A Proteomic Analysis of Leaf Responses to Enhanced Ultraviolet-B Radiation in Two Rice (Oryza sativa L.) Cultivars Differing in UV Sensitivity

Abstract

To determine the proteomic response to UV irradiation, two cultivars, i.e., Lemont (UV tolerant) and Dular (UV sensitive), were exposed to natural and enhanced ultraviolet-B (UV-B) irradiation for 1, 7, and 14 days, and two-dimensional gel electrophoresis in combination with mass spectrometry (MS) and bioinformatics were used to compare the different proteomic responses in the leaves of the two cultivars. Thirty-nine proteins were up-or downregulated following the UV-B treatments. Among them, 30 increased or decreased more than 1.5-fold in abundance. They were further tested by using matrix-assisted laser desorption/ionization time of flight MS and performed a database search. Twentyfour proteins were thus identified. These identified proteins were mostly upregulated in Lemont, whereas only 14 of them upregulated in Dular. Nine proteins involved in glycometabolism and fatty acid metabolisms, signal transduction, and protein synthesis and folding in Dular were not changed. These results suggest that there was a complex regulative mechanism on the proteomes in rice leaves upon UV-B exposure.

Effect of UV-B radiation on growth and antioxidative enzymes activity in Lithuanian potato (Solanum tuberosum L.) cultivars

“…4). During recent proteomic studies on Oryza sativa (Wu et al, 2011), 39 proteins upor downregulated, following the UV-B radiation, have been identified. These identified proteins were mostly upregulated in UV-B-tolerant cultivars of rice, while less than half of them were downregulated in UV-B-sensitive cultivars of rice.…”

“…In response to stressors, plant mobilizes various protective materials: antioxidant enzymes, signal and PRproteins, flavonoids and phenylpropanoids, hormones, and this feature can be applied to increase the synthesis of plant-produced biologically valuable materials; however, the response and stress-induced effects depend on the plant species, intraspecific diversity, and genotype characteristics (Kalbina, Strid, 2006;Wu et al, 2011;Baroniya et al, 2013). In our previous studies we demonstrated that interspecific somatic potato hybrids resistant to cold after UV-B 8 kJ m -2 d -1 dose exposure, induced two new SOD isoforms, indicating that hybrids are also more tolerant to another environmental factor -UV-B radiation (Vyšniauskienė et al, 2007).…”

“…Higher doses of UV-B radiation in plant cells increase reactive oxygen species which cause ambivalent plant reactions: a part of reactive oxygen species causes oxidative stress and leads to irreversible oxidative damage of leaf tissues, another part activates the plant protection systems of different character (Mittler, 2002;Kakani et al, 2003). UV effect on plants occurs within the regulatory systems controlling plant response to stresscausing factor (Holley et al, 2003;Wu et al, 2011). Constantly exposed to changing climatic conditions and abiotic factors, plants have developed various protective systems, which directly stimulate the synthesis of protective materials: enzymatic antioxidants (Yannarelli et al, 2006;Baroniya et al, 2013) and non-enzymaticphenolic compounds, flavonoids (Xu, Sullivan, 2010), anthocyanins (Park et al, 2007).…”

“…Differences in UV-B sensitivity between cultivars of the same species have been investigated in rice (Oryza sativa L.) cultivars (Kumagai et al, 2001;Wu et al, 2011), wheat (Triticum aestivum L.) cultivars (Pinto et al, 2000) and cucumber (Cucumis sativus L.) cultivars (Tapia et al, 2010). Knowledge about potato cultivars' susceptibility to UV-B radiation is not yet available.…”

RESPONSES IN NITROGEN MASS AND NITROGEN METABOLISM OF WILD SUGARCANE (<I>SACCHARUM SPONTANEUM</I> L.) CLONES TO ENHANCED UV-B RADIATION UNDER FIELD CONDITIONS

“…UV-B radiation may affect N cycling in ecosystems by decreasing mobilization of N into biomass, photosynthesis, the decomposition rate of litter and the N concentration of plants (Moody et al, 2001;Wu et al, 2011). Moreover, reports about intraspecific responses in N metabolism of plants to enhanced ultraviolet-B radiation under field conditions are limited.…”