A Proteome-wide Screen for Mammalian SxIP Motif-Containing Microtubule Plus-End Tracking Proteins

Jiang, Kai; Toedt, Grischa; Gouveia, Susana Montenegro; Davey, Norman E.; Hua, Shasha; Vaart, Babet van der; Grigoriev, Ilya; Larsen, Jesper; Pedersen, Lotte B.; Bezstarosti, Karel; Lince-Faria, Mariana; Demmers, Jeroen; Steinmetz, Michel O.; Gibson, Toby J.; Akhmanova, Anna

doi:10.1016/j.cub.2012.07.047

Cited by 200 publications

(263 citation statements)

References 37 publications

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“…This is different from the localization seen in interphase non-ciliated cells, where CEP104 localized to the distal ends of both the mother and daughter centrioles (Fig. 6A) (see also Jiang et al, 2012). In non-ciliated cells, the intensity of the CEP104 signal appeared to be similar at both the centrioles.…”

Section: Cep104 the Mammalian Homolog Of Fap256 Moves To The Tip Ducontrasting

confidence: 69%

Centrosomal protein CEP104/ChlamydomonasFAP256 moves to the ciliary tip during cilia assembly

Tammana

Diener

et al. 2013

Journal of Cell Science

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SummaryThe ciliary tip has been implicated in ciliary assembly and disassembly, and signaling, yet information on its protein composition is limited. Using comparative, quantitative proteomics based on the fact that tip proteins will be approximately twice as concentrated in half-length compared with full-length flagella, we have identified FAP256 as a tip protein in Chlamydomonas. FAP256 localizes to the tips of both central pair and outer doublet microtubules (MTs) and it remains at the tip during flagellar assembly and disassembly. Similarly, its vertebrate counterpart, CEP104, localizes on the distal ends of both centrioles of nondividing cells until the mother centriole forms a cilium and then localizes at the tip of the elongating cilium. A null mutant of FAP256 in Chlamydomonas and RNAi in vertebrate cells showed that FAP256/CEP104 is required for ciliogenesis in a high percentage of cells. In those cells that could form cilia, there were structural deformities at the ciliary tips.

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Section: Cep104 the Mammalian Homolog Of Fap256 Moves To The Tip Ducontrasting

confidence: 69%

Centrosomal protein CEP104/ChlamydomonasFAP256 moves to the ciliary tip during cilia assembly

Tammana

Diener

et al. 2013

Journal of Cell Science

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“…Mutations in APC result in mispositioning of the mother centrosome and subsequent defects in spindle orientation (Yamashita et al, 2003 A recent proteomic study identified microtubule affinityregulating kinase 1 (MARK1; also known as PAR-1), a serine/threonine kinase, as another SxIP-containing partner of EB1 ( Fig. 2A,C) (Jiang et al, 2012). In C. elegans, PAR-1 regulates the cortical polarization of factors involved in positioning of the mitotic spindle.…”

mentioning

confidence: 99%

Molecular pathways regulating mitotic spindle orientation in animal cells

2013

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Orientation of the cell division axis is essential for the correct development and maintenance of tissue morphology, both for symmetric cell divisions and for the asymmetric distribution of fate determinants during, for example, stem cell divisions. Oriented cell division depends on the positioning of the mitotic spindle relative to an axis of polarity. Recent studies have illuminated an expanding list of spindle orientation regulators, and a molecular model for how cells couple cortical polarity with spindle positioning has begun to emerge. Here, we review both the wellestablished spindle orientation pathways and recently identified regulators, focusing on how communication between the cell cortex and the spindle is achieved, to provide a contemporary view of how positioning of the mitotic spindle occurs.Key words: Spindle, Microtubules, Oriented cell division, Polarity, Mitosis, Centrosome IntroductionAll multicellular animals are tasked with two fundamental developmental challenges: generating cellular diversity and forming three-dimensional tissues, both of which initiate from a singlecelled zygote. Cellular diversity is spawned by cell divisions yielding non-identical daughters, and tissue morphogenesis is established through the precise three-dimensional arrangement of cell divisions that form the overall architecture of the organism. Both of these essential challenges are resolved in part through oriented cell division, which regulates embryogenesis, organogenesis and cellular differentiation. Notably, oriented cell divisions, and hence asymmetric cell divisions, remain crucial throughout adulthood as well, functioning as the basis for tissue homeostasis during growth and wound repair. One primary feature of oriented cell division is the proper positioning of the mitotic spindle relative to a defined polarity axis. In principle, spindle orientation is achieved through signaling pathways that provide a molecular link between the cell cortex and spindle microtubules. These pathways are thought to elicit both static connections and dynamic forces on the spindle to achieve the desired orientation prior to cell division. Although our knowledge of the signaling molecules involved in this process and our understanding of how they each function at the molecular level remain limited, collective efforts over the years have shed light on the importance of spindle orientation to animal development and function. Moreover, emerging evidence shows an association between improper spindle orientation and a number of developmental diseases as well as tumor formation. The study of spindle orientation is therefore fundamental to both developmental biology and human disease.Over a century ago, Oscar Hertwig discovered that sea urchin embryos biased the orientation of the mitotic spindle along their long axis, which led to a model (the 'Hertwig Rule') in which cells orient divisions in response to mechanical forces (Hertwig, 1884). The Hertwig model stated that mechanical regulation was the primary determinant of spindle orien...

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“…The GAR domain is only found in a few proteins in addition to spectraplakins, such as growth arrest specific protein 2 (GAS2) and the GAS2-like proteins (GAS2L1, GAS2L2 and GAS2L3) (Akhmanova and Steinmetz, 2008;Jiang et al, 2012;Lander et al, 2001;Röper et al, 2002;Stroud et al, 2011Stroud et al, , 2014. It was documented that the GAR domain in spectraplakins associates with and stabilizes MTs, thus helping to link MTs to other parts of the cytoskeleton (Lee and Kolodziej, 2002;Leung et al, 1999;Sun et al, 2001).…”

Section: Actin-binding Domainsmentioning

confidence: 99%

Spectraplakin family proteins – cytoskeletal crosslinkers with versatile roles

Zhang

Yue

2017

Journal of Cell Science

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The different cytoskeletal networks in a cell are responsible for many fundamental cellular processes. Current studies have shown that spectraplakins, cytoskeletal crosslinkers that combine features of both the spectrin and plakin families of crosslinkers, have a critical role in integrating these different cytoskeletal networks. Spectraplakin genes give rise to a variety of isoforms that have distinct functions. Importantly, all spectraplakin isoforms are uniquely able to associate with all three elements of the cytoskeleton, namely, F-actin, microtubules and intermediate filaments. In this Review, we will highlight recent studies that have unraveled their function in a wide range of different processes, from regulating cell adhesion in skin keratinocytes to neuronal cell migration. Taken together, this work has revealed a diverse and indispensable role for orchestrating the function of different cytoskeletal elements in vivo.

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A Proteome-wide Screen for Mammalian SxIP Motif-Containing Microtubule Plus-End Tracking Proteins

Cited by 200 publications

References 37 publications

Centrosomal protein CEP104/ChlamydomonasFAP256 moves to the ciliary tip during cilia assembly

Centrosomal protein CEP104/ChlamydomonasFAP256 moves to the ciliary tip during cilia assembly

Molecular pathways regulating mitotic spindle orientation in animal cells

Spectraplakin family proteins – cytoskeletal crosslinkers with versatile roles

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