinson's disease, increased levels were observed for alpha enolase [117].…”

Section: Energy Metabolism Proteinsmentioning

confidence: 99%

Proteomics‐driven progress in neurodegeneration research

Fountoulakis

Κοσσίδα

2006

Electrophoresis

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Proteomics-driven progress in neurodegeneration researchProteomics technologies have been widely used in the investigation of neurodegenerative and psychiatric disorders, and in particular in the detection of differences between healthy individuals and patients suffering from such diseases. Thus, brain and cerebrospinal fluid (CSF) samples from patients with Alzheimer's disease, Down syndrome, Pick's disease, Parkinson's disease, schizophrenia, and other disorders as well as brain and CSF from animals serving as models of neurological disorders have been analyzed by proteomics. 2-DE followed by MALDI-TOF-MS has been mainly applied as this proteomics approach provides the possibility of convenient quantification of protein levels and detection of post-translational modifications. About 330 unique proteins with deranged levels and modifications have been detected by proteomics approaches to be related to neurodegeneration and psychiatric disorders. They are mainly involved in metabolism pathways, cytoskeleton formation, signal transduction, guidance, detoxification, transport, and conformational changes. In this article, we provide a summary of the major contributions of proteomics technologies in the study of neurodegenerative and psychiatric diseases, in particular, in the detection of changes in protein levels and modifications related to these disorders. IntroductionNeurodegeneration is a major hallmark of a series of genetic and acquired disorders of the central nervous system (CNS), like Alzheimer's disease (AD), Down syndrome (DS), Pick's disease, Creutzfeldt-Jakob disease (CJD), and Parkinson's disease, which result in severe cognitive and motor deficits. A common characteristic of these diseases is that they are multifactorial.Their molecular basis is unknown and individual biomarkers are not reliable. Genomics and proteomics approaches have been applied to obtain a better understanding of neurodegeneration and psychiatric disorders. Genomics has been used to map and identify genes that are mutated in a wide variety of such disorders, like presenilins in AD. Next to genomics, which only provides a partial view of the biological problem, proteomics is widely used in clinical diagnosis as well and many changes in protein levels associated with specific diseases have been identified [1,2].Proteomics is a technology-driven science that studies the proteins of the various biological systems, their structures, interactions, post-translational modifications, and in particular the changes in their levels and modifications, which are the result of specific diseases or are induced by external factors.

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A proteomic approach in the study of an animal model of Parkinson's disease

Cited by 84 publications

References 48 publications

Redox Proteomics in Selected Neurodegenerative Disorders: From Its Infancy to Future Applications

Redox Proteomics in Selected Neurodegenerative Disorders: From Its Infancy to Future Applications

Mitochondrial proteomics as a selective tool for unraveling Parkinson’s disease pathogenesis

Proteomics‐driven progress in neurodegeneration research

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