A proteomic analysis of IVF follicular fluid in women ≤32 years old

Estes, Stephanie J.; Ye, Bin; Qiu, Weiliang; Cramer, Daniel W.; Hornstein, Mark D.; Missmer, Stacey A.

doi:10.1016/j.fertnstert.2008.08.120

Cited by 75 publications

(60 citation statements)

References 47 publications

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“…In this study, the increased levels of C3 and ZAG in the COH FF suggest that the patients might have had aberrant immune and inflammatory responses during the cycle. The level of C3 in FF has been found to be lower in the women undergoing IVF-ET with a live birth than that in the women with a failed pregnancy [29]. On the other hand, a high follicular level of ZAG was associated with failed pregnancy of the women undergoing IVF-ET [14].…”

Section: Discussionmentioning

confidence: 94%

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Zhang

et al. 2015

J Assist Reprod Genet

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Purpose To study the differences in protein expression profiles of follicular fluid (FF) between controlled ovarian hyperstimulation (COH) and natural ovulatory cycles. Methods Twelve infertile women undergoing in vitro fertilization and embryo transfer (IVF-ET), with matched clinical information, were retrospectively recruited in the IVF center of our university hospital, including six undergoing COH and another six with natural cycles. FF was sampled from dominant follicles with mature oocytes. Protein expression profiles in each FF sample were analyzed respectively using twodimensional gel electrophoresis. Differentially expressed proteins were identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS) and validated by western blotting. Differentially expressed proteins were further analyzed using Ingenuity Pathway Analysis (IPA) software.Results Two proteins were downregulated and 11 proteins were upregulated (change ≥1.5-fold, P<0.05) in the COH group. We identified one down-egulated and seven upregulated proteins using MALDI-TOF MS. Four differentially expressed proteins, including transferrin, complement component C3 (C3), haptoglobin and alpha-1-antitrypsin (AAT), were further validated by rate nephelometry and western blotting analyses. The IPA analysis revealed a significant network involved in the humoral immune and inflammatory responses. Conclusions The eight differentially expressed proteins were related to immune and inflammatory responses in the ovary. Our results provide new insights into the influence of COH on follicular (spp) development and IVF outcomes.

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Section: Discussionmentioning

confidence: 94%

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Zhang

et al. 2015

J Assist Reprod Genet

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“…In a similar study, Aleyasin et al [17] suggested that vitamin D deficiency had no correlation with IVF outcomes. Estes et al [18] demonstrated that proteomic analysis of follicular fluid had potential as a biomarker of good responders versus poor responders in IVF. The current study found that vitamin D in the follicular fluid was the same in the pregnant and non-pregnant groups.…”

Section: Discussionmentioning

confidence: 99%

Value of follicular fluid vitamin D in predicting the pregnancy rate in an IVF program

Firouzabadi

Rahmani

Rahsepar

et al. 2013

Arch Gynecol Obstet

102

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Purpose This study investigated whether there is a correlation between levels of 25OH-D in the follicular fluid and the serum of infertile women and the results of IVF and rates of pregnancy. The association between the levels of 25OH-D in the follicular fluid and the vitamin D repletion status was also assessed. Methods Two hundred and twenty-one infertile women participated in an IVF cycle from 2010 to 2011 in a prospective observational study. Serum and follicular fluid were collected for vitamin D analysis. Deficient, insufficient, and sufficient levels of vitamin D were defined as 10, 10-29, and 30-100 ng/ml, respectively. IVF cycle parameters and clinical pregnancy rates were also compared with the vitamin D level. Results The levels of vitamin D deficiency, insufficiency, and sufficiency were 22.6, 70.1, and 7.2 %, respectively. The fertilization rates associated with these three levels of vitamin D were 43.17, 53.37, and 58.77 %, respectively, (P = 0.054), and the implantation rates were 17.33, 15.26, and 18.75 %, respectively, (P = 0.579). No significant correlation was seen between the pregnancy rate and the serum vitamin D level (P = 0.094) or the follicular vitamin D level (P = 0.170). The serum and follicular fluid vitamin D levels showed a significant correlation (P = 0.000). Conclusion Although vitamin D is an important hormone in the human body, no correlation was found between the serum and follicular vitamin D level and the pregnancy rate in the IVF cycle.

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“…In addition, Missmer et.al, performed a proteomic evaluation of follicular fluids through two-dimensional polyacrylamide gel electrophoresis followed by the Liquid chromatography-tandem mass spectrometry (LC-MS/MS) technique to compare between normoresponders versus poor-responders in matched pairs of IVF patients; however, samples were collected among women 32 years of age or less. In their analysis, they described 11 potential protein candidates for prediction of oocyte normal response [18]. Furthermore, Hanrieder et al, identified 73 unique proteins (significance level of 95%), including acute phase proteins [19], while using an alternative approach of bottom-up technique.…”

Section: Discussionmentioning

confidence: 99%

Decoding the Proteome of In-Vitro Fertilization Ovarian Follicular Fluid for Women Over 35 Years

et al. 2016

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Anahtar Kelimelerİn-Vitro Fertilizasyon; Follikül Sıvısı; Proteom Abstract Aim: The study of follicular fluid using proteomic techniques could provide a useful tool for understanding follicular fluid components and their effect on pregnancy outcome. The aim of the study is to identify and catalog follicular fluid proteins in women 35 years of age or older. Material and Method: Follicular fluid was collected from 21 couples, of which 11 couples achieved successful pregnancy and 10 couples failed to get pregnant. Samples were analyzed by multidimensional chromatography coupled with in-line nano-spray ionization mass spectrometry on an LTQ XL ion trap mass spectrometer. We used the Biomarker Analysis Program from PDQuest software to identify protein constituents in pregnant and non-pregnant groups. Results: In total, 1024 protein specimens were identified. The proteins identified were consistent throughout the experiment and within each of the analyzed specimens. Discussion: A compiled listing of follicular fluid proteins could be a potential starting point for the identification and evaluation of important proteins involved in the development of oocytes; the results of our study may fill a noticeable knowledge-gap in the understanding of follicular fluid proteome.

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A proteomic analysis of IVF follicular fluid in women ≤32 years old

Cited by 75 publications

References 47 publications

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Preliminary proteomic analysis on the alterations in follicular fluid proteins from women undergoing natural cycles or controlled ovarian hyperstimulation

Value of follicular fluid vitamin D in predicting the pregnancy rate in an IVF program

Decoding the Proteome of In-Vitro Fertilization Ovarian Follicular Fluid for Women Over 35 Years

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